ISAeme20
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP007567 | ND | Aeromonas media | Aeromonas media WS |
DNA section
IS Length : 1264 bp
Ends
IR Length : 23/31
IRL : TGAGGTATCCTGATTTTCACAGACAGTCCCGATTGTTACGATGAGACAAT
IRR : TGAGGTGTCCTGACAAGATCGGACAGTCCCGGATCAAGCAGCCTGAGCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGTGAAGGCG | AGTATCGCTT | 0 | |
| ACAACCACCC | AACAGACGCA | 0 |
DNA sequence
TGAGGTATCCTGATTTTCACAGACAGTCCCGATTGTTACGATGAGACAATCGGGGGTATTGAATGAAATCGAGAGAACACTATTCACCAGAGTTTCGGGC
AGAGGCGGTTCGACTGGTGTTGGAGCAAGGCCTTGGCCAAGCTGAAGCCGCCAGAAGGTTGGGTATTGCCAAAGGCACACTGAGCAACTGGGTGGTCGCA
GCCAAAGGAGGAAAGGATACATCGGCCATTCCCGGAGCGCGTTCTGTCGCCGAGCTGGAGGCTGAAGTAGCCCGGCTACGCAAAGCTCTCGCACAAGCCA
ACATGGAGCGCGACATCATAAAAAAGGCCGCCGCGTACTTTGCTCAGGAGTCACTGCCAAGTACGCGTGGATAGCCAACCAACAGGACATCTATCCCATT
TCCGTTATCTGTCGTGTGCTGGAGGTCTCTCGTGCGGGCTTCTACCACTGGCGCCAGCGTCCACCGTCACAGCGCCAACAAGAAGATGAACGACTCAAGG
TCGCCATTCGGGCTGCTCACAGCAAGACGCGCCAGACCTATGGCGCATCGCGTTTACAAGCTGAGTTGGCCGCCGAAGGATTTTACGCTGGGCGGGACCG
GATTGGGCGATTGCGCCGTGAAATGGGGTTGTCTTGCAAACAAAAGCGCAAATTCAAGGCGACAACCCATTCTGCTCATTCCCTGCCCGTGGCGGAGAAT
CTCCTTGGTCAGGTCTTTGCGCCGACGAAGCCCAACGAAGTCTGGACCGGTGATATCACCTATATCGCCACCGACGAAGGCTGGCTTTATCTGGCTGGTC
TCAAGGATGTGTTTACCTGTGAGATAGTGGGTTATGCGATGGGAGAGCGAATGACCACCGGGTTGGTCAGTCAGGCGCTGTTTCGGGCTGTGCAGCAGAA
GAGGCCACCCGTTGGGCTTATTCATCACACAGACCGGGGCAGTCAGTACTGTGCCAAGGCTTACCGGGCTCTCCAGGTACAATTTGGCATGCAGACCTCC
ATGTCACGCAAGGGGAACTGCTTCGACAATGCCCCGATAGAAAGCTTCTGGGGGTCTCTTAAAAATGAACTGGTTCACCACCATAGATTCGAGACGAGAG
CGGAGGCCAAGGCAGCGATTCAGGAATACATAGAGATTTTTTACAACCGCCAGCGTCGGCATTCACGACTTGGCAATGTCAGCCCGGCGGAGTTTAACAA
GAGGTATTGGAGAACTGCTCAGGCTGCTTGATCCGGGACTGTCCGATCTTGTCAGGACACCTCA
AGAGGCGGTTCGACTGGTGTTGGAGCAAGGCCTTGGCCAAGCTGAAGCCGCCAGAAGGTTGGGTATTGCCAAAGGCACACTGAGCAACTGGGTGGTCGCA
GCCAAAGGAGGAAAGGATACATCGGCCATTCCCGGAGCGCGTTCTGTCGCCGAGCTGGAGGCTGAAGTAGCCCGGCTACGCAAAGCTCTCGCACAAGCCA
ACATGGAGCGCGACATCATAAAAAAGGCCGCCGCGTACTTTGCTCAGGAGTCACTGCCAAGTACGCGTGGATAGCCAACCAACAGGACATCTATCCCATT
TCCGTTATCTGTCGTGTGCTGGAGGTCTCTCGTGCGGGCTTCTACCACTGGCGCCAGCGTCCACCGTCACAGCGCCAACAAGAAGATGAACGACTCAAGG
TCGCCATTCGGGCTGCTCACAGCAAGACGCGCCAGACCTATGGCGCATCGCGTTTACAAGCTGAGTTGGCCGCCGAAGGATTTTACGCTGGGCGGGACCG
GATTGGGCGATTGCGCCGTGAAATGGGGTTGTCTTGCAAACAAAAGCGCAAATTCAAGGCGACAACCCATTCTGCTCATTCCCTGCCCGTGGCGGAGAAT
CTCCTTGGTCAGGTCTTTGCGCCGACGAAGCCCAACGAAGTCTGGACCGGTGATATCACCTATATCGCCACCGACGAAGGCTGGCTTTATCTGGCTGGTC
TCAAGGATGTGTTTACCTGTGAGATAGTGGGTTATGCGATGGGAGAGCGAATGACCACCGGGTTGGTCAGTCAGGCGCTGTTTCGGGCTGTGCAGCAGAA
GAGGCCACCCGTTGGGCTTATTCATCACACAGACCGGGGCAGTCAGTACTGTGCCAAGGCTTACCGGGCTCTCCAGGTACAATTTGGCATGCAGACCTCC
ATGTCACGCAAGGGGAACTGCTTCGACAATGCCCCGATAGAAAGCTTCTGGGGGTCTCTTAAAAATGAACTGGTTCACCACCATAGATTCGAGACGAGAG
CGGAGGCCAAGGCAGCGATTCAGGAATACATAGAGATTTTTTACAACCGCCAGCGTCGGCATTCACGACTTGGCAATGTCAGCCCGGCGGAGTTTAACAA
GAGGTATTGGAGAACTGCTCAGGCTGCTTGATCCGGGACTGTCCGATCTTGTCAGGACACCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 312 bp | 103 aa | 63 | 374 | + | No |
Description : First part of the transposase
ORF sequence :
MKSREHYSPEFRAEAVRLVLEQGLGQAEAARRLGIAKGTLSNWVVAAKGGKDTSAIPGARSVAELEAEVARLRKALAQANMERDIIKKAAAYFAQESLPS
TRG
TRG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 321 aa | 266 | 1231 | + | No |
Description : Second part of the transposase
ORF sequence :
SSPATQSSRTSQHGARHHKKGRRVLCSGVTAKYAWIANQQDIYPISVICRVLEVSRAGFYHWRQRPPSQRQQEDERLKVAIRAAHSKTRQTYGASRLQAE
LAAEGFYAGRDRIGRLRREMGLSCKQKRKFKATTHSAHSLPVAENLLGQVFAPTKPNEVWTGDITYIATDEGWLYLAGLKDVFTCEIVGYAMGERMTTGL
VSQALFRAVQQKRPPVGLIHHTDRGSQYCAKAYRALQVQFGMQTSMSRKGNCFDNAPIESFWGSLKNELVHHHRFETRAEAKAAIQEYIEIFYNRQRRHS
RLGNVSPAEFNKRYWRTAQAA
LAAEGFYAGRDRIGRLRREMGLSCKQKRKFKATTHSAHSLPVAENLLGQVFAPTKPNEVWTGDITYIATDEGWLYLAGLKDVFTCEIVGYAMGERMTTGL
VSQALFRAVQQKRPPVGLIHHTDRGSQYCAKAYRALQVQFGMQTSMSRKGNCFDNAPIESFWGSLKNELVHHHRFETRAEAKAAIQEYIEIFYNRQRRHS
RLGNVSPAEFNKRYWRTAQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1169 bp | 389 aa | 63 | 1231 | + | Yes |
Chemistry : DDE
ORF sequence :
MKSREHYSPEFRAEAVRLVLEQGLGQAEAARRLGIAKGTLSNWVVAAKGGKDTSAIPGARSVAELEAEVARLRKALAQANMERDIIKKGRRVLCSGVTAK
YAWIANQQDIYPISVICRVLEVSRAGFYHWRQRPPSQRQQEDERLKVAIRAAHSKTRQTYGASRLQAELAAEGFYAGRDRIGRLRREMGLSCKQKRKFKA
TTHSAHSLPVAENLLGQVFAPTKPNEVWTGDITYIATDEGWLYLAGLKDVFTCEIVGYAMGERMTTGLVSQALFRAVQQKRPPVGLIHHTDRGSQYCAKA
YRALQVQFGMQTSMSRKGNCFDNAPIESFWGSLKNELVHHHRFETRAEAKAAIQEYIEIFYNRQRRHSRLGNVSPAEFNKRYWRTAQAA
YAWIANQQDIYPISVICRVLEVSRAGFYHWRQRPPSQRQQEDERLKVAIRAAHSKTRQTYGASRLQAELAAEGFYAGRDRIGRLRREMGLSCKQKRKFKA
TTHSAHSLPVAENLLGQVFAPTKPNEVWTGDITYIATDEGWLYLAGLKDVFTCEIVGYAMGERMTTGLVSQALFRAVQQKRPPVGLIHHTDRGSQYCAKA
YRALQVQFGMQTSMSRKGNCFDNAPIESFWGSLKNELVHHHRFETRAEAKAAIQEYIEIFYNRQRRHSRLGNVSPAEFNKRYWRTAQAA
Blast result :
Comments
ISAeme20 is 78% aa similar to ISGlo1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694