ISAeme6
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP007567 | ND | Aeromonas media | Aeromonas media WS |
DNA section
IS Length : 1198 bp
Ends
IR Length : 12
IRL : GGTAATCCCCCCGAAAAACCGGGGTGCTCATAAGTAGAATTTTCCCGTAC
IRR : GGTAATCCCCCCATTTTTAGGGGCATTCAAAAGTAGAGGTCATGCGGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGAGGCGTTA | CGGT | CACGAGGCGC | 4 |
DNA sequence
GGTAATCCCCCCGAAAAACCGGGGTGCTCATAAGTAGAATTTTCCCGTACCTTGAATGCAGGAGGTTCTACATGAAAACATCACGCTTTAGCGATGCCCA
GATCATCGCCATTCTCAACCAGGCTGAGGCCGGCTCGCCCGTCCCGGAGCTCTGCCGCGAGCATGGCATCAGTACCGCCACCTTCTACAAGTGGCGTTCC
AAATTCGGTGGCATGGATGCCTCCCTCATGGCTCGTCTCAAGGAGTTGGAGGACGAGAATCGCCGCCTCAAGAAGATGTACGCCGAGGAGCGCCTCAAGG
CCGAGATCATTGCCGAGGCCATGGCAAAAAAGTGGTGACGCCATCTGCCCGTCGGGAGATGGCGACCAAAGCCGTGGCTCACTTCGCCATATCCATCCGC
CTGGCTTGCCAGGTGTTCAAGGTCAGCGAGAGCTGTTATCGCTACCAACCCGTGCTGGCCGATGAAAACCGGGAGATTGCCGACTGGTTGCTACGTATCA
CCGCCAGCCAGCGCAACTGGGGCTTCGGTCTCTGCTTTCTTTACCTGCGCAACGTCAAAGGGTTTGGCTGGAACCATAAGCGGGTGTACCGGATCTATCG
GGAATTGGAACTCAATCTGCGGATAAAACCCAGGAAGCGATTGGTACGTGCCAAACCGGAGGCCTTGGCGGTTCCCGCCCAGCCCAACCTTTGCTGGTCG
ATGGACTTCATGCACGACCAGCTCAGCGACGGCCGTTCGGTCCGACTGTTTAATGTGATCGACGATTTTAATCGCGAGGCGCTGTGTATCGAGGTGGACT
TCTCCTTGCCGGCGTCACGGGTCAAGCGGGCGCTGGAGCAAGTGATCGCATGGCGCGGCAAGCCGGCGATGATCCGCTGCGATAACGGCCCGGAGTACAT
CAGTGAAGAGCTGAAAAGATGGGCTGAAAAACAGGAGATCCGGCTTGGGTATATCCAGCCGGGAAACCCGCAGCAGAACGCATATATCGAACGGTTTAAC
CGAACGGTACGCTACGACTGGCTGGGTCATTACCTGTTTGAGTCACTGGAGGAGTTACAGGTATTTGCAACAGAATGGCAGTGGATTTATAACCATGAGC
GGCCCAATATGGCGCTGGGTGGGTACACGCCAAAACAGTACCTGGCACAGGCCGCATGACCTCTACTTTTGAATGCCCCTAAAAATGGGGGGATTACC
GATCATCGCCATTCTCAACCAGGCTGAGGCCGGCTCGCCCGTCCCGGAGCTCTGCCGCGAGCATGGCATCAGTACCGCCACCTTCTACAAGTGGCGTTCC
AAATTCGGTGGCATGGATGCCTCCCTCATGGCTCGTCTCAAGGAGTTGGAGGACGAGAATCGCCGCCTCAAGAAGATGTACGCCGAGGAGCGCCTCAAGG
CCGAGATCATTGCCGAGGCCATGGCAAAAAAGTGGTGACGCCATCTGCCCGTCGGGAGATGGCGACCAAAGCCGTGGCTCACTTCGCCATATCCATCCGC
CTGGCTTGCCAGGTGTTCAAGGTCAGCGAGAGCTGTTATCGCTACCAACCCGTGCTGGCCGATGAAAACCGGGAGATTGCCGACTGGTTGCTACGTATCA
CCGCCAGCCAGCGCAACTGGGGCTTCGGTCTCTGCTTTCTTTACCTGCGCAACGTCAAAGGGTTTGGCTGGAACCATAAGCGGGTGTACCGGATCTATCG
GGAATTGGAACTCAATCTGCGGATAAAACCCAGGAAGCGATTGGTACGTGCCAAACCGGAGGCCTTGGCGGTTCCCGCCCAGCCCAACCTTTGCTGGTCG
ATGGACTTCATGCACGACCAGCTCAGCGACGGCCGTTCGGTCCGACTGTTTAATGTGATCGACGATTTTAATCGCGAGGCGCTGTGTATCGAGGTGGACT
TCTCCTTGCCGGCGTCACGGGTCAAGCGGGCGCTGGAGCAAGTGATCGCATGGCGCGGCAAGCCGGCGATGATCCGCTGCGATAACGGCCCGGAGTACAT
CAGTGAAGAGCTGAAAAGATGGGCTGAAAAACAGGAGATCCGGCTTGGGTATATCCAGCCGGGAAACCCGCAGCAGAACGCATATATCGAACGGTTTAAC
CGAACGGTACGCTACGACTGGCTGGGTCATTACCTGTTTGAGTCACTGGAGGAGTTACAGGTATTTGCAACAGAATGGCAGTGGATTTATAACCATGAGC
GGCCCAATATGGCGCTGGGTGGGTACACGCCAAAACAGTACCTGGCACAGGCCGCATGACCTCTACTTTTGAATGCCCCTAAAAATGGGGGGATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSRFSDAQIIAILNQAEAGSPVPELCREHGISTATFYKWRSKFGGMDASLMARLKELEDENRRLKKMYAEERLKAEIIAEAMAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1032 bp | 343 aa | 128 | 1159 | + | No |
Description : Second part of the transposase
ORF sequence :
GRLARPGALPRAWHQYRHLLQVAFQIRWHGCLPHGSSQGVGGRESPPQEDVRRGAPQGRDHCRGHGKKVVTPSARREMATKAVAHFAISIRLACQVFKVS
ESCYRYQPVLADENREIADWLLRITASQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRELELNLRIKPRKRLVRAKPEALAVPAQPNLCWSMDFMHDQLS
DGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVIAWRGKPAMIRCDNGPEYISEELKRWAEKQEIRLGYIQPGNPQQNAYIERFNRTVRYDWLG
HYLFESLEELQVFATEWQWIYNHERPNMALGGYTPKQYLAQAA
ESCYRYQPVLADENREIADWLLRITASQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRELELNLRIKPRKRLVRAKPEALAVPAQPNLCWSMDFMHDQLS
DGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVIAWRGKPAMIRCDNGPEYISEELKRWAEKQEIRLGYIQPGNPQQNAYIERFNRTVRYDWLG
HYLFESLEELQVFATEWQWIYNHERPNMALGGYTPKQYLAQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1088 bp | 362 aa | 72 | 1159 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSRFSDAQIIAILNQAEAGSPVPELCREHGISTATFYKWRSKFGGMDASLMARLKELEDENRRLKKMYAEERLKAEIIAEAMAKKVVTPSARREMATK
AVAHFAISIRLACQVFKVSESCYRYQPVLADENREIADWLLRITASQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRELELNLRIKPRKRLVRAKPEALA
VPAQPNLCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVIAWRGKPAMIRCDNGPEYISEELKRWAEKQEIRLGYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLEELQVFATEWQWIYNHERPNMALGGYTPKQYLAQAA
AVAHFAISIRLACQVFKVSESCYRYQPVLADENREIADWLLRITASQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRELELNLRIKPRKRLVRAKPEALA
VPAQPNLCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVIAWRGKPAMIRCDNGPEYISEELKRWAEKQEIRLGYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLEELQVFATEWQWIYNHERPNMALGGYTPKQYLAQAA
Blast result :
Comments
ISAeme6 is 95% aa similar to ISKpn10.
In the genome, this element is targetted by ISAeme5.
In the genome, this element is targetted by ISAeme5.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694