ISAeme23
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP007567 | ND | Aeromonas media | Aeromonas media WS |
DNA section
IS Length : 1557 bp
Ends
IR Length : 23/27
IRL : GTAACCGTTCACCAGCCCCCCGTTGACAGTCAAATATGACGATCATCTCT
IRR : GTAACCGTTCACCGGCCCCGGCTTGACTAAGCCGAGGTCAGGTTGAATAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTATGTGGTG | AATCTGGGGA | 0 | |
| CTCTGTGAAG | GGGGGATTCA | 0 |
DNA sequence
GTAACCGTTCACCAGCCCCCCGTTGACAGTCAAATATGACGATCATCTCTTGATCCACTTTCGGGGGTTGATAGATCCTCCCAGTCCGTCAACATAAGTC
CATGAACAAGAAAAAACTCTTCGAAAATGCAGCGCCTCAGGCGCACGACCTCCATGATGCCAACCGCATCATCCAGGAGCTATGGAACAAGCTACGCGAG
TACGAGGACCGTCTGGCTCTCTCTTCTCGTAACTCGTCCAAATCACCATCCTCCGACTCGCCCGCGGCTAAGGCGGAGCGAAAAAAGTTGCGACGCCTCG
TGGCCGAAATCAGGTTGGCGCTCAACCTGGACACCCGGGGCATCGACGAGAACTAAGTCTGCTGCAATCCACCGATCAGGTGGTTGCTTGCAAACCGGCA
TCCTGCTGCGCTGAATGTGGCGGAGAGGTCGTGGCCAATGCCGCGCCATCCTTCCGTCACCAGGTGTTTGAACTGCCCAAGTTGCCGCTGGAAATCACCG
AATACCAGGTATTCCACGGCCACTGTCAGCACTGCGATGCGCTCAGCAAAGGCGAACTGCCCAATTCGGCACCCAACGGCCAAATGGGACCGCGGTTGCT
TAGCCATATCGCCGTGCTAGCAGGGAAATATCATCTGAGTGTGCGCAAAATTCAGAGTTTGCTGCACGATCAGTACGGCATGACCTTTTCGGTGGGCGCC
ATCTCGGAAGCCCAGGGGCGTATCAGCTCGATGCTGACCCCCATCCATCAAGCGCTGAAGCAACATATTCAGAAGGCGCCGATCATCCATGCCGATGAGA
CCACGCATCAGCGCAATGGCGAGTCGAATACCCGGTGGTTGTGGTTGATGGCCAGCAACGATGCGGTGTTCCAGAACATTCGTTACTTCCGCAGTCAGGA
CAATGCCAAGCATCTGCTGGGTTCGGCACCCCACGGCGTTGTGGTTACCGACCAGTGCGCCAGTTACCACTGGCTGGATCCGACACGGCATCAATTCTGC
CTGGCGCATGTCCAGCGCAACCTGCAAGAGATGGCGGATTATGGCGGCGGCGGTCAGACAGCCTACCTAGGACATCGGCTGGTCGTGCTGTTGAAGACGG
TCTTTCGCACCCAGCACCGTTACGAGTCGGGGCTGATCGGTGAACAGATCAGACTCAGACGGATGGAGCGATTACGGCGCAGTATCAAGACGGTACTGTG
CAAGGGTGCCGAGGTTCCCGTGCGACGTTATGCCGGTCGCTGCCAGCATATTCTCAAGTATGAAACCGGGCTGTGGGTATTCTTGCAGCATCCAGGGACA
CCGCTGACCAACAACGAGGCGGAGCGGTGTATTCGCGGCAGTGTGATCCTGCGGAAGATCTGTTTCGGCACGAGTTCAGACCGGGGAGACAAGTTCCGCT
CGCGGGTGCTGTCGGTGATTGAGACGAGCAAGAAGCGCCAGCTGTCGGCACTCGAAGTGCTGTGCCGGATCGTCACAGCGGTCATGACAGGCCAGCCCTA
TCCTGACGTATTCAACCTGACCTCGGCTTAGTCAAGCCGGGGCCGGTGAACGGTTAC
CATGAACAAGAAAAAACTCTTCGAAAATGCAGCGCCTCAGGCGCACGACCTCCATGATGCCAACCGCATCATCCAGGAGCTATGGAACAAGCTACGCGAG
TACGAGGACCGTCTGGCTCTCTCTTCTCGTAACTCGTCCAAATCACCATCCTCCGACTCGCCCGCGGCTAAGGCGGAGCGAAAAAAGTTGCGACGCCTCG
TGGCCGAAATCAGGTTGGCGCTCAACCTGGACACCCGGGGCATCGACGAGAACTAAGTCTGCTGCAATCCACCGATCAGGTGGTTGCTTGCAAACCGGCA
TCCTGCTGCGCTGAATGTGGCGGAGAGGTCGTGGCCAATGCCGCGCCATCCTTCCGTCACCAGGTGTTTGAACTGCCCAAGTTGCCGCTGGAAATCACCG
AATACCAGGTATTCCACGGCCACTGTCAGCACTGCGATGCGCTCAGCAAAGGCGAACTGCCCAATTCGGCACCCAACGGCCAAATGGGACCGCGGTTGCT
TAGCCATATCGCCGTGCTAGCAGGGAAATATCATCTGAGTGTGCGCAAAATTCAGAGTTTGCTGCACGATCAGTACGGCATGACCTTTTCGGTGGGCGCC
ATCTCGGAAGCCCAGGGGCGTATCAGCTCGATGCTGACCCCCATCCATCAAGCGCTGAAGCAACATATTCAGAAGGCGCCGATCATCCATGCCGATGAGA
CCACGCATCAGCGCAATGGCGAGTCGAATACCCGGTGGTTGTGGTTGATGGCCAGCAACGATGCGGTGTTCCAGAACATTCGTTACTTCCGCAGTCAGGA
CAATGCCAAGCATCTGCTGGGTTCGGCACCCCACGGCGTTGTGGTTACCGACCAGTGCGCCAGTTACCACTGGCTGGATCCGACACGGCATCAATTCTGC
CTGGCGCATGTCCAGCGCAACCTGCAAGAGATGGCGGATTATGGCGGCGGCGGTCAGACAGCCTACCTAGGACATCGGCTGGTCGTGCTGTTGAAGACGG
TCTTTCGCACCCAGCACCGTTACGAGTCGGGGCTGATCGGTGAACAGATCAGACTCAGACGGATGGAGCGATTACGGCGCAGTATCAAGACGGTACTGTG
CAAGGGTGCCGAGGTTCCCGTGCGACGTTATGCCGGTCGCTGCCAGCATATTCTCAAGTATGAAACCGGGCTGTGGGTATTCTTGCAGCATCCAGGGACA
CCGCTGACCAACAACGAGGCGGAGCGGTGTATTCGCGGCAGTGTGATCCTGCGGAAGATCTGTTTCGGCACGAGTTCAGACCGGGGAGACAAGTTCCGCT
CGCGGGTGCTGTCGGTGATTGAGACGAGCAAGAAGCGCCAGCTGTCGGCACTCGAAGTGCTGTGCCGGATCGTCACAGCGGTCATGACAGGCCAGCCCTA
TCCTGACGTATTCAACCTGACCTCGGCTTAGTCAAGCCGGGGCCGGTGAACGGTTAC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 255 bp | 84 aa | 102 | 356 | + | No |
Description : First part of the transposase
ORF sequence :
MNKKKLFENAAPQAHDLHDANRIIQELWNKLREYEDRLALSSRNSSKSPSSDSPAAKAERKKLRRLVAEIRLALNLDTRGIDEN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1260 bp | 419 aa | 272 | 1531 | + | No |
Description : Second part of the transposase
ORF sequence :
GGAKKVATPRGRNQVGAQPGHPGHRRELSLLQSTDQVVACKPASCCAECGGEVVANAAPSFRHQVFELPKLPLEITEYQVFHGHCQHCDALSKGELPNSA
PNGQMGPRLLSHIAVLAGKYHLSVRKIQSLLHDQYGMTFSVGAISEAQGRISSMLTPIHQALKQHIQKAPIIHADETTHQRNGESNTRWLWLMASNDAVF
QNIRYFRSQDNAKHLLGSAPHGVVVTDQCASYHWLDPTRHQFCLAHVQRNLQEMADYGGGGQTAYLGHRLVVLLKTVFRTQHRYESGLIGEQIRLRRMER
LRRSIKTVLCKGAEVPVRRYAGRCQHILKYETGLWVFLQHPGTPLTNNEAERCIRGSVILRKICFGTSSDRGDKFRSRVLSVIETSKKRQLSALEVLCRI
VTAVMTGQPYPDVFNLTSA
PNGQMGPRLLSHIAVLAGKYHLSVRKIQSLLHDQYGMTFSVGAISEAQGRISSMLTPIHQALKQHIQKAPIIHADETTHQRNGESNTRWLWLMASNDAVF
QNIRYFRSQDNAKHLLGSAPHGVVVTDQCASYHWLDPTRHQFCLAHVQRNLQEMADYGGGGQTAYLGHRLVVLLKTVFRTQHRYESGLIGEQIRLRRMER
LRRSIKTVLCKGAEVPVRRYAGRCQHILKYETGLWVFLQHPGTPLTNNEAERCIRGSVILRKICFGTSSDRGDKFRSRVLSVIETSKKRQLSALEVLCRI
VTAVMTGQPYPDVFNLTSA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1430 bp | 476 aa | 102 | 1531 | + | Yes |
Chemistry : DDE
ORF sequence :
MNKKKLFENAAPQAHDLHDANRIIQELWNKLREYEDRLALSSRNSSKSPSSDSPAAKAERKKVATPRGRNQVGAQPGHPGHRRELSLLQSTDQVVACKPA
SCCAECGGEVVANAAPSFRHQVFELPKLPLEITEYQVFHGHCQHCDALSKGELPNSAPNGQMGPRLLSHIAVLAGKYHLSVRKIQSLLHDQYGMTFSVGA
ISEAQGRISSMLTPIHQALKQHIQKAPIIHADETTHQRNGESNTRWLWLMASNDAVFQNIRYFRSQDNAKHLLGSAPHGVVVTDQCASYHWLDPTRHQFC
LAHVQRNLQEMADYGGGGQTAYLGHRLVVLLKTVFRTQHRYESGLIGEQIRLRRMERLRRSIKTVLCKGAEVPVRRYAGRCQHILKYETGLWVFLQHPGT
PLTNNEAERCIRGSVILRKICFGTSSDRGDKFRSRVLSVIETSKKRQLSALEVLCRIVTAVMTGQPYPDVFNLTSA
SCCAECGGEVVANAAPSFRHQVFELPKLPLEITEYQVFHGHCQHCDALSKGELPNSAPNGQMGPRLLSHIAVLAGKYHLSVRKIQSLLHDQYGMTFSVGA
ISEAQGRISSMLTPIHQALKQHIQKAPIIHADETTHQRNGESNTRWLWLMASNDAVFQNIRYFRSQDNAKHLLGSAPHGVVVTDQCASYHWLDPTRHQFC
LAHVQRNLQEMADYGGGGQTAYLGHRLVVLLKTVFRTQHRYESGLIGEQIRLRRMERLRRSIKTVLCKGAEVPVRRYAGRCQHILKYETGLWVFLQHPGT
PLTNNEAERCIRGSVILRKICFGTSSDRGDKFRSRVLSVIETSKKRQLSALEVLCRIVTAVMTGQPYPDVFNLTSA
Blast result :
Comments
ISAeme23 is 80% aa similar to ISSbe3.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694