ISPa133
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| FJ387166 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa 116136 |
DNA section
IS Length : 1229 bp
Ends
IR Length : 17/21
IRL : TGGTCTAATTGGAGCGGACACCCCGGTAAGCCTCATACTGGAGGCATTGG
IRR : TGGTCAAGTCGTAGCGGACACTTTTATTAAGCTGCTTCCGCGATTTTCTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACTAGCCGTT | GAAC | ACTATCGTCA | 4 |
DNA sequence
TGGTCTAATTGGAGCGGACACCCCGGTAAGCCTCATACTGGAGGCATTGGAGGTGTTCATGACCAAGAAGACGCGACGTCGGTACTCCGACGAATTCAAG
GCTGAGGCGGTCAACATGGTTCGAGGAGAGGGTTATGCCATCTCCGAGGCTGCCAGGCGGCTGGATATCGATCGCAGCCTGCTAGATCGCTGGTGTCGCC
AGCAACGTGACCGGGAGGATGGTGTGACTGACGACCAGGCAGATGAGCGGGATGCCGAGATCAAGAAACTTCGCGAAGAAGTTCGTAAGTTGCGGATTGA
AAAGGAGGTTTTAAAAAAGGCCACGGCCTTCTTCGCGAGAGAGTCGAGCTGAGATACCAGTTCATCGAGTCGGAGAAGGCCTACTTCCCAGTGGCGGTGC
TGTGCCGTGTCATGGACGTCAGCCGCAGTGGCTTCTATGCCTGGCGTCAGCGTGAACCCGATGAGCAGCGCCAGTGCCTGCACCGGGAGGTCAAGGACAT
CCACGATCAGAAACGTGGTAGCTATGGGAGCCGGCGAATGGCCAAGGAACTCCGTCGCAGAGGTCATGCGGTCGGTCGTTATCAGGCGCGTAGCCTGATG
CAGGAAGCGGGTGTCGCCTGCCGACAGCGTCGCCGCTACCGGCATACGACGGACAGCGACCACGGGCTGCCAGTGGCTCCCAACCTGCTCAAGCGGCAGT
TCACGGTGCCTGAGCCCAATCAGCGGTGGGTGGCCGACATCACTGCCATTTGGACCTTGGAAGGCTGGTTGTACCTGGCGGCCGTGCTGGACCTCTACGA
CCGACAGGTCATCGGCTGGGCCATGGCCGACCATATGAAGACGTCACTCACCCTGGACGCCCTGGAGATGGCAATTGGCCGCCGACGGCCTCCCCGGGGA
GTGCTCCACCACTCGGATCGCGGCAGCCAGTACGCCTCTCGTGCGTATCGCGAACGACTGGCAAAGCACGGGTTCCAGGCCTCCATGAGCCGCAAGGGGA
ACTGTTGGGACAATGCGGTCATGGAGCGCTTCTTCGGCTCTCTCAAGAGCGAATGGCTGGAGGGACAGCGGTATTGGACCCGCCAGTCAGCGAGGCGCGA
CGTCGTGACCTATATCGAGATGGAGTACAACAGCTGCCGGCTCCACTCGACCCTTGGCTACCACCCGCCGAGGGAGATCGAGAAAATCGCGGAAGCAGCT
TAATAAAAGTGTCCGCTACGACTTGACCA
GCTGAGGCGGTCAACATGGTTCGAGGAGAGGGTTATGCCATCTCCGAGGCTGCCAGGCGGCTGGATATCGATCGCAGCCTGCTAGATCGCTGGTGTCGCC
AGCAACGTGACCGGGAGGATGGTGTGACTGACGACCAGGCAGATGAGCGGGATGCCGAGATCAAGAAACTTCGCGAAGAAGTTCGTAAGTTGCGGATTGA
AAAGGAGGTTTTAAAAAAGGCCACGGCCTTCTTCGCGAGAGAGTCGAGCTGAGATACCAGTTCATCGAGTCGGAGAAGGCCTACTTCCCAGTGGCGGTGC
TGTGCCGTGTCATGGACGTCAGCCGCAGTGGCTTCTATGCCTGGCGTCAGCGTGAACCCGATGAGCAGCGCCAGTGCCTGCACCGGGAGGTCAAGGACAT
CCACGATCAGAAACGTGGTAGCTATGGGAGCCGGCGAATGGCCAAGGAACTCCGTCGCAGAGGTCATGCGGTCGGTCGTTATCAGGCGCGTAGCCTGATG
CAGGAAGCGGGTGTCGCCTGCCGACAGCGTCGCCGCTACCGGCATACGACGGACAGCGACCACGGGCTGCCAGTGGCTCCCAACCTGCTCAAGCGGCAGT
TCACGGTGCCTGAGCCCAATCAGCGGTGGGTGGCCGACATCACTGCCATTTGGACCTTGGAAGGCTGGTTGTACCTGGCGGCCGTGCTGGACCTCTACGA
CCGACAGGTCATCGGCTGGGCCATGGCCGACCATATGAAGACGTCACTCACCCTGGACGCCCTGGAGATGGCAATTGGCCGCCGACGGCCTCCCCGGGGA
GTGCTCCACCACTCGGATCGCGGCAGCCAGTACGCCTCTCGTGCGTATCGCGAACGACTGGCAAAGCACGGGTTCCAGGCCTCCATGAGCCGCAAGGGGA
ACTGTTGGGACAATGCGGTCATGGAGCGCTTCTTCGGCTCTCTCAAGAGCGAATGGCTGGAGGGACAGCGGTATTGGACCCGCCAGTCAGCGAGGCGCGA
CGTCGTGACCTATATCGAGATGGAGTACAACAGCTGCCGGCTCCACTCGACCCTTGGCTACCACCCGCCGAGGGAGATCGAGAAAATCGCGGAAGCAGCT
TAATAAAAGTGTCCGCTACGACTTGACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 59 | 352 | + | No |
Description : First part of the transposase
ORF sequence :
MTKKTRRRYSDEFKAEAVNMVRGEGYAISEAARRLDIDRSLLDRWCRQQRDREDGVTDDQADERDAEIKKLREEVRKLRIEKEVLKKATAFFARESS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 903 bp | 300 aa | 301 | 1203 | + | No |
Description : Second part of the transposase
ORF sequence :
KGGFKKGHGLLRERVELRYQFIESEKAYFPVAVLCRVMDVSRSGFYAWRQREPDEQRQCLHREVKDIHDQKRGSYGSRRMAKELRRRGHAVGRYQARSLM
QEAGVACRQRRRYRHTTDSDHGLPVAPNLLKRQFTVPEPNQRWVADITAIWTLEGWLYLAAVLDLYDRQVIGWAMADHMKTSLTLDALEMAIGRRRPPRG
VLHHSDRGSQYASRAYRERLAKHGFQASMSRKGNCWDNAVMERFFGSLKSEWLEGQRYWTRQSARRDVVTYIEMEYNSCRLHSTLGYHPPREIEKIAEAA
QEAGVACRQRRRYRHTTDSDHGLPVAPNLLKRQFTVPEPNQRWVADITAIWTLEGWLYLAAVLDLYDRQVIGWAMADHMKTSLTLDALEMAIGRRRPPRG
VLHHSDRGSQYASRAYRERLAKHGFQASMSRKGNCWDNAVMERFFGSLKSEWLEGQRYWTRQSARRDVVTYIEMEYNSCRLHSTLGYHPPREIEKIAEAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1145 bp | 381 aa | 59 | 1203 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKKTRRRYSDEFKAEAVNMVRGEGYAISEAARRLDIDRSLLDRWCRQQRDREDGVTDDQADERDAEIKKLREEVRKLRIEKEVLKKGHGLLRERVELRY
QFIESEKAYFPVAVLCRVMDVSRSGFYAWRQREPDEQRQCLHREVKDIHDQKRGSYGSRRMAKELRRRGHAVGRYQARSLMQEAGVACRQRRRYRHTTDS
DHGLPVAPNLLKRQFTVPEPNQRWVADITAIWTLEGWLYLAAVLDLYDRQVIGWAMADHMKTSLTLDALEMAIGRRRPPRGVLHHSDRGSQYASRAYRER
LAKHGFQASMSRKGNCWDNAVMERFFGSLKSEWLEGQRYWTRQSARRDVVTYIEMEYNSCRLHSTLGYHPPREIEKIAEAA
QFIESEKAYFPVAVLCRVMDVSRSGFYAWRQREPDEQRQCLHREVKDIHDQKRGSYGSRRMAKELRRRGHAVGRYQARSLMQEAGVACRQRRRYRHTTDS
DHGLPVAPNLLKRQFTVPEPNQRWVADITAIWTLEGWLYLAAVLDLYDRQVIGWAMADHMKTSLTLDALEMAIGRRRPPRGVLHHSDRGSQYASRAYRER
LAKHGFQASMSRKGNCWDNAVMERFFGSLKSEWLEGQRYWTRQSARRDVVTYIEMEYNSCRLHSTLGYHPPREIEKIAEAA
Blast result :
Comments
ISPa133 is 67% aa similar to ISNieu3. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2012)
2] Ruiz-Martinez,L., Lopez-Jimenez,L., d'Ostuni,V., Fuste,E., Vinuesa,T. and Vinas,M. (2011) Int. Microbiol. 14, 51-58 .
2] Ruiz-Martinez,L., Lopez-Jimenez,L., d'Ostuni,V., Fuste,E., Vinuesa,T. and Vinas,M. (2011) Int. Microbiol. 14, 51-58 .