ISAeme22

  • Family IS4
  • Group IS50
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1534 bp

Ends


IR Length : 16/26

IRL : CTGACTCTTATACACAAGTAGCGCCCTGAACGGAACCATTCCCGTTTTCC
IRR : CTGTCTCTTGATCAGATCTTGATCCCCTGCGCCATCAGATCCTTGGCGGC

Insertion site


Left flankDirect repeatRight flankDR Length
GAGTTTAGCTATCCAAACCCAGCCCCCTGC10

DNA sequence

CTGACTCTTATACACAAGTAGCGCCCTGAACGGAACCATTCCCGTTTTCCAGGATCTGATCTTCCATGTGACCTCCTAACATGGTAACGATCATGATAAC
TTCTGCTCTTCATCGTGCGGCTGACTGGGCTAAATCTGTGTTCTCTTCGGCGGCGCTGGGTGATCCTCGCCGTACCACCCGTCTGGTTAACGTCGCCGCT
CAATTGGCAAAATGTTCTGGAAAATCAATAACCATCTCATCAGAGGGCTGTGAAGCCATGCAGGAAGGCGCTTACCGATTTATCCGCAATCCCAACGTCT
CTGCCGAGGCGATCAGAAAGGCTGGCGCCATGCAAACCGTCAAGTTAGCTCAGGCGTTTCCCGAACTGCTGGCCATTGAGGACACCACTTCTTTGAGTTA
TCGCCACCAGGTCGCCGAAGAGCTTGGCAAGCTGGGTGCTATTGGAGATAAATCCCGCGGATGGTGGGTTCACTCCGTTCTCTTGCTCGAGGCCACTACA
TTCCGCACCGTAGGATTACTGCATCAGGAGTGGTGGATGCGCCCGGATGACCCTGCCGACGCGGATGAAAAGGAGAGTGGCAAATGGCTGGCTGCGGCCG
CAACTAGCCGGTTACGCATGGGCAGTATGATGAGCAACGTGATTGCGGTCTGTGACCGCGAAGCCGATATCCATGCTTACCTGCAGGACAAACTGACACA
TAAAGAGCGATTTGTAGTGCGCTCCAAGCACCCGCGCAAGGACACTGGCTCCGGATTGTATCTGTACGACCATCTGAAAAACCAACCGGAGCTGGGTGGC
TATCAGATCAGCATCCCGCAAAAGGGCGTGGTGGATAAACGCGGTAAACGTAAAAATCGACCCGCCCGCAAGGCGAGCTTGAGCCTGCGCAGTGGGCGCA
TCACGCTAAAACAGGGGAATATCACGCTCAATGCGGTGTTGGCCGAGGAGATTAACCCGCCCAAGGGTGAGACCCCGTTGAAATGGTTGTTGTTAACCAG
TGAACCCGTCGAGTCACTAGCCCAAGCCTTGCGGGTCATCGACATATACACCCATCGCTGGCGGATCGAGGAGTTCCATAAGGCATGGAAAACCGGAGCT
GGAGCCGAGAGGCAACGCATGGAGGAGCCGGATAATCTGGAGCGGATGGTCTCGATCCTCTCGTTTGTCGCGGTCAGGCTGTTACAGCTCAGAGAAAGCT
TCACGCTGCCGCAAGCACTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCAGCTGAAACGGTGCTGACTCAGGATGAATGTCA
GCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCCAGACTGGGCGGTTTTATG
GACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGGAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGA
TGGCGCAGGGGATCAAGATCTGATCAAGAGACAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1431 bp476 aa931523+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MITSALHRAADWAKSVFSSAALGDPRRTTRLVNVAAQLAKCSGKSITISSEGCEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQAFPELLAIEDTTS
LSYRHQVAEELGKLGAIGDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDK
LTHKERFVVRSKHPRKDTGSGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNAVLAEEINPPKGETPLKWLL
LTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTQD
ECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQSKLDGFLAAKDLMAQGIKI

 

Blast result :
Comments
ISAeme22 is 97% aa similar to IS50R.
IS_pep_1 is B224_6013.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694