ISAeme18

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1075 bp

Ends


IR Length : 21/28

IRL : CGCGAATTCAGATAATAAGTGCAACGCTCGGTCATGGTGCGGAGGAGGGA
IRR : GGCGGATTCAACTCCGAAGTGCAACGCTAAGCCGCCTTTCTTAGCTCCGC

Insertion site


Left flankDirect repeatRight flankDR Length
AAAAGCTGGGATTCTTTGGT0

DNA sequence

CGCGAATTCAGATAATAAGTGCAACGCTCGGTCATGGTGCGGAGGAGGGAGTTAGCTGCTGATAAGCTATCCCTCTTCTGTCCAAAGATGAGCGAGTCAC
CATGAAATATCAGCAGCTAACTGCGGGGCTACGGTACCAGATTGCCCTGCTCTACGGCGAGAGTTTTAGTTGTAGAGAGATAGGGCTACGGGTCGGGGTC
AGTAAGTCGACGGTTTCCCGTGAACTCAGGCGAAACAGCACTCCCAATGGTTATGCACCTGATGAGGCGCAGCGCCTGAGTGAGCTGCGACGCTCTGGCG
CGACTAAGCGCTCAATCTGTCAAAGCACGATTGATTTTGTTGAGTTCGCCCTACAGTGGCAGTGGAGCCCTGAGCAGATAAGTGCCGTGGCAAAAGAACT
TGACCTATCGGTCAGCCACGAATGGATTTACCGGTATGTAACAGCTGACAAGGCTCGAGGTGGTGATTTGTATACCCATTTGAGGCAAGGCAAGCGGCGC
TACCGTAAGGGGTATGGGCAGAAGCGAGGCGGTATCCCTGATGCCATATCGATAGAGCTGCGCCCGGCGGTCGTTGATGCTCGTGAGCGGCTGGGAGACT
GGGAGGCTGATTTGGTACTGGGTAAACAGGGTAGCGGTGCCATCGTGACCTTGGCGGAGCGAAAGAGCCGTCTCTATCTGACGAAAAAGGTGTTTTCGAA
AGAGGCCCCAGAGGTGAGCAACGCCATCATCTCGCTGCTCCGTGACTACAAAGATGTGTGTCACACGATAACCTTCGATAACGGACTGGAGTTCAGTGAG
CACAAGGAGATCGCAAAGGCGCTGGACGCTGAAATGTACTTTGCCCACCCCTATTCTTCACATGAAAGGGGGTTAAATGAGAACATAAATGGGCTTCTGC
GGCAGTATATCCCGAAGGGAACTGACCTTAGAGAGGTGTCAGAAGAGGACTTACAGCGCTATCAGGGAGCCCTGAACAGCAGGCCACGAAAGTGCCTGGG
ATACAGGCAGCCCTCGGTGGTGTTTGCGGAGCTAAGAAAGGCGGCTTAGCGTTGCACTTCGGAGTTGAATCCGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
948 bp315 aa1021049+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKYQQLTAGLRYQIALLYGESFSCREIGLRVGVSKSTVSRELRRNSTPNGYAPDEAQRLSELRRSGATKRSICQSTIDFVEFALQWQWSPEQISAVAKEL
DLSVSHEWIYRYVTADKARGGDLYTHLRQGKRRYRKGYGQKRGGIPDAISIELRPAVVDARERLGDWEADLVLGKQGSGAIVTLAERKSRLYLTKKVFSK
EAPEVSNAIISLLRDYKDVCHTITFDNGLEFSEHKEIAKALDAEMYFAHPYSSHERGLNENINGLLRQYIPKGTDLREVSEEDLQRYQGALNSRPRKCLG
YRQPSVVFAELRKAA

 

Blast result :
Comments
ISAeme18 is 81% aa similar to ISSba17.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694