ISAeme17

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1941 bp

Ends


IR Length : 23/29

IRL : TGTCACCGCCGATGTAAAAATGACCCACTAACGCCGGAGTAAAGTTGACC
IRR : TGTCAACGCCGACCGAAAACTGATCCACTCTGGCTAATTTCGCCGATTGA

Insertion site


Left flankDirect repeatRight flankDR Length
GTAGACCAGTCAATAGCTGA0

DNA sequence

TGTCACCGCCGATGTAAAAATGACCCACTAACGCCGGAGTAAAGTTGACCCACCTAGGCCAAGATCGTGGCTTTTGAGCCACGCCCATGTTGACCCAGGA
GTTATCAGTGGAAATCAGAGTGTTGGCCCGTCAGGGCATGAGCATCCGGGCTATCGCCCAGCAATTGCGGGTGTCCCGTAACACGGTTCGCAAGTATTTG
CGCAACCCGGGCCTGCCAATCTATCCTAGCCGAGCTGTCCGACCAACCAAGCTCGATCCTTTTAAACCCTATCTGCAAGCGCGTATCCAGGCAGCGAAGC
CACACTGGATCCCGGCGACGGTTCTGCTCAGGGAACTGCGAGAGCATGGTTACACTGGCGGGCTCTCCCAGCTCAAGGCCTGGTTATCTCCTCTGCGCCA
TGACGCCTCTGGCACTGTCGTCCGTTTTGAAACGGCCCCCGGTAAACAGATGCAGGTCGATTTCACCATTATCCGCCGCGGCAATCGGCCTCTGAAGGCC
TTTGTTGCTACCCTGGGATTTAGTCGCGCCAGCTATGTCCGTTTCTTCGATCACGAACGTAATGATGCCTGGTTGACCGGACTGCGTGAGGCTTGTCATT
TCTTCGGTGGGGTCCCGCAGGAGGTGCTATTTGATAATGCCAGCACCATCATCCGCGAGCGGGATGCCTATGGTGAAGGTGACCATCGTTGGCACCCGGC
CCTGCTCGCCTTGGCCGAAGAGTTCGGTTTTCGCCCCCGCGTCTGCCGTCCCTACCGGGCCCAGACCAAAGGCAAGGTCGAGCGCTTCAACGGCTACCTC
AAGCGCAGTTTTGTCACACCACTGGCAGCCACCCTCAAGCAGGCAGGACTGGCGCTAGATGTGTCAACCGCCAATGCCCATATCGGCCCCTGGTTGCAAG
ACATCGCCAACCAGCGTAGCCATGGCACTACCGGTGAAGTCCCGGCTATCCGATTGCAACAAGAGCTGCCTCATTTTCTACCCCTGCCACAGGCGCTACC
CGTTAGTCCCGGCACCACGGCCACTGCGCGCCCCATGCCGCTGGAGAGCTTGCAGCATCCGCTATCGGTATACCAAGACCTGCTGGAGGTGAACGTATGA
ACTTGCAGCATGAACGCCTGATGGCCCTGAGTCAGACGCTGCGCTTGGAAGGGATTGCTGCCCAGTGGTCAGCACTAGCTCAGAAGGCGGCCAACGAGGA
GGCGAGCTTCGCCGATTATCTGGAGTGGTTGTTGCACGCAGAGCAGACTTTGAGGACCGAGCGCACCCGCCAGACCTTGCTCAAACTGGCCACTTTACCC
GTGGTCAAAACGCTGGAGCAGTACGACTTCGGCTTTGCCAGTGGCGCACCTCGCAGCCAGTTGCAGGAGTTAGCCAGTCTGGCCTTTATCGAGCGTAGCG
AAAACATAGTGCTGCTGGGCCCAAGCGGAGTGGGCAAGACGCATCTTGCCATCGCACTGGCCTATCGGGCTCTGATGGCTGGCATCAAGACCAGATTTAT
CAGCGCAGCAGACCTGATGTTGCAGCTGCAAGCAGCTCAACGACAGGAGCGGCTTAATGCCTACTTCAGCCGTGTCGTGATGGGGCCGCGTTTACTGGTG
ATCGATGAGATAGGCTACTTGCCGTTTGGGCGTGAGGAAGCAAACCTGTTTTTCAATGTGATCGCCAAACGTTATGAGAAAGGGAGCCTGATCTTGACGA
GCAATCTGCCCTTTAGCCAGTGGGCCAGCACCTTTGCCGATGATCAGACACTGACGGCAGCTATGCTCGATAGGCTGTTACATCATGCACACATCGTTCA
GATCAGCGGTGAAAGCTATCGTTTGAAAGAGAAACGCAAAGCAGGGGCCACACCCAAAACCAATTAACCCCATGTCCGGGTGGATCACTTTTCAATCGGC
GAAATTAGCCAGAGTGGATCAGTTTTCGGTCGGCGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1014 bp337 aa871100+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTQELSVEIRVLARQGMSIRAIAQQLRVSRNTVRKYLRNPGLPIYPSRAVRPTKLDPFKPYLQARIQAAKPHWIPATVLLRELREHGYTGGLSQLKAWL
SPLRHDASGTVVRFETAPGKQMQVDFTIIRRGNRPLKAFVATLGFSRASYVRFFDHERNDAWLTGLREACHFFGGVPQEVLFDNASTIIRERDAYGEGDH
RWHPALLALAEEFGFRPRVCRPYRAQTKGKVERFNGYLKRSFVTPLAATLKQAGLALDVSTANAHIGPWLQDIANQRSHGTTGEVPAIRLQQELPHFLPL
PQALPVSPGTTATARPMPLESLQHPLSVYQDLLEVNV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
762 bp253 aa10971858+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNLQHERLMALSQTLRLEGIAAQWSALAQKAANEEASFADYLEWLLHAEQTLRTERTRQTLLKLATLPVVKTLEQYDFGFASGAPRSQLQELASLAFIER
SENIVLLGPSGVGKTHLAIALAYRALMAGIKTRFISAADLMLQLQAAQRQERLNAYFSRVVMGPRLLVIDEIGYLPFGREEANLFFNVIAKRYEKGSLIL
TSNLPFSQWASTFADDQTLTAAMLDRLLHHAHIVQISGESYRLKEKRKAGATP

 

Blast result :
Comments
ISAema17 is 78%(istA, the transposase) aa similar to ISPsy4 and 87%(istB, transposition helper) to ISRso19.
This element is targetted by two transposons (ISApu2 and IS5) in the genome.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694