ISAve1

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP002607 ND Aeromonas veronii
Aeromonas veronii B565
DNA section
IS Length : 1393 bp

Ends


IR Length : 11

IRL : taatggaATGGTCGCCCCTCCCCAAAATGGCATCTAACGTGCCAAAGTGG
IRR : ----tagATGGTCGCCCCGGTTTGCCAAGCCTTCAATTGATGACGGTGAG

Insertion site


Left flankDirect repeatRight flankDR Length
ATAAATAAACTGAATGCCCT0
ATAAATAAACTGAATACCTT0
ATAAATAAACTGAATACCCT0

DNA sequence

TAATGGAATGGTCGCCCCTCCCCAAAATGGCATCTAACGTGCCAAAGTGGCGAATGAAACTTCCCACTGATTGAGAGAGGCGGCCGTGATGAACATTACA
ACGATTGGTATCGATCTGGCAAAATCCTGCATCCAAGTCCATTGCGTCGATGTGCACGGCAAGCGGATCCTCAACAAGAAACTCAAACGCGAGCAGGTGG
TGCCGTTCTTCAGCAACCGACCCGCCTGTCTGATTGGCATGGAGGCCTGCGGCAGCGCCCACTTCTGGGCTAACAAACTTCAGGCGCTGGGGCATACCGT
CAAGCTGATGGCACCGCAGTTCGTCAAACCTTACGTCAAAACCAACAAGAACGATGCGGCCGATGCCGAAGCCATTTGCGAGGCGGTGAGCCGCCCGAAC
ATGCGCTTCGTGCCGATTAAAAACAGCGAACAACTGGCCGTCCTGGCAATGCATCGGGCCCGCCAGGGATTCGTCAAGGCGAGGACCGCGCAGGCAAACC
AACTCCGGGGGCTGCTAGCGGAGCACGGTATTATCATCCCCAAGGGCATTCGCCACATCAATCAGCACCTGCCTGACATTCTGGAGGCGGGGGATAATGG
CTTGCCAGGGCGGTTTCGTCAGTTACTCGACCGCTTGAGCGCTCACTTCAAGGAGCTCGATAAGCAGGTGAGGGAATTGGACGTTCAGCTCCAAAGTTGG
CATCAGGACAGTCCGGCCAGCCAGAAACTGGCGAAGATACCGGGTATCGGTCCACTCACAGCCAGCGCGCTGGTTGCTACCATCGGCGATGCCAAACAGT
TTCAGAACGGCCGGCAGTTGGCAGCCTGGCTGGGGTTGGTGCCCAGGCAACACTCCAGCGGCGGCAAGCCAACACTGCTGGGGATAAGCAAGCGCGGCGA
TACTTATCTGCGCACCCTACTCATCCATGGCGCCCGCGCCGTGCTGCGGGTGGTGAAGAGCAAGGCACAACACGCAGGTAGTTGGCTGGAGGGGGTTATG
GCGCGGCGCAATCATAATGTGGCGGCGGTAGCACAGGCCAACAAGACGGCCCGCATCGTCTGGGCTCTACTAGCAAATGACCGAGAATTTGATGCGCAAT
ACAGCACAGGCACAATCCTAGCGGCGTAATGACAGGACAATAGCAAAGGCAGAAAAAGAACCACCGATTGCACAGGCAATCATGACGTGATGGCGAGACA
GGTCAGACCGTGACTGGCAAAACCCGAGGTATTCAAAGCACTTTCGAGAGCGACAAACTGATAGAGGAGCCAGTCAGCGAATTCCATCAGGGACAGAGGC
AGTAGCCTCGATCACAGTCCGGATCTATGGCTGCAATCTCAACCTAACTCACCGTCATCAATTGAAGGCTTGGCAAACCGGGGCGACCATCTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1041 bp346 aa891129+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MNITTIGIDLAKSCIQVHCVDVHGKRILNKKLKREQVVPFFSNRPACLIGMEACGSAHFWANKLQALGHTVKLMAPQFVKPYVKTNKNDAADAEAICEAV
SRPNMRFVPIKNSEQLAVLAMHRARQGFVKARTAQANQLRGLLAEHGIIIPKGIRHINQHLPDILEAGDNGLPGRFRQLLDRLSAHFKELDKQVRELDVQ
LQSWHQDSPASQKLAKIPGIGPLTASALVATIGDAKQFQNGRQLAAWLGLVPRQHSSGGKPTLLGISKRGDTYLRTLLIHGARAVLRVVKSKAQHAGSWL
EGVMARRNHNVAAVAQANKTARIVWALLANDREFDAQYSTGTILAA

 

Blast result :
Comments
ISAve1 is 85% aa similar to ISAs16.
The insertion sites are highly similar, belonging to transposon ISAve2 (one copy) or an element with 90% seq_id to ISAve2 but with a frameshift (two copies).
References
1] Pfeiffer F. (2015) Direct submission
2] Li Y., Liu Y., Zhou Z., Huang H., Ren Y., Zhang Y., Li G., Zhou Z. and Wang,L. (2011) J. Bacteriol. 193 (13), 3389-3390