ISAeme16

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1162 bp

Ends


IR Length : 11/15

IRL : AATAACCGTTCCGAAATGTAGATCACTTATCCGGGAAAGAAGGGAACTCG
IRR : AATAGCTGATCCTAATTTCGCTCCAGTTGCATAAGCCCATGTCCATTACC

Insertion site


Left flankDirect repeatRight flankDR Length
CCCCCAACCCTAGATTGAGCAG2

DNA sequence

AATAACCGTTCCGAAATGTAGATCACTTATCCGGGAAAGAAGGGAACTCGGCGTCGTTTGTGCGATCTGATTAATCGCCAAACCAATCAAACGACAAACG
AGCCGAGCCCGAAATGATACTAACACTCCCCGACCATGATGAGCGACGCCGGATCATCAACAAGATGCACCGGACCAAAGATAAAGATCTCTGCCGGAGG
CTCAACGCCATCTTGCTCCTTGCTGACGGGCACAGTGTCTCGGCTGTTTCTCGGCTCACAGCCGCCGCCCGGTCATCGATCAATCGCTGGGTGAACTGGT
ACACCTTGTACGGTCTGGAGGGGCTTGAAAGCGAGCCTCGGGGGCGCAAGCCGGTGCTGCCATTTGCTCACATCTCGGGTCTATTACAGCTGTTGATCCA
GTTGTCTCCACAGGAGCTCGGATATCAGCGCTCGCGTTGGAGTACCGAACTGATGGCGCTTGAACTCAAACGGAGTTGGCGCCTGACATTGCATGCTTCA
ACAATACGGCGATGGCTGCCCAGGCTAGGGATTGTCTGGCGCCGGGCCGCGCCCACTTTGCACATCCGCGATCCCCATAAAGAGCGGAAAATGGCCGCCA
TCAATGAGGCCTTGGGACGCTGCAGTGCGGACCATCCCGTGTTCTATGAAGATGAGGTAGATATCCACCTAAATCCCAAGCTGGGGGCGGACTGGATGAT
GCGAGGGAAGCAGAAGAAAGTGGCAACTCCGGGCCAGAATCGCAAACATTACCTCGCTGGCGCCCTGCATGCCGGAACAGGTAACGTGACCTATGTGGCG
AGCGATAGCAAAGACACGGACTTGTTCTTGTCACTGCTCCAAACACTAAAGCGGACTTATCGGCGCGCCAAGAGCATCACGCTGATCCTGGATAACTACA
TCATCCACAAGAGCAAAAAAGCACAGCGATGGCTGGCAAAAAATCCGAAGTTCATCGTGCTTTTTCAGCCGGTGTATTCGCCATGGGTGAACCAGATTGA
GCGGCTGTGGCATGCCCTCCATGAAACGATCACCCGGAACCACCAATGCCGCACATTGGGTGAGTTATTACGTCGCGTGTTCTACTTCCTGGATCATGCC
GCCCCGTTCCCCGGTAATGGACATGGGCTTATGCAACTGGAGCGAAATTAGGATCAGCTATT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1038 bp345 aa1141151+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MILTLPDHDERRRIINKMHRTKDKDLCRRLNAILLLADGHSVSAVSRLTAAARSSINRWVNWYTLYGLEGLESEPRGRKPVLPFAHISGLLQLLIQLSPQ
ELGYQRSRWSTELMALELKRSWRLTLHASTIRRWLPRLGIVWRRAAPTLHIRDPHKERKMAAINEALGRCSADHPVFYEDEVDIHLNPKLGADWMMRGKQ
KKVATPGQNRKHYLAGALHAGTGNVTYVASDSKDTDLFLSLLQTLKRTYRRAKSITLILDNYIIHKSKKAQRWLAKNPKFIVLFQPVYSPWVNQIERLWH
ALHETITRNHQCRTLGELLRRVFYFLDHAAPFPGNGHGLMQLERN

 

Blast result :
Comments
ISAeme16 is 78% aa similar to ISSpu2.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694