ISAeme15

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1429 bp

Ends


IR Length : 16/18

IRL : CAATGCTGATCAGTTAAGGATCCATTGACCGATCCTTCTGCTGTAGGACA
IRR : CAATGCTGTTCACTTAAGCGGAGCCGCATTGCGATTTACCGGAAAACGGG

Insertion site


Left flankDirect repeatRight flankDR Length
ACAACGAGGCCACCCCAGGGGTGGCCTTTTGAT13
TAAAAAAGGCCACGTCAAGCGTGGCCTTTTTTT13
AGCAAAAAGCCCGCCATTTGGCGGGCTTTTTCA13
AGAAAAGCCCCGCCAATCTGGCGGGGCTTTTCT13
GTAAAAAGCCCGCCATGTCGGCGGGCTTTTTCA13
AAAGAAACCCCGGCATCAAGCCGGGGTTTTGTC13
TAGAAAGGGCCGGAACATGACCGGCCCTCTCTG13
TGAAAAAGCCCTCGCAGTGCGAGGGCTTTTGTT13
GCAATAAGGCCACCCCAGGGTGGCCTTATTGT12
TTTGAAAGCCCACCCCTTGGTGGGCTTTTTTG12
GCAAAGGGCTCCCGAATGGGAGCCCTTTTTGC12
AAAAAAACGCCCCGCAGTGCGGGGCGTTTTGG12
CAAAAAGGCCCCTTTGACAGGGGCCTTTTGTC12
ACAAAGACCCCGGCCTTGGCCGGGGTCTTTGT12
AACAAAACCCCGGCGGTGGGCCGGGGTTTGAT12
GCTGCAGCCCCGTCCATTGGGGGCTTTTTCAC12
AAAAAATGCCCGTCGATGACGGGCATTTTTGT12
TGAAAAAGCCGCCCCAGGGGGCGGCCTTGCCA12
ATGAAAAGGGGGCTCAGTGGCGAGCTCTTCCC12
GAGAAAGAGGTGCACAGGCGAGGCCATTTATT12
TAAAAAATGCCACCCTAGGGCGGCATTTTGC11
CAACAAACCCCAGCCTGGCTGGGGTTTGTCT11
AAAAAAAGGCCCACAGTCGTGGGCCTTTTTG11
TAAACAAGGCCCCATTATGGGGCCTTGTTGT11
AAAAGAAAACCCCGATTGTCGGGGCGTTTTG11
ATGAGAAAAGCCGCAGTGCGGGGCTTTTTGC11
GATGAAAAGACCGCCATGCGGGGTCTTTCGT11
ATGTCAAGAGCGCATACTGCGGGCTTTTTTT11
AAGAAACAGGCGCCATGTGCGGCCCTGTTTG11
AGACTAAAGCCGCCGATGCGGGGCTTTTTAT11
TGCCACAACGCGGCAAGCCGGGCGTTGTGGG11
AAACAAACGCCGGCTTGGCCGGGGCGTTTGT11
CAACAAAAAGCTCCATGATGGAGGCCTTCTT11
AGTAGACAACGGGCCATCCCGACCCTTTACC11
AATCAAAAAGCGCAATTGCGGCCTTTTTCT10
GCAACTGCGGGTGCCAGTAA0

DNA sequence

CAATGCTGATCAGTTAAGGATCCATTGACCGATCCTTCTGCTGTAGGACAATCCGGAACCAATCACTGGTTCCGGATTTTTTATGTCTATTGCAAACGAT
CTGAACGATGTTGTTGAGGCATCTGGCGACATGCTGGCTCGGCTGGCCATTTTTGCCGACCATATTCCCGACGAATGGATTACTGCCGCTGCCGCCTTGT
CTGAAAAGGCCACTATCCGCCGACGCCGCTTACCTTCTGATATGGTCTTGTGGCTCGTCGTGGGGATGGCCTTTTTCCGCAATGGGCCCATCTCCGAGGT
CGCTCGGCGCCTCAACATCTGTGCAGAAGGGTTGGCCAATGATGCCTTGCTCGCGGACAGTGCCCTGTCTCAGGCCCGTCAGCGACTCGGCAAACAGCCG
CTCGAGTGGCTGTTCAAACAGTGCGCCGATGTGTGGGGGCGGGAACGTTATCCTGAAGACCATTGGCACGGTTTACAGGTCTTTGCCATCGATGGGGCCC
TGTTCCGAACCCAGGATATGCCTGAACTCAGAGCACATTTCGGCTCGGGTAACACCTCAACCAACCGGCAGACACCCTACCCGATGCTGCGGTTGGTCAC
GCTCATGAATGTCCGCTCGCATGTCATTGCCAACGCGGCCATCAGCCCGTACCGCAAAGGGGAAATCCCCCTGGCCAGCGAGTTCATTCACTCATTGCCG
GACAAGTCAGTGACCCTGCTGGATAAAGGCTTCTTCGGTGCCGACCTGTTGCTGCGTATTCAGGCCGAAGACACCGACCGTCACTGGCTCATCCCGGAAC
GCAAGGGGCTGGTATACACGGAACTTGAGCGCTATGGCGACGGTGACCGTCTGTTGCAAATGACGGTCTCGCCTCAGGCTCGCAAGAAAAACCCGGCGTT
GCCGACACACTGGCAAGTTCGCGCAGTGACCTATGAGGTGGCAGGCAAGGAGAAAACGGTCTTTACCTCCCTGCCCGTGGCGCGATTCAGCGCAGCTCAG
GTGGCGACCCTGTACCACGAGCGCTGGGAAATCGAGCTGGGATACCGGGATATCAAAAGCGCGATGCAGCACAACGCCATCACGCTGAGAAGCAAGAAAG
TGGACTTGGTCTATCAGGAGCTGTGGGGATTGCTGCTTGGATACAATCTGGTCAGGCGGGAGGCCAGCCAGGCCGCCGTAGCCCATCAGCGAGCGCCCAA
TGAGGTGAGCTTTAAGTTTGCCTGTCAGTTCATCGCGAACCAGATGGCAGTGATGGCGGGCGCGTTATCGCCAGCCCATACGCCACGGAGGTTGGCGGAG
TTGCGGGGCTGCATAGGCGTCATGTTCATAGAAAAACGCCCCAGGCCATCGAGACCCAGGGCGGTGAAGATATCAAAGACCCGTTTTCCGGTAAATCGCA
ATGCGGCTCCGCTTAAGTGAACAGCATTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1338 bp445 aa831420+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSIANDLNDVVEASGDMLARLAIFADHIPDEWITAAAALSEKATIRRRRLPSDMVLWLVVGMAFFRNGPISEVARRLNICAEGLANDALLADSALSQARQ
RLGKQPLEWLFKQCADVWGRERYPEDHWHGLQVFAIDGALFRTQDMPELRAHFGSGNTSTNRQTPYPMLRLVTLMNVRSHVIANAAISPYRKGEIPLASE
FIHSLPDKSVTLLDKGFFGADLLLRIQAEDTDRHWLIPERKGLVYTELERYGDGDRLLQMTVSPQARKKNPALPTHWQVRAVTYEVAGKEKTVFTSLPVA
RFSAAQVATLYHERWEIELGYRDIKSAMQHNAITLRSKKVDLVYQELWGLLLGYNLVRREASQAAVAHQRAPNEVSFKFACQFIANQMAVMAGALSPAHT
PRRLAELRGCIGVMFIEKRPRPSRPRAVKISKTRFPVNRNAAPLK

 

Blast result :
Comments
ISAeme15 is 82% aa similar to ISVvu3.
IS_pep_1 is B224_0435
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694