ISAeme14

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1425 bp

Ends


IR Length : 16/18

IRL : TAATGCCGATCAGTTAAGGATCCATTGACCGATCCGCCTGATATGCAAAA
IRR : TAATGCCAGTCAGTTAAGCCTGACTGGCATTGTTTTTACTGGCTTTTTCA

Insertion site


Left flankDirect repeatRight flankDR Length
AAAAAAGTCCCCGCAATGCGGGGACTTTTCAC12

DNA sequence

TAATGCCGATCAGTTAAGGATCCATTGACCGATCCGCCTGATATGCAAAAATCCGAAACCAGTTGATTTGGTTTCGGATTTTTTATGCGTATCGAACAGG
CTCTCGACTTCCTTCACGCCAGCAATGCTGCTCAGTTTGACTCACTCTCGGACCTCATTCCTCCCGAGCTCATTACCGTCCTGCTCCACGAAGAAGGGGT
CGTTACTCTGCGCCGTCGCCGCATGCCCATGGAGCGGCTGGTCTGGGCCATCATCGGCATGGCCATGTTCCGTCACGTTCCCATGACTCAGCTCGTCAAT
CAGCTCGATATCCTGCTCCCGGGCGAGCGCCCCTTCGTCGTCCCCAGCGCCTTTCTGCAAGCCCGCCAGAAGCTCGGGGATAAAAGCATCGAGCGCCTGT
TCCACGAGACCACTGCCCGTTGGTATCAACCGTCTCAGCACCCGGATTGGGCCGGCCTGCAACTGCTGGCCGTCGATGGGGTGATGTGGCGTACCCCCGA
CACCGCCGAGAACACGGCGGCCTTCGCCAAACCCAAGACCCAGCAGGGCGAGACGGCTTATCCGCAAGTACGCATGCTTTGCCAGATGGAGCTGACCAGC
CATCTGCTCACCCAGGCCGTGATGGAGAGCTGCGCCGTCAGCGAGATGGTGCTGGCAGAGCAGCTTGTTGAACGCACACCGGACCACTCACTGACCCTGT
TCGACAAGGGGTTCTACTCGCTCGGGCTGCTCCATGCCTGGCAGACGACGGGCCGTGAGCGCCACTGGCTACTGCCGCTCAAGAAGGGCACTCAGTACGA
GGTAGTCCGCAAGCTGGGGCGCCAGGATGCGCTGGTGCAGCTCAAGACCAGTCCACAGGCGAGGAAGAAATGGCCGCAACTACCCGAGACGATGGTGGCC
CGACTGCTGACCCGAACCATCAACGGCAAGGCGCGGCAGGTGCTGACCTCGATGGTCGACCCGATGCGCTTCCCGGGTGCGGACATCATCGCGCTCTATG
GTCATCGCTGGGAAATCGAGCTGGGGTATCGGGAGATGAAACACAGCCTGCAACAGCACAAGCTGACCCTGCGCAGCAAGAAGGCGGCGGGCATACGGCA
AGAGTTGTGGGGCGTGCTGCTGGCGTACAACCTGCTACGCAGTCAGATGGTGAAGATGGCGGCAAGTCTGAAGGGATACACGGCGAGCCAACTGAGTTTT
CACATGGCGTCGGTGTACCTCATTCATGAACTGAGCGCGATGCCGTATGTGTCGCCGGGCAACATCCCGGGGCGAGTGGCCGAGCTGGAGCAGCAGGCGG
GGCAGTTCATTTTGCCTCAGCGAAGGGAGCGAAGTTATCCGCGTTGCGTGAAGGTAAGGCCCCAGAAGTATGCGGTGAAAAAGCCAGTAAAAACAATGCC
AGTCAGGCTTAACTGACTGGCATTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1332 bp443 aa851416+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRIEQALDFLHASNAAQFDSLSDLIPPELITVLLHEEGVVTLRRRRMPMERLVWAIIGMAMFRHVPMTQLVNQLDILLPGERPFVVPSAFLQARQKLGDK
SIERLFHETTARWYQPSQHPDWAGLQLLAVDGVMWRTPDTAENTAAFAKPKTQQGETAYPQVRMLCQMELTSHLLTQAVMESCAVSEMVLAEQLVERTPD
HSLTLFDKGFYSLGLLHAWQTTGRERHWLLPLKKGTQYEVVRKLGRQDALVQLKTSPQARKKWPQLPETMVARLLTRTINGKARQVLTSMVDPMRFPGAD
IIALYGHRWEIELGYREMKHSLQQHKLTLRSKKAAGIRQELWGVLLAYNLLRSQMVKMAASLKGYTASQLSFHMASVYLIHELSAMPYVSPGNIPGRVAE
LEQQAGQFILPQRRERSYPRCVKVRPQKYAVKKPVKTMPVRLN

 

Blast result :
Comments
ISAeme14 is 91% aa similar to ISAs30.
IS_pep_1 is B224_4040.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694