ISAeme13

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1425 bp

Ends


IR Length : 16/18

IRL : TAATGCCGATCAGTTAAGGATCCATTGACCGATCCTTCTGTTGTGTGAAA
IRR : TAATGCCAGTCAGTTAAGCCTGACTGGCATTGTTTTTATTGGCTTTTTTC

Insertion site


Left flankDirect repeatRight flankDR Length
AAAATGAGGGAGGCATGATGCCTCCCTCTTTC12

DNA sequence

TAATGCCGATCAGTTAAGGATCCATTGACCGATCCTTCTGTTGTGTGAAAATCCGAAACCTGTTGATTGGTTTCGGATTTTTTATGCGTATCGCTCACGC
TCTCGACTTCCTCCACGCCAGCAATGCTGCCCAGTTTGAGTCACTCTCAGACCTCATTGCTCCTGAGCTCATCACCACTCTGCTCGCCGAAGAGGATGTC
GCTACCCTGCGTCGCCGCCGTATGCCCATGGAGCGTCTGGTCTGGGCCATTATCGGCATGGCTATCTTCCGCCACGTCCCCATGACCCAGCTGGTCAATC
AGCTTGATATCCTGTTGCCCGGCGACCGCCCCTTCGTCGCCCCCAGTGCCTTCCTGCAAGCTCGACAAAAACTGGGAGACAAGAGCATCGAACGCCTGTT
TCACGAGACCGCTTCGCGCTGGCACCAGCAGGCCAATCATCCGGGTTGGGCCGGGCTGCAACTGCTCGCCGTTGATGGCGTGATGTGGCGCACACCCGAT
ACCCCGGACAATGCCGCCGAGTTTGCCAAACCCGGCACCCAGCATGGTGAGACCACCTATCCGCAGGTGCGCATGCTCTGCCAGATGGAGCTGACCAGCC
ACCTGCTTGTTCAGGCCGTCATGGAGAGCTGCTCCGTCAACGAGATGGTGTTGGCCGAGCAACTCATCGCGCGTACACCGGATCACTCGCTCACCCTGTT
CGACAAGGGGTTTTACTCGCTCGGTTTGCTCCACGCCTGGCAAACCACTGGCAGCGAGCGCCACTGGCTGCTGCCACTCAAAAAGGGCACGCAATACGAA
GTGGTGCGCAAGCTGGGGCGTCAGGATGTGCTAGTGCGTCTCAAAACCAGTCCACAGGCGAGGAAGAAGTGGCCGCACCTGCCAGAGACACTAGAAGCCC
GCTTACTGACCCGCACCATCAACGGCAAAGAGCGGCAAGTGCTGACCTCGATGGTCGACCCGATGCGCTTTGTGGGCGCCGATATCGTCGACTTGTATGG
TCATCGTTGGGAAATCGAGTTGGGCTATCGGGAGATGAAGCACAGCCTGCAACAGCACCGGCTGACCCTGCGCAGCAAGAAGGCGGCGGGGATACGGCAA
GAGCTATGGGGTGTGTTGTTGGCGTACAACCTGCTGCGCAGCCAGATGGTGAAGATGGCGGCAAGTCTGAAGGGATACACGGCGAGCCAGCTGAGTTTTC
ATATGGCATCGGTGTACCTCATCCATGAACTGAGCTGCATGCCGTACCTATCACCGGGGAGTATCCCGAAGCGTGTGGCGGAGCTGGATAAGCAGGCGGG
GCAGTTTGTATTGCCGGAGCGAAGGGAGCGGAGTTACCCCCGCAGTGTGAAGGCAAGACCACAGAGGTATAGTGTGAAAAAAGCCAATAAAAACAATGCC
AGTCAGGCTTAACTGACTGGCATTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1329 bp442 aa841412+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRIAHALDFLHASNAAQFESLSDLIAPELITTLLAEEDVATLRRRRMPMERLVWAIIGMAIFRHVPMTQLVNQLDILLPGDRPFVAPSAFLQARQKLGDK
SIERLFHETASRWHQQANHPGWAGLQLLAVDGVMWRTPDTPDNAAEFAKPGTQHGETTYPQVRMLCQMELTSHLLVQAVMESCSVNEMVLAEQLIARTPD
HSLTLFDKGFYSLGLLHAWQTTGSERHWLLPLKKGTQYEVVRKLGRQDVLVRLKTSPQARKKWPHLPETLEARLLTRTINGKERQVLTSMVDPMRFVGAD
IVDLYGHRWEIELGYREMKHSLQQHRLTLRSKKAAGIRQELWGVLLAYNLLRSQMVKMAASLKGYTASQLSFHMASVYLIHELSCMPYLSPGSIPKRVAE
LDKQAGQFVLPERRERSYPRSVKARPQRYSVKKANKNNASQA

 

Blast result :
Comments
ISAeme13 is 95% aa similar to ISAs30.
IS_pep_1 is B224_3414.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694