ISAeme11

  • Family IS701
  • Group ISAba11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1108 bp

Ends


IR Length : 16

IRL : CTGAAGTTACGCACTCAACTGCTTCCGGCATAATGACGGCATGAACCAAG
IRR : CTGAAGTTACGCACTCCCCCTGAGCACCCGCAATTCGTCAAACGCTATTC

Insertion site


Left flankDirect repeatRight flankDR Length
GCTCTCTCTATTCGTAACCTCGATCAC7

DNA sequence

CTGAAGTTACGCACTCAACTGCTTCCGGCATAATGACGGCATGAACCAAGAACGCCTCGACCTTTACTCTGACTACCTCTGTGCCACCTTTGGCAAGGCC
ACTGCCACCGGCTTGGCCGCTATGCTGGACGACAGCATTAGTCATGACGCTATAACCCGCTTTCTCGCCGGACCTGAGCTCACTTCCCGTGACCTCTGGC
GCCAAGTCAAACCCTTGGTGCGCCAAATCGAAGCCCATGACGGCGTGCTCATTTTCGATGACACCGTGCAAGCCAAGCCCCATATGGCCGAAAATGACCT
CATCGCCTGGCACTTTGACCACACCCAAGGCCGCGCTGTCAAAGGCATCAATCTACTCAACTGCCTCTATCACGCGGGCGGCGTGTCGCTCCCCGTGGCC
TTTGAACTGGTGCGTAAGGATCTCCGTTTTTGCGATCTGCAGACCCGCCAGGAGCGGCGCGTCAGCAATGTGACCAAAAATGAGCAAATGCGCGCGATGC
TGGATGCCTGCCTCCATAATCAGCTGACGTTTCACTGTGTATTGTTTGATAGCTGGTTCAGTTCGTCCGACAACATGAAGCATATCAAGCAAGTGCGTCG
CAAGGATTTCATTGGTGCCCTCAAGAGCAACCGGTTGGTGGCGTTAAGTGAAGCCGACCGCAAGCAGCGTCGTTATACCCGCGTAGACCAACTCGATTGG
TCAGAACAAACCGCTATCACCGGCTGGCTGAAGGATGTGGATTTCCCGATACGGCTGGCCCGGCAAGTCTTTAAAAACAAGGACGGCAGCAGCGGTATTT
TGTATCTGGCCGGTAGTCAATTGGATGCTACCTGGAACGACCTCACTTCGACCTACCAGAAACGGTGGCAAGTCGAAGTGTTTCACAAATCATTGAAATC
CAACGCTGCCTTTGCCAAATCGCCAGACCATGGTGTTCGCACGCAAGCCAACCACTTGTTTGCCTCGATAGTGGCGGTGACCAAGCTGGAGGCCCTGAAA
ATCCGCACCAAGCTCAACCACTTTGCTCTGCGCGCCAAGCTCTATCTCAAAGCCACCCGAATAGCGTTTGACGAATTGCGGGTGCTCAGGGGGAGTGCGT
AACTTCAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1062 bp353 aa411102+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNQERLDLYSDYLCATFGKATATGLAAMLDDSISHDAITRFLAGPELTSRDLWRQVKPLVRQIEAHDGVLIFDDTVQAKPHMAENDLIAWHFDHTQGRAV
KGINLLNCLYHAGGVSLPVAFELVRKDLRFCDLQTRQERRVSNVTKNEQMRAMLDACLHNQLTFHCVLFDSWFSSSDNMKHIKQVRRKDFIGALKSNRLV
ALSEADRKQRRYTRVDQLDWSEQTAITGWLKDVDFPIRLARQVFKNKDGSSGILYLAGSQLDATWNDLTSTYQKRWQVEVFHKSLKSNAAFAKSPDHGVR
TQANHLFASIVAVTKLEALKIRTKLNHFALRAKLYLKATRIAFDELRVLRGSA

 

Blast result :
Comments
ISAeme11 is 50% aa similar to ISCysp20.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694