ISAeme9

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1332 bp

Ends


Left end : CAGATGCCCCGTAAACCCTCGCCCAATCGGGCGGGGATATAAGGGGCGGTAACTCAATGTTTTCTATTGAGTGGTAATATTTTTACTGTATATAATTACA II struct. : Yes
Right end : CTCGTCGTTTCTACCCATGGAGGCCAGCGTCAATCTGCCAATTCGGCAGTAGCGGCCTAAGAAGTGGGAAGCCTCGCCGCTTGCGGCGGGGAGCAGTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GCAGAAGCGAAGTTAGGAGACTGACATGtcac
TGCTGTTCATTTTTAGTGAACGGCTAGGtcac
TCCCATTTATCGTTAGCTTTCATCCGGCtcac
AGCAGAACATGCTTAGTCTCTTCAGGCCtcac

DNA sequence

CAGATGCCCCGTAAACCCTCGCCCAATCGGGCGGGGATATAAGGGGCGGTAACTCAATGTTTTCTATTGAGTGGTAATATTTTTACTGTATATAATTACA
GTATCTCGACATTTAAGGTTTGCCGGTGAAAAGAGCCACAAAAGTACGGATTTACCCCACTGACGAACAGGCGGCATTTTTGAATGCTCAGTTCGGCGCG
GTGCGGTTCGCGTACAACAAGACCCTTCATATTCAAAGCCACATGTTCAAGCGTCACGGGGTCTCACTGAAACCGAAACGCGACCTGAAGCCCTTGCTGG
CAGTGGCAAAAAAGTCGCGCAAATACGGCTGGCTGAAAGAGTACGATTCACTCGCCTTGCAGCAGGCGGTAATCAATCTGGACAAGGCGTTTGTCAACTT
CTTCAACCCCAAGCTCAAGGCGCGAATGCCAACGTTCAAGAGCAAACGGGGCAGGCAATCGAGCTATCACCCCAATGGCAAAGTGCTGGCCGACGCTATC
CAGTTACCGAAGATGACGCCCATCAGGGCGGTCATTCACCGAGACATAATCGGCGTGGTCTCCAGTATCACAGTCAGCCGTGGCCCGACAGGGAAATACT
ATGCGTCCATCCTTTGCGATGATGGCTGCGAGGCCCCGGCCAAGCCCACCTTCATCACCGACGTGACCGGCTGTGATATGGGGCTATCCCACTATCTCAT
CCAGTCCAACGGAAAGAAAACGGATAACCCGCGCCACCTTGTCAATGCCAGCCGCAATCTACGGCGCAAGCAAAAGGCACTGTCTCGCAAGAAAAAGGGC
AGCGCCAACCGTGGCAAGGCAAGGCGGCAACTGGCAGCCCTGCACGAACGGGTAGCCAATGCCCGCGCCGATTTTCAGCACAAACTCTCCCGCACGATGG
TTGACGATAGTCAAGCGATCATCGTGGAGACATTGAAATCAGCGAACATGATGAAAAACCACAAACTGGCCCGCGCCATAGGCGATGCTGGCTGGCATGG
TTTCATCATGAAGCTGGAGTACAAGGCTCAAGCGGCGGGTCGCCACCTAATCAAACTCGATCAGTGGTTTGCCAGCTCGAAACTGTGTAGCGATTGCGGC
CACAAGATGCCGGATATGCCGCTGCACCAGCGGCAGTGGGCATGTCCGGCGTGTGATGCAGAGCATGACCGCGACATCAACGCAGCCATGAATATCCAGC
AGCAAGGCATATTGGAATTAAAGGCGGCGGGACTCGTCGTTTCTACCCATGGAGGCCAGCGTCAATCTGCCAATTCGGCAGTAGCGGCCTAAGAAGTGGG
AAGCCTCGCCGCTTGCGGCGGGGAGCAGTCAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1167 bp388 aa1261292+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRATKVRIYPTDEQAAFLNAQFGAVRFAYNKTLHIQSHMFKRHGVSLKPKRDLKPLLAVAKKSRKYGWLKEYDSLALQQAVINLDKAFVNFFNPKLKAR
MPTFKSKRGRQSSYHPNGKVLADAIQLPKMTPIRAVIHRDIIGVVSSITVSRGPTGKYYASILCDDGCEAPAKPTFITDVTGCDMGLSHYLIQSNGKKTD
NPRHLVNASRNLRRKQKALSRKKKGSANRGKARRQLAALHERVANARADFQHKLSRTMVDDSQAIIVETLKSANMMKNHKLARAIGDAGWHGFIMKLEYK
AQAAGRHLIKLDQWFASSKLCSDCGHKMPDMPLHQRQWACPACDAEHDRDINAAMNIQQQGILELKAAGLVVSTHGGQRQSANSAVAA

 

Blast result :
Comments
ISAeme9 is 85% (TnpB) aa similar to ISEc46.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Chai, B., Wang, H., and Chen, X. (2012) J. Bacteriol. 194, 6693-6694