ISAeme8

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1739 bp

Ends


Left end : CAACTGCCCCGTAAACCCTCGCCCAATCGGGCGGGGATATAAGGGGAGCTAATCTGGTGTGTTCTGCGAGGCCACATACGCCTTGAGCGTTTCGATAGTG II struct. : No
Right end : CACGTCGTTTCTACCCATGGAGGCCAGCGTTAATCTGCCAATTCGGCAGTAGCGGCCTAAGAAGTGGGAAGCCTCGCCGCTTGCGGCGGGGAGCAGTCAC II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GCGCGCCGCTGGTTAGGCTATCTGGTTA
AAATCGATCCAGTTAGTGATGGAGGTTT
TGGACGCTGTTTTTAGGTGGCTGTGATA
CGCAGCATCTCGTTAGGCGAGGCTCCTG
GCGTGGCTGGGGTTAGTACCACGACAAC

DNA sequence

CAACTGCCCCGTAAACCCTCGCCCAATCGGGCGGGGATATAAGGGGAGCTAATCTGGTGTGTTCTGCGAGGCCACATACGCCTTGAGCGTTTCGATAGTG
GCCCCGCCAGCACTGCAAGCAAAGTAAGAGCGCGACCACAGGAGCCCGCTATTACTTTGCTTTCGTAGGTGCGTATTCAATATGCGGATCTGGCGGCTAC
TGATCGACTTCAAATTATTGACCAACACACTGACGGCCAACTTCGGTGGATAGGCTACGAGCAGATGAACGTGATCGGCTTCGCCATCCATCTCCAGAAT
GTCGATCTCCAACTTCTCTGCCGCAGATAAAAATGCGCCGCGCATCTGCTCGATCATGGTGCCATCAAGCAGTTTGCGGCGGTACTTCGTGGTGAAGATT
AAATGCACAACTAGCTTGCTGACGGAGTGACGCCTCCGCAGATACCTAGTCAAATCATCATGATGCACACTCACTTGCAATACTCCGTTAAAAGTCATAC
TGTATGGGTATCGCTAGCAGCGAGCGGTGACTTTGAGAAGAGCAACGAAAGTACGCATTTATCCGACCGACGAACAAGCGGCATTCTTGAATGCCCAGTT
CGGCGCGGTGCGGTTCGCGTACAACAAGGCCCTTCATATTCAACGACACATGTTCAAGCGTCACGGGATTTCACTGAAACCGAAACGCGACCTGAAACCC
ATGCTCGCAGTGGCGAAAAAATCGCGCAAATACGGCTGGCTAAAGGAGTACGATTCACTCGCCTTGCAGCAAGCGGTGATTAATCTCGACAAGGCATTCG
CCAACTTCTTCAATCCCAAGCTCAAGGCACGAATGCCTACCTTCAAGAGCAAACGGGGCAGGCAATCCAGCTATCACCCCAACGGAAAGGTGCTCACAGC
GGCTATCCAGTTGCCGAAGATGACGCCCATCAGGGCGGTCATTCATCGAGATATTATCGGCGTGGTATCCAGCATCACAGTCAGCCGTGGCCCGACAGGG
AAATACTATGCTTCCATCCTTTGCGAGGATGGCTGCGAGGCTCCCGCCAAGCCCACCTTCATCACGGACGTGACTGGCTGTGATATGGGGCTGTCCCACT
ACCTCATTGAGTCGAGCGGCAAAAAGATTGCCAATCCACGCCACCTTATCAACGCCAGTCGCAATCTACGACGCAAGCAAAAGGCACTGTCTCGCAAGAA
AAAGGGCAGCGCCAACCGTGGCAAGGCAAGACGGCAACTGGCCGCCCTGCACGAACGGGTAGCCAATGCCCGCGCCGATTTTCAGCACAAACTCTCTCAC
ACGATGGTTGACGAAAACCAAGCGGTCATCGTGGAGACATTGAAATCAGCGAACATGATGAAAAATCACAAACTGGCCCGCGCCATTGGCGATGCTGGTT
GGCATGGTTTCATCATGAAGCTGGAGTACAAGGCACAAGCGGCGGGTCGCCACCTAATCAAACTTGATCAGTGGTTTGCCAGCTCGAAACTGTGTAGCGA
TTGCGGCCACAAGATGCCGGACATGCCGTTGCATCAGCGGCAGTGGGTATGTCCAGCGTGTGGGGCAGGGCATGACCGCGACATTAACGCGGCCATGAAT
ATCCAGCAACAAGGCATATTGGAATTAAAGGCGGCGGGACACGTCGTTTCTACCCATGGAGGCCAGCGTTAATCTGCCAATTCGGCAGTAGCGGCCTAAG
AAGTGGGAAGCCTCGCCGCTTGCGGCGGGGAGCAGTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
426 bp141 aa47449-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MSVHHDDLTRYLRRRHSVSKLVVHLIFTTKYRRKLLDGTMIEQMRGAFLSAAEKLEIDILEMDGEADHVHLLVAYPPKLAVSVLVNNLKSISSRQIRILN
THLRKQSNSGLLWSRSYFACSAGGATIETLKAYVASQNTPD

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1146 bp381 aa5271672+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTLRRATKVRIYPTDEQAAFLNAQFGAVRFAYNKALHIQRHMFKRHGISLKPKRDLKPMLAVAKKSRKYGWLKEYDSLALQQAVINLDKAFANFFNPKLK
ARMPTFKSKRGRQSSYHPNGKVLTAAIQLPKMTPIRAVIHRDIIGVVSSITVSRGPTGKYYASILCEDGCEAPAKPTFITDVTGCDMGLSHYLIESSGKK
IANPRHLINASRNLRRKQKALSRKKKGSANRGKARRQLAALHERVANARADFQHKLSHTMVDENQAVIVETLKSANMMKNHKLARAIGDAGWHGFIMKLE
YKAQAAGRHLIKLDQWFASSKLCSDCGHKMPDMPLHQRQWVCPACGAGHDRDINAAMNIQQQGILELKAAGHVVSTHGGQR

 

Blast result :
Comments
ISAeme8 is 82% (TnpA : the transposase) and 87% (TnpB) aa similar to ISEc46.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Chai, B., Wang, H., and Chen, X. (2012) J. Bacteriol. 194, 6693-6694