ISAeme7

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1062 bp

Ends


IR Length : 18

IRL : GGCGTTGTTTCCCAAATCGATACCGCCTGAATGGAACTTACTGAGGGCGC
IRR : GGCGTTGTTTCCCAAATCAGCCATCAGGTCACAACCGCCCCAGCCCCAAG

Insertion site


Left flankDirect repeatRight flankDR Length
TAGTTTTCGGGCTTACAACATAGAAAGAA9

DNA sequence

GGCGTTGTTTCCCAAATCGATACCGCCTGAATGGAACTTACTGAGGGCGCGGTGATCTGATCACCACCACCCCCTTCAAGTCTGCATCATGGGCAAGTCG
CTTCACCCCATCACCAACTGGCCTAAATACAACAAGTCTTTGATTAACCGTGGCTCACTCACCTTCTGGGTTGATGCCGAGACCATGAACAACTGGTTTC
ACCACGACCATCATGGGCGGCGGGGCCGCAGCCAGCTTTACACTGACCAGACCATCTGTACCTTCCTGATGCTCAAGGGGATTTTCAACCTCACTCTGCG
GGCCACTCAGGGACTACTCGACTCCCTGTTCGAGTTGATGAACGTGCCGCTGTGCGCACCGGACTATAGCTGTGTCAGCAAGCGAGCACGCACGGTGACG
GTGGCTTATCGACAGCCCCCAAAGGGAGGCATTACCGACTTGGTTATCGACTCAACCGGGCTGAAGGTGTTTGGTGAAGGCGAGTGGAAAGTTAGAAAAC
ACGGCGCCGAGAAGCGGCGAGTCTGGCGCAAGTTACATCTGGCGGTAGACCCGGTGACCCACGATATCGTGGCGGCGGAAGTCTCGCTGGAAAATGTCCA
TGATGCCGAGGTGCTGCCGACTCTGCTCAATCCACTGCGGCGCAAGCTGGGACATGTTTATGCAGATGGTGCATATGACAGCAAAGCCAGCCATCAGCTT
ATCTCGCACAAAGGGGCGGCAGCCTGTATCCCGCCTCGCAAGAACGCGGGGTTATGGGAAAAGGGACATCCGAGAAATGAGGCGGTGCTGGTGATGCGCA
AGGAAGGGCTGGAGCACTGGAAGAAGATATCGGGTTATCACCGGCGCTCGCTGGCAGAGACGGCGATGTATCGGTTCAAGCAGTTGATGACGGGCAAAAT
CAGTCTGCGGACCTACAACGGTCAGGTAGGGGAAGTGATGGCGTATGTTGGTGCAATCAACAAACTGAACACCCTTGGTCTGCCTGTCAGAAAGCCCCGA
GTGTAACGGTCACTTGGGGCTGGGGCGGTTGTGACCTGATGGCTGATTTGGGAAACAACGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
918 bp305 aa891006+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGKSLHPITNWPKYNKSLINRGSLTFWVDAETMNNWFHHDHHGRRGRSQLYTDQTICTFLMLKGIFNLTLRATQGLLDSLFELMNVPLCAPDYSCVSKRA
RTVTVAYRQPPKGGITDLVIDSTGLKVFGEGEWKVRKHGAEKRRVWRKLHLAVDPVTHDIVAAEVSLENVHDAEVLPTLLNPLRRKLGHVYADGAYDSKA
SHQLISHKGAAACIPPRKNAGLWEKGHPRNEAVLVMRKEGLEHWKKISGYHRRSLAETAMYRFKQLMTGKISLRTYNGQVGEVMAYVGAINKLNTLGLPV
RKPRV

 

Blast result :
Comments
95% aa similar to ISAs14.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694