ISAeme4

  • Family IS4
  • Group ISH8
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1383 bp

Ends


IR Length : 16/20

IRL : CATGTACAGGATCTTTAGCAGCAACCAATTGAAATAGATATAAAAATGGC
IRR : CATATACAGGACCTTAAGAAAACACTTCATTTGAAATTCCTTCTAAGGTC

Insertion site


Left flankDirect repeatRight flankDR Length
CGTGCGGTGGCAAGGATGGACAAATGGGCATTATGCGCTGACAG0
ATGGCCCAGTTCAGTGCGAGCTAGGATGGACATCCCCTGTGCGC0

DNA sequence

CATGTACAGGATCTTTAGCAGCAACCAATTGAAATAGATATAAAAATGGCTTCGATTGTGATCTGATTTTATCGCCAAACAAAACCGCCACGCACGAAGC
CATGACCATTATCGATGCCAACCACTATCTTGAGCAACTTCTCTCTCCTGCCGAACTCGACCGCATTGCCCGTGACTGCCAATTTTGCTTGCGCAAACGA
GCCATTACGCCTGCCATGCTGGTGACTGCACTCTTGCGGGCACTTGGCTGCGACCAGGTCGATGGTATCGCTGGATTGCACCATCATTTCAATGCCTTGC
AACTGGTTGATGCCCATCAGGTTTCCTACAAGCCCTTTCACAACCAGTTGCGCAAAGCGTCGTTCGCCCTGTTCATGAAGGCGCTGGTTGAACGGGCTAT
CGCCTTGCGTATCGGCCAACAGATGAAGACTGCATCCCTTGGCGCATTCAAACAGGTGTTGTTGCAGGATGGTACTTCCTTTGCCGTCCACAAAAGCCTG
GCCGAAGTGTTTCCGGGAAGGTTCAAGACCATTAGCCCGGCGGCAATCGAGTGTCATATGCTCATGTCTCTATTGGAGCAAAGTCCAGTTTGCATGCAGG
TCTCTGCCGATACGGCGTCAGAGCGCCAGTTCCTGCCTGAGCCCGAGCGGCTCAATAACAGCCTGCTGTTGGCGGACGCGGGTTACATCGATATGGCCTA
CTTTGCGAAACTAAACGAGGCGGGCGGTTTCTATCTGGTCAGGGGTAGTAAGAGCCTGAACCCGAAGATTATCCGGGCATGGCGCGGTGACGAACGGGAA
GTGCCGAAGCTGGCGGGCCTGTCACTCAAGGATGCTGGACGGCGGCACTGTCGGGCAGAGGTACTGGATATGGTGGTGAAATCGGGCAAGTTCGAGTATC
GGCTGATCCGACGCTGGTTTGCCGAGGAGAAACGGTTCTGTATCTGGATGACCAATCTGCCACGAGAGGCATGGCCAGCAGAGCGGGTGATGAGCCTGTA
CCGCTGTCGTTGGCAGGTGGAGCTGCTGTTTAAGGAGTGGAAGTCGCACAACCGGCTCAAGGGCTTTGTGACAGGTCAGAAAGCCATTGCCGAGGGGCTG
GTGTGGGCCAGCCTGTTGTCGCTGGTGATGAAAAGACGGGTAGCTCAGACGCTGATGGGGGACGGTTTATCCACACTGAAAGCGGCGAAAAATAGCGGAA
CCTGGTGGTTCCCGTTATTGGAAGCGGTGGCTCATCGGGTGTTGGTGGAAATAAGAGAGCGTCTGGAATGGGCTGCCGTTTACTTGGCGAAAAATGCCCG
TCGAACCAAGCAGCGAAAATCGATACAGAACAGGACCTTAGAAGGAATTTCAAATGAAGTGTTTTCTTAAGGTCCTGTATATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1269 bp422 aa1021370+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTIIDANHYLEQLLSPAELDRIARDCQFCLRKRAITPAMLVTALLRALGCDQVDGIAGLHHHFNALQLVDAHQVSYKPFHNQLRKASFALFMKALVERAI
ALRIGQQMKTASLGAFKQVLLQDGTSFAVHKSLAEVFPGRFKTISPAAIECHMLMSLLEQSPVCMQVSADTASERQFLPEPERLNNSLLLADAGYIDMAY
FAKLNEAGGFYLVRGSKSLNPKIIRAWRGDEREVPKLAGLSLKDAGRRHCRAEVLDMVVKSGKFEYRLIRRWFAEEKRFCIWMTNLPREAWPAERVMSLY
RCRWQVELLFKEWKSHNRLKGFVTGQKAIAEGLVWASLLSLVMKRRVAQTLMGDGLSTLKAAKNSGTWWFPLLEAVAHRVLVEIRERLEWAAVYLAKNAR
RTKQRKSIQNRTLEGISNEVFS

 

Blast result :
Comments
ISAeme4 is 89% aa similar to ISApu1.
IS_pep_1 is B224_3136.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694