ISAeme3

  • Family IS4
  • Group ISH8
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1384 bp

Ends


IR Length : 18/20

IRL : CATGTACAGGATCTTTAGCGCCAACCAATTGAAATAGATATAAAAAAGGC
IRR : CATGTACAGGACCTTAAGCGTTGAGTCCCTTTAAAACTCCTTCCAAGGTC

Insertion site


Left flankDirect repeatRight flankDR Length
TTTATTCGCTATCGATGAATAGGGAATAAG10
GATTGGGTTTATAGTTGGTTAGTGTTTAGG10

DNA sequence

CATGTACAGGATCTTTAGCGCCAACCAATTGAAATAGATATAAAAAAGGCTTCGTTTGTGATCTAATTTTATCGCCAAACAAAATCAACACGCGCGAAGC
CTATGACCATTATTAATGCTAATCACTATCTGGAGCAACTCCTGGCTCCCGCCGCACTCGAGCGCATTGCTCGGCTCTGCAAGTTTTGTCTGCGTCAGCG
CGCCATTACCCCTGCCATGCTCGTACCTGCACTGTTGCGGGCCATGGGGGGCGACCAGGTCAATGACATTGCCAGCTTACACCGTCATTTCAATGCCTTG
CAGCTGACGAAAGAGCATCAGGTTTCTTACAAACCGTTTCACAATCAGCTGCGTAAAGAGAGCTTTGCCCTGTTTATGAAAGCGCTGGTCGAACGGGCCA
TCGCCTTGCGTATCGACCATCAACTCAAAGCGGCCTCCCTCGGCGCGTTCAAGCAGGTGTTATTGCACGATGGCACCTCCTTCGCGGTGCACCGGCGTCT
GGCGGCTGACTTTCCCGGCCGGTTCAAGACCATCAGTCCGGCGGCTATCGAGTGCCATATGACCATGTCTCTGTCGGAGCAGAGCCCACTGTGCATGAGT
GTGTCTGCCGATACCGCAGGGGAGCGGCAATTTCTCCCTGAGGCTCACAAGCTGGCCAACTGCCTGCTGCTGGCTGACGCGGGTTACATTGACAGGGCCT
GGTTTGAGCAGGTGAACGACGCTGGCGGATTTTATCTGGTGCGAGGAACGCAAAGCCTGAACCCCAAGATAATCCAGGCCTGGCGCGGTGATGGACGGGA
AGTGCCAAAACTGGCGGGACTGTCACTGAAAGAGGCAGGACGGCGGCGCTGTCGGGCCGAGGTGCTGGATATGGTGGTGAAGTCCGGCAAGGTCGAGTAC
CGCTTGATCCGACGCTGGTTTGCCGAGGAAAAGCGGTTCTGCTTGTGGATGACCAACCTGCCGCGAGCGGCATGGTCAGCAGAGCAGGTGATGAGCCTGT
ATCGCTGCCGTTGGCAGGTGGAGTTGTTATTTAAAGAGTGGAAATCGCACAACCGGCTCAAGGGGTTTGTGACGGGGGAGAAAGCCATCGCCGAGGGATT
GGTGTGGGCGAGTTTGCTGTCGCTGGTGATGAAAAGGCGGGTCGCGCAGAGTGTGATGACAGGGAAGCTATCGATGTTGAAAGCGTCAAAGAACAGTACG
ACATGGTGGTTGCCGCTATTGGAAGCAGTGGCCCATAGAGCGCTGACAGAAATAAGGGAAAGACTGGAATGGGCAGCTGATTATTTGGCCAAGAACGCTT
GCCGAACCAAGCAGAGAAAATCGATACAGAACAGGACCTTGGAAGGAGTTTTAAAGGGACTCAACGCTTAAGGTCCTGTACATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1269 bp422 aa1031371+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTIINANHYLEQLLAPAALERIARLCKFCLRQRAITPAMLVPALLRAMGGDQVNDIASLHRHFNALQLTKEHQVSYKPFHNQLRKESFALFMKALVERAI
ALRIDHQLKAASLGAFKQVLLHDGTSFAVHRRLAADFPGRFKTISPAAIECHMTMSLSEQSPLCMSVSADTAGERQFLPEAHKLANCLLLADAGYIDRAW
FEQVNDAGGFYLVRGTQSLNPKIIQAWRGDGREVPKLAGLSLKEAGRRRCRAEVLDMVVKSGKVEYRLIRRWFAEEKRFCLWMTNLPRAAWSAEQVMSLY
RCRWQVELLFKEWKSHNRLKGFVTGEKAIAEGLVWASLLSLVMKRRVAQSVMTGKLSMLKASKNSTTWWLPLLEAVAHRALTEIRERLEWAADYLAKNAC
RTKQRKSIQNRTLEGVLKGLNA

 

Blast result :
Comments
ISAeme3 is 97% aa similar to ISApu1.
IS_pep_1 is B224_0743.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694