ISAeme2

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1212 bp

Ends


IR Length : 19/22

IRL : CAGGGCGGGATCGCACTTTGTTAATGAAATTTGAACAGGCTGATCGTCTG
IRR : CAGGGCGGGAGCATACTTTGTTGGTTATTTAACCAAAGAATAGTTGTGCC

Insertion site


Left flankDirect repeatRight flankDR Length
TCTGAATTCCCGGTTCACATGGCGGTCTGA10
TTCATGCATAGCATTGAGAAGTTCACTAGT10

DNA sequence

CAGGGCGGGATCGCACTTTGTTAATGAAATTTGAACAGGCTGATCGTCTGTGATCTAATCACGTCTCCATGCAGGAGGCGCGCTCTATGACCTTAATTGA
ACACCTTACCCTTGTTGAAGAAACCCGCTCTGATATCAATCGTAAACATGATCTTGTTGATGTCATGTTTCTTGTCATCAGCGCCATCATTAGTGGCGCC
GAGGGCTGGCGGGACATAGAGACCTATGGCAATTCAAAGGCCGATTGGCTCCGTCAGTATCGCCCCTTTGCCAATGGCATCCCGCGCCGTCACACCATTG
CTCGCATTCTGCGCTGCATTGTCATCGATACCCTGCTTGAAGCATTGCTGTGCTGGGTCAATGAACAGCGCACTCATCAGGGAAAACCCATCATTGCCTT
TGATGGCAAGGTGTTACGCGGCTCCTTTCGGGGAAATGCCAAGGATGCTCTGCAGTTGGTCACGGCCTACGATACCGAAAATGGGCTGGTGCTAAGCCAG
AAAGCAACCCCGAACAAGAAGGGGGAAATCGAAACCGTCAAAGAGATGCTGGACATTCTTGAACTCAAGGGGGCCGTTGTCACGCTTGATGCCCTGCATT
GTCAGCGAGAAACATTGCAGAAAATCAGCGAGAAGAAGGCTCATGTGGTGGTACAGGTAAAGAAGAATCAGCCAAAGTTGTACCAAGCCGTTCAGTCACA
ATTCCAGGCACTGTTTGACGCTCAGAAAGAGAAGATTGTCGTCGAGCACAAAGAGAGTGGGCATGGTCGTCAAGAAGAACGCTATGTGTTTCAATTAAAA
GCAAAATTACCGCCAGAGTTAGCAGAGAAATGGCCGACAATCCGCAGCATCATTGCCGTTGAGCGCCACCGCAGCGCCAACGGAAAAGGAACGGTAGATA
CCTCCTACTATGTCAGTTCGTTATCCCCCAAGCACAAGCTGCTCGGGCACTATATTCGTCAGCATTGGCGGATAGAGAACAGCCAGCATTACATTCTTGA
TATGGTGTTTAACGAGGATGCATCAAGGATAGCGATGGAGGATGCAGTCGAGAATATGGCGCTATTTAGACGGTTTGTTCTGAACATAGTAAAACAGAGT
GATTGCGGCGCACCGAGCCAGAGAAACAAGCTAAAAAAAGCAAGCTGGGATGACAATTATCGGGCACAACTATTCTTTGGTTAAATAACCAACAAAGTAT
GCTCCCGCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1098 bp365 aa871184+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MTLIEHLTLVEETRSDINRKHDLVDVMFLVISAIISGAEGWRDIETYGNSKADWLRQYRPFANGIPRRHTIARILRCIVIDTLLEALLCWVNEQRTHQGK
PIIAFDGKVLRGSFRGNAKDALQLVTAYDTENGLVLSQKATPNKKGEIETVKEMLDILELKGAVVTLDALHCQRETLQKISEKKAHVVVQVKKNQPKLYQ
AVQSQFQALFDAQKEKIVVEHKESGHGRQEERYVFQLKAKLPPELAEKWPTIRSIIAVERHRSANGKGTVDTSYYVSSLSPKHKLLGHYIRQHWRIENSQ
HYILDMVFNEDASRIAMEDAVENMALFRRFVLNIVKQSDCGAPSQRNKLKKASWDDNYRAQLFFG

 

Blast result :
Comments
ISAeme2 is 97% aa similar to ISAs1.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694