ISfinder Mirror Site

ISHel1

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AOLK01000011	ND	Haloferax elongans	Haloferax elongans ATCC BAA-1513

DNA section

IS Length : 1563 bp

Ends

Left end : CTGTAATTAGGCGCTAAGTCCCCTTCCTCAAGGAGAAACGTAGGGAGCGAAGCGACCGAGTAGCGCAGTAGGGAGGGGATACAGCGCCGCACGGTTCTCA II struct. : Yes
Right end : GGATACGCCTGTATCTGCAAAGCGCGTCGTGGAAACAGGAAGCCCCACCCTCAAGGAGCGAACCGCGTCAGCGGTGAGCGAGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TGAATTCGAAGA	TTAA	TCGGACGCAGCG	tcac

DNA sequence

CTGTAATTAGGCGCTAAGTCCCCTTCCTCAAGGAGAAACGTAGGGAGCGAAGCGACCGAGTAGCGCAGTAGGGAGGGGATACAGCGCCGCACGGTTCTCA
AACACGTTCACCGCCCGGCCCACAGGCCACCCCCACCTTTATGTCTATCCGAAGTCTATACATCGCTTATGGATCGATTTGAAATCGAAGGCGAAGAAGT
CCTCGACGGAACCGCAAAACCGTCGGGGAATAGCGCCCACGTCATCGTTCCCAAACGCTGGCGCGGAGCCGACGTGAAAGTCGTCCGCGTCTCCGAACCC
GATTCAGACGAGTAAGACATGCAGTACAACTACAAGTATCGACTCAATCCGACAGAAGACGTCGCTGAGACGCTTCTACACCACGTCGATACTTGTAGGC
AACTGTACAACCACGTCCTCCACCTACTCAACGAGGCAGACACCATCCCCGCCCGCTACGAAGTGCAGGGGCGACTTCCCGACCTCAAATCATGGTGGAC
TGATCTCGGAGGCGTTCACTCGAAAGTTCTCCAGATGGTCGTCAAGCGCGTCTATGACAACCTCTCCACGCTCAAAGCGCAGAAGGAGAACGGACGTACC
GTGGGGATGCTCAAGTGGAAACCGCCTCGGGAGTATCGGTCGCTCACCTACAACCAGACCGGCTTCAAACTCAAGAATACGAGTGGTCGGCCTACGTTGT
GGTTGAGCAAAATCGGAGAAATACCGATTCAATTCCACCGAGACATCCCCGAGAACGCGACCATCAAACAGGTCACGGTCAAGCAGGAACCAACGGGTGG
GTGGTACGCCACGTTCGGCATCGACGTGGACGAAGCCACGACGGAGAAACCTGAGAAACCAGAGCAAGTTGTGGGTATCGACGTGGGGATTCTCAAGTAC
GCCCACGACACCGATGGGTACGCTATCGAGGGTCCCGACTTCAGCGAGGAACGTGAGCGACTCGAACGCGTACAACGCGACCTCTCGCGGAAGGAACGCG
GCTCTGCGAATTGGAAAAAACAACGTCGGGTTGTTGCCGAATGCCACGCCGACCTGAAGCGGAAACGGCGAGACTTCTTGCACAAACTTTCGAACTACTA
CGCCACTGAGTACGACTTGGTGGCCGTTGAGGATTTGGACGCGAAGGGACTGGTCGAACTGCCGGGCAACTCGCGCAACCGGGCAGGAGCGGCGTGGGGG
ACGTTTCTGCGGATGCTCAAATACAAGTGCGAGCGCGAAGGAACGCACTTCGTCGCAGTTGACCCGAAAGATACGACGAAAGAGTGTGCGTCCTGCGGTG
TCAAGACAGACAAACCGCTGTGGGTTCGTGAACACTCGTGTCCTTCGTGTGGGTTCGAGGCGGATAGGGACGCGAATGCGGCGTGGAACATTCTTTCTCG
CGGTCTCGAAGATGTAGGTGTGGGATACTCCGAATCAACGCCTGTGGAGACTGCGCTCCCTGTGGATACGCCTGTATCTGCAAAGCGCGTCGTGGAAACA
GGAAGCCCCACCCTCAAGGAGCGAACCGCGTCAGCGGTGAGCGAGTAGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
147 bp	48 aa	169	315	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDRFEIEGEEVLDGTAKPSGNSAHVIVPKRWRGADVKVVRVSEPDSDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1230 bp	409 aa	319	1548	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MQYNYKYRLNPTEDVAETLLHHVDTCRQLYNHVLHLLNEADTIPARYEVQGRLPDLKSWWTDLGGVHSKVLQMVVKRVYDNLSTLKAQKENGRTVGMLKW
KPPREYRSLTYNQTGFKLKNTSGRPTLWLSKIGEIPIQFHRDIPENATIKQVTVKQEPTGGWYATFGIDVDEATTEKPEKPEQVVGIDVGILKYAHDTDG
YAIEGPDFSEERERLERVQRDLSRKERGSANWKKQRRVVAECHADLKRKRRDFLHKLSNYYATEYDLVAVEDLDAKGLVELPGNSRNRAGAAWGTFLRML
KYKCEREGTHFVAVDPKDTTKECASCGVKTDKPLWVREHSCPSCGFEADRDANAAWNILSRGLEDVGVGYSESTPVETALPVDTPVSAKRVVETGSPTLK
ERTASAVSE

Blast result :

Comments

ISHel1 is 91% aa similar to ISNamo25. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.