ISfinder Mirror Site

ISHam3

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AOLW01000014	ND	Haloarcula amylolytica	Haloarcula amylolytica JCM 13557

DNA section

IS Length : 1551 bp

Ends

Left end : CTGAATTGAGGCGCTAAGGCCCCTTCCTCAAGGAGCGACCGAAGGGAGCGAGTAGGGAGGGGATACAGCGCCGCACAGTTCTCATACACGATTCGGTGGC II struct. : Yes
Right end : TACCAATTCGGTGTCTGCAAAGCGCGTCGTTGAAGCAGGAAGCCCTACCCTCAAGGAACGAGCCGCGTCAGCGGCGAGTGAGTAGGGTAGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TCATCCCGCTGT	TTAC	TGTGTCGTCAGC	tcac

DNA sequence

CTGAATTGAGGCGCTAAGGCCCCTTCCTCAAGGAGCGACCGAAGGGAGCGAGTAGGGAGGGGATACAGCGCCGCACAGTTCTCATACACGATTCGGTGGC
AGGCCCACAGGCCCACCGCCACGTTTATGTTGAAACGAAGTATAAACATAGTTGATGGACAGGCACGAGATAGAAGGCCACGAGGTCATCACCGGGACGG
TGAAAGCGACTGGTAACGGCGCTCACGTCCTCGTTCCGAAGGACTGGCGTGGCGCGGATGTGAAAATCGTCCGGACAAGCGAAGCCACTGACGAATAGCA
CAACAGATGAACTACAACTACAGGTATCGGCTTCGTCCGTCCGACGCTCTCGAAGACCAGTTAGCGTGGACTGTAGATACCTGTAGACAGGTCTACAACC
ACTTCCTCCACCGACTCAATCAACACGACGATACCTCGGCATACTCCGAGCAAAAACGGCTTCCCGACCTCAAGAAGTGGTGGACCGACCTGAAAGACGT
TCACTCGAAGGTTCTCCAGAAAGTCGTTCAACGGCTGTACGACAACCTCTCCACGCTCCGTGGCCGCAAGGAGAACGGCCACCATGTCGGGACGCTCAAC
TGGAAGGCACCCGGCGAGTACCGCAGTTTCACGTACAGTCAATCCGGCTTCAAGCTCAAAAACACGAGCGGTCGGACGCGACTGTGGCTCTCGAAACTCG
GAGAAATCCCGCTCACCTTCCACCGTGACCTGCCCGACAACGCTGATATTAAGACCGTCACAATCAAGCAGGAACCGACCGGGAAATGGTACGCCGTCCT
CGGCATCGAAACGCCTGACTCTCCACCTGCGAAGCCCGAGAATCCCGGGAAGTGCGTCGGAATCGACGTGGGGATTCTCAAGTACGCTCACGACACCGAC
GGAACTGCCGTCGAATCGCCAGACCTCGCAACCGAACGCGAGCGGCTGGAACACGCACAGCGGGAACTATCGCGGAAGGAACACGGGTCAGAAAATTGGG
AGAAACAGCGGAAGATTGTAGCCGAACGCCACGCCGACCTCAAGCGAAAGCGTCGTGATTTCCTTCACAAACTGTCGAACTACTACGCCACCGAGTACGA
CTTGGTGGCCGTAGAGGACTTGGACGCGAAGGGCTTGGTCGAACTGCCGGGCAACTCGCGCAAGCGGGCAGGCGCGGCGTGGGGAACGTTCCTGCGGATG
CTCAAATACAAGTGCGAACGCGAAGGCACGCATTTCGTCGCCGTGAACCCGAGCGGGACGACCAAGGAGTGCGCGTCGTGCGGTGTTTCGACGGACAAGC
CGTTGTGGGTCCGTGAACACTCCTGTCCAGCCTGCGGATTCGAGGCGGACAGGGACGCGAACGCGGCATGGAACATCCTTTCTCGCGGCCTCGAAGATGT
AGGAGTGGGATACTCCGAATCAACGCCTGTGGAGACTGCGCTCCCTGTGGATACCAATTCGGTGTCTGCAAAGCGCGTCGTTGAAGCAGGAAGCCCTACC
CTCAAGGAACGAGCCGCGTCAGCGGCGAGTGAGTAGGGTAGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
144 bp	47 aa	155	298	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDRHEIEGHEVITGTVKATGNGAHVLVPKDWRGADVKIVRTSEATDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1230 bp	409 aa	307	1536	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYNYRYRLRPSDALEDQLAWTVDTCRQVYNHFLHRLNQHDDTSAYSEQKRLPDLKKWWTDLKDVHSKVLQKVVQRLYDNLSTLRGRKENGHHVGTLNWK
APGEYRSFTYSQSGFKLKNTSGRTRLWLSKLGEIPLTFHRDLPDNADIKTVTIKQEPTGKWYAVLGIETPDSPPAKPENPGKCVGIDVGILKYAHDTDGT
AVESPDLATERERLEHAQRELSRKEHGSENWEKQRKIVAERHADLKRKRRDFLHKLSNYYATEYDLVAVEDLDAKGLVELPGNSRKRAGAAWGTFLRMLK
YKCEREGTHFVAVNPSGTTKECASCGVSTDKPLWVREHSCPACGFEADRDANAAWNILSRGLEDVGVGYSESTPVETALPVDTNSVSAKRVVEAGSPTLK
ERAASAASE

Blast result :

Comments

ISHam3 is 91% (TnpB) aa similar to ISHko4. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission.
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.