ISfinder Mirror Site

ISHalo1

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AOLI01000019	ND	Haloferax larsenii	Haloferax larsenii JCM 13917

DNA section

IS Length : 1671 bp

Ends

Left end : TGGATTTAGGCGCTATGTCCCCTTCCTCAAGGAGCAACGCAGGGAGCGGAGCGACCGAGTAGCGCAGTAGGGAGGGGATACAGCGTTCACGAGAGCAAAG II struct. : Yes
Right end : GGACACCGTTGTGTCTGCAAAGCGCGTCGTGGAAACAGGAAGCCCCACCCTCAAGGAGCGAACCGCGTCAGCGGTGAGCGAGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TCCCGAGTTAAT	TTAAC	TGGCTGCTTACT	tcac

DNA sequence

TGGATTTAGGCGCTATGTCCCCTTCCTCAAGGAGCAACGCAGGGAGCGGAGCGACCGAGTAGCGCAGTAGGGAGGGGATACAGCGTTCACGAGAGCAAAG
CTCTCGTGCGCAAACGAGACGCGAGGCGTCTCGTCAACGCCGCACGATTCTCAAACACGTTCACCACCCGGCCCACAGGCCCACTGCCACCTTTATATCT
ATACATAGTATATACATCGGTGATGGATAGGTTTGAAATCGAAGGTGAAGAAGTCCTTGACGGAACGGCAAAGCCGTCGGGGAACAGTGCCCACGTCATC
GTTCCCAAACGCTGGCGTGGAGCCGACGTGAAAGTCGTTCGTGTCTCCGAACCCGACTCAGACGAATAGATGCACTACAACTACAAGTATCGACTCAACC
CGACAGAAGCCCTCACAGAGACGCTTCTGCACCACGTCGATACTTGTAGGCAACTGTACAATCACGTCCTCTACAAACTCAACGAGGAAGACGGCATTCC
TGCTCGCTACGAGGTTCAGGGGAAACTTCCCGACCTCAAAACGTGGTGGGACGACCTCGGAGACGTTCACTCGAAAGTTCTCCAAATGGTCGTCAAGCGC
GTCTACGACAATCTCTCCACGCTCAAAGCGCAGAAGGAGAATGGGCACGCCGTGGGAATGCTCAAGTGGAAACCGCCCCGGGAGTATCGGTCGCTCACCT
ACAACCAGTCCGGCTTCAAACTCAAGAATACGAGTGGTCGGCCTGTCTTGTGGTTGAGTAAAATCGGTGAGATTTCCATTCACCTTCACCGAGACATTCC
CGAGAACGCGACCATCAAACAGGTCACGGTCAAACAAGAACCCACGGGCGAGTGGTACGCCACGTTCGGTATCGACGTGGACGAAGCCACGCCGGAAAAA
CCTGAGAAGCCAGAGCAAGTCGTTGGTATCGACGTGGGGATTCTCAAGTACGCCCACGATACTGACGGGTACGCCATCGAAAGCCCCGACTTCAGCGAGG
AACGTGGGCGACTCGAACGCGCACAACGCGACCTTTCGCGGAAGGAACACGGCTCTGCGAATTGGGAAAAACAACGTCGGGTTGTTGCCGAATACCACGC
CGACCTGAAGCGCAAGCGGCGAGACTTCTTGCACAAGTTGTCGAACTACTACGCGACCGAGTACGACTTGGTGGCCGTTGAGGATTTGGACGCGAAGGGA
TTGGTCGAACTCCCGGGCAACTCTCGTAATCGAGCGGGAGCGGCGTGGGGGACGTTCCTGCGGATGCTCGAATACAAGTGCGAACGCGAAGGCACGCACT
TCGTCGCGGTTGACCCGAAAGATACGACGAAAGAGTGCGCGGCCTGTGGAGCCAAAACGGATAAGCCGCTGTGGGTGCGCGAACATTCCTGTCCCTCGTG
TGGGTTCGAGGCGGACAGGGACGCGAACTCGGCGTGGAACATTCTTTCTCGCGGTCTCGAAGATGTAGGAGTGGGATACTCCGAATCAACGTCCTCAGAA
TCGCTCTGCGATTCTGATGTGCGAACGAGAGATTTGCTCTCGTTAACGCCTGTGGAGACTGCGCTCCCTGTGGACACCGTTGTGTCTGCAAAGCGCGTCG
TGGAAACAGGAAGCCCCACCCTCAAGGAGCGAACCGCGTCAGCGGTGAGCGAGTAGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
147 bp	48 aa	223	369	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDRFEIEGEEVLDGTAKPSGNSAHVIVPKRWRGADVKVVRVSEPDSDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1287 bp	428 aa	370	1656	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MHYNYKYRLNPTEALTETLLHHVDTCRQLYNHVLYKLNEEDGIPARYEVQGKLPDLKTWWDDLGDVHSKVLQMVVKRVYDNLSTLKAQKENGHAVGMLKW
KPPREYRSLTYNQSGFKLKNTSGRPVLWLSKIGEISIHLHRDIPENATIKQVTVKQEPTGEWYATFGIDVDEATPEKPEKPEQVVGIDVGILKYAHDTDG
YAIESPDFSEERGRLERAQRDLSRKEHGSANWEKQRRVVAEYHADLKRKRRDFLHKLSNYYATEYDLVAVEDLDAKGLVELPGNSRNRAGAAWGTFLRML
EYKCEREGTHFVAVDPKDTTKECAACGAKTDKPLWVREHSCPSCGFEADRDANSAWNILSRGLEDVGVGYSESTSSESLCDSDVRTRDLLSLTPVETALP
VDTVVSAKRVVETGSPTLKERTASAVSE

Blast result :

Comments

ISHalo1 is 89% (TnpB) aa similar to ISNamo25. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T.(2012) Direct submission GenBank.