ISHko4

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
EMA70404 ND Halorubrum kocurii
Halorubrum kocurii JCM 14978
DNA section
IS Length : 1517 bp

Ends


Left end : CTGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAACGAGCGAAGCGAGTGAGTAGGGAGGGGATACAGCGCCGCACGGTTCTCAAACACGTTCGACGCTC II struct. : Yes
Right end : CTGTGTCCTCGGTTGTGGATGCAAAGCGCGTCGTGGAAACAGGAAGCCCCACCCTCAAGCGCGAGGGCGTCAGCCCGAGCGGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
AACAACATTAATTTACCTGCGACATTTGtcac

DNA sequence

CTGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAACGAGCGAAGCGAGTGAGTAGGGAGGGGATACAGCGCCGCACGGTTCTCAAACACGTTCGACGCTC
GGCCCACAGGCCCACGCAATCGAAACAACTATGCGCACAGATACGCATAGTGCGTATGTAGTGAGGCGGAAGAACATCACCATCCGCGAAGACCAAGCCG
AGTGGGTCGAGGAGAACCACCTTAACCTCTCGTCGTTCGTTCGCGGACAACTTGACGAACTCATCGAAGAACGCTCGTGAACTACAACTACAGGTATCGA
CTCCGACCGTCCGACGCTCTCGAAGACCAGTTAGTGTGGACTGTCGATACCTGTAGACAGGTCTACAACCACTTTCTCCACCGACTCAACCGCACCGACG
ACACCTCGGCATACTCCGAGCAGAAGCTCCTGCCGAGTCTCAAGAAGTGGTGGAGCGGCCTGAAAGACGTTCACTCGAAGGTACTACAGAAGGTCGTGCA
ACGGCTGTACGACAACCTCTCGACACTACGAGAGCGCAAAGAGAACGGCTACCGCGTCGGGACGCTCAAGTGGAAGGCACCGGACGAGTACCGCAGTTTC
ACCTACAGTCAATCCGGCTTCAAGCTCAAGAACACGGGCGGTCGGACGCGACTGTGGCTCTCAAAACTCGGAGAAATCCCGCTCACCTTCCACCGCGACC
TCCCCGACGACGCCGACATTAAGACCGTCACGGTCAAGCAAGAACCTACCGGGAAGTGGTACGCCATTCTCGGGATCGAGACGCCCGACGACCCGCCAGA
GAAGCCGGAGAATCCCGAGAACTGCGTCGGTATCGACGTGGGGATTCTCAAGTACGCCCACGACACCGACGGAACCGCCGTCGAATCCCTCGACCTGTCC
GACGAACGCGAACGTTTGGAACGCGCACAGCGCGACCTCTCGCGGAAACAGCACGGGTCGGACAATTGGGAGAAACAGCGTCAGGTCGTGGCAGAACGCC
ACGCCGAGTTGAAGAACAAGCGCCGCGACTTCCTTCACAAGCTCTCGAACTACTACGCTCGGGAGTACGAGTTCGTGGCCGTCGAGGACTTGGACGCAAA
GGGGTTGGTCGAACTACCGGGCAACTCGCGGAACCGGGCAGGTGCGGCGTGGGGGACGTTCCTGCGGATGCTCAAATACAAGTGCGAACGCGAAGGAACG
CACTTCGTTGCTGTGAATCCGCGCGGAACGACGAAAGAGTGTGCATCCTGCGGCGTTTCGACCGAGAAACCGTTGTGGGTGCGCGAACACTCCTGCCCGG
CGTGTGGGTTCGAGGCGGACAGAGATGCGAACGCGGCGTGGAATATTCTTTCTCGCGGTGTGAAGAAGCGGTTAGGAGCGGGACACTCCGAATCAACGCC
TGTGGAGACTGCGCTCCCTGTGTCCTCGGTTGTGGATGCAAAGCGCGTCGTGGAAACAGGAAGCCCCACCCTCAAGCGCGAGGGCGTCAGCCCGAGCGGT
AGGGTGGGGTAGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
120 bp39 aa161280+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRRKNITIREDQAEWVEENHLNLSSFVRGQLDELIEERS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1236 bp411 aa2771512+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYNYRYRLRPSDALEDQLVWTVDTCRQVYNHFLHRLNRTDDTSAYSEQKLLPSLKKWWSGLKDVHSKVLQKVVQRLYDNLSTLRERKENGYRVGTLKWK
APDEYRSFTYSQSGFKLKNTGGRTRLWLSKLGEIPLTFHRDLPDDADIKTVTVKQEPTGKWYAILGIETPDDPPEKPENPENCVGIDVGILKYAHDTDGT
AVESLDLSDERERLERAQRDLSRKQHGSDNWEKQRQVVAERHAELKNKRRDFLHKLSNYYAREYEFVAVEDLDAKGLVELPGNSRNRAGAAWGTFLRMLK
YKCEREGTHFVAVNPRGTTKECASCGVSTEKPLWVREHSCPACGFEADRDANAAWNILSRGVKKRLGAGHSESTPVETALPVSSVVDAKRVVETGSPTLK
REGVSPSGRVG

 

Blast result :
Comments
ISHko4 is 93% (TnpB) aa similar to ISHla22. The first ORF is a passenger gene annotated as hypothetical protein.
References
1] Friedhelm Pfeiffer (2015) Direct submission.
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.