ISHko3

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AOJH01000009 ND Halorubrum kocurii
Halorubrum kocurii JCM 14978
DNA section
IS Length : 1862 bp

Ends


Left end : CTGGATATAGGCGCTAAGCCCCCACCCTCAACGGAGCGATCGAAGGGAGCGGAGTAGGGTGGGGATACAGCGCCGTCATCATCTGTTCATACAGCGCCGT II struct. : Yes
Right end : CACCGACTCGGTGTCTGCAAAGCGCGTCGTGGAAGCAGGAAGCCCTACCCTCAAGGAGCGAACGGCGTCAGCCGTGAGCGAGTAGGGTAGGGTAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GATCTCGATTCGTTAGCGATGGACGGTGTCAC

DNA sequence

CTGGATATAGGCGCTAAGCCCCCACCCTCAACGGAGCGATCGAAGGGAGCGGAGTAGGGTGGGGATACAGCGCCGTCATCATCTGTTCATACAGCGCCGT
CATCATCTGTTCATACAGCAATACGGTTGCAGGCCCACAGACCCACGTAGTCACAACGTTTTTTCACTGGGGTACCGTACAATAGTACGAACCCAATGGA
GTACGACCTCGATTCGGGAGCGCATTCGACGTACTCCCTGCACTACCACCTGATACTCACTACGAAGTATCGGCGCGGAGTGCTAACCGAGGAGCGAACC
CAATTCATTCGCGGGGTTATCAGCGGGTTCACGGACAACTACGGCGTCGAACTGACGAACCTCGACGGAGAGGACGACCACGTTCACATCCTGTTCCGAG
CGAAACCCACCACAAACCTCGTGAAGTTCATTAATACAGTCAAGGGCGCGACTGCTCGCCGTATCCGAAACGAGTACGAGGATGAGCTGAAGACCGAACT
GTGGGGCGACTCGTTCTGGAACGACTCGTACTGCCTCATCTCGACGGGGCAGGTGTCGCTGGATGTGCTGAAACAGTACGTCGAAGACCAGCGGGAGAGC
TGATGTACTACGCCTACAAGTACCGTCTCAAGCCGTCCGACGCCCACCGAAAGGAGTTGGACCGCCACCGCGATATTTGTAGGCAATTGTACAACCACAC
GCTCTATCGGCTCAACGAGTACCAAGACGAACACGGCGAACTACCTTCCATGACCACGCTTCGGTCGGAACTCCCCGACCTCAAGAACTGGTGGGACGGC
CTCTCCGACGTGTACTCGAAGGTTCTCCAAACCGTCGTGGAACGGCTGTTCGACAACCTCAAAAGCCTCTCCAAACTCAAAGAGAACGGCTACGGCGTCG
GTCAACTCAAGTGGAAGCCGCCACGGGAGTTCCGCAGTTTCACGTACAACCAGTCTGGCTTCAAGCTCGACAAAAAGGGCGGTCAGACTGTGCTGTCACT
CTCGAAACTCGGAGACATACCGATTCGGCTCCACCGCGCCATCCCCGACGACGCGAAGCTCAAACAGGTCACGATCAAGAAGGAACCGACGGGCGAGTGG
TTCGCCACGTTCGGCGTCCAAATGGACCGTGAACCGCCCGAACCGCCTGAGAATCCCGAGCGGTGCGTCGGCATTGACGTGGGGATTCTCAAGTACGCCC
ACGACACCGACGGGACCGCCGTCGGGTCGCCCGACCTCTCCGCCGAACGGGAGCGTCTGGAACGCGAACAGCGGTCCCTCTCACGGAAGGAATACGGGTC
GAACAACTACGAGAAGCAACGACGGCGGGTCGCGGAGTGTCACGCCGATCTCCGACGAAAGCGCCGCGACTTCCTGCACAAGCTCTCGAACTACTACGCT
CGGGAGTACGACCTCGTGGCGGTCGAAGACCTGAACGTGAAAGGGATACTCGAATCACCGTCGAACAGCCGCAACACGGCGGCGGCGGCGTGGCGAACGT
TCCTCACATTACTCGAATACAAGTGCGAGCGTGAAGGAACGCACTTCGTCGCCGTGAATCCGAGAGGGACGACCAAGGAGTGCGCGTCGTGCGGCGTTTC
GACCGACAAACCGTTGTGGGTCCGTGAACACACCTGTCCCGCCTGCGGGTTTAAGTCGGACAGGGACGCAAACGCGGCGTGGAACATCCTTTCTCGCGGC
CTCGAAGATGTAGGAGTGGGACACTCCGAATCAACGTCTGTGGAGACTGCGCTCCCTGTGGACACCGACTCGGTGTCTGCAAAGCGCGTCGTGGAAGCAG
GAAGCCCTACCCTCAAGGAGCGAACGGCGTCAGCCGTGAGCGAGTAGGGTAGGGTAGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
408 bp135 aa196603+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MEYDLDSGAHSTYSLHYHLILTTKYRRGVLTEERTQFIRGVISGFTDNYGVELTNLDGEDDHVHILFRAKPTTNLVKFINTVKGATARRIRNEYEDELKT
ELWGDSFWNDSYCLISTGQVSLDVLKQYVEDQRES

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1245 bp414 aa6031847+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MYYAYKYRLKPSDAHRKELDRHRDICRQLYNHTLYRLNEYQDEHGELPSMTTLRSELPDLKNWWDGLSDVYSKVLQTVVERLFDNLKSLSKLKENGYGVG
QLKWKPPREFRSFTYNQSGFKLDKKGGQTVLSLSKLGDIPIRLHRAIPDDAKLKQVTIKKEPTGEWFATFGVQMDREPPEPPENPERCVGIDVGILKYAH
DTDGTAVGSPDLSAERERLEREQRSLSRKEYGSNNYEKQRRRVAECHADLRRKRRDFLHKLSNYYAREYDLVAVEDLNVKGILESPSNSRNTAAAAWRTF
LTLLEYKCEREGTHFVAVNPRGTTKECASCGVSTDKPLWVREHTCPACGFKSDRDANAAWNILSRGLEDVGVGHSESTSVETALPVDTDSVSAKRVVEAG
SPTLKERTASAVSE

 

Blast result :
Comments
ISHko3 is 97% (TnpA : the transposase) aa similar to ISHmu6 and 96% (TnpB) aa similar to ISHma12.
References
1] Friedhelm Pfeiffer (2015) Direct submission.
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.