ISfinder Mirror Site

ISNpe31

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003372	ND	Natrinema pellirubrum	Natrinema pellirubrum DSM 15624

DNA section

IS Length : 1667 bp

Ends

Left end : TCTTTGAGCAGCGAGAGAATCCCGCCGTTCACGGCGGGCGTGAATCGCGTCACTCGACTACGCAAACCACGAGCGACAGCACTCTGAATACCCTCTGTCA II struct. : Yes
Right end : GACCGGTGATGCGAATACGTCTTGAAACCTCAATCCAGCACTTTGCTGGTATTCCACCTGCGGCGGAAGCCCCGGCGTTCACGCCGGGCGAGGATGTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GCGGCTCTCGA	TCTA	TGAACGGCTTAG	tcac

DNA sequence

TCTTTGAGCAGCGAGAGAATCCCGCCGTTCACGGCGGGCGTGAATCGCGTCACTCGACTACGCAAACCACGAGCGACAGCACTCTGAATACCCTCTGTCA
ACACAAATCTTTAAGGTACAAAGAAACGTACTGAAAAACGGATGCGAGTTGACATCGCCATTGAACGTGGAAGCGACCTCCACGAACGAGTGAAAGAATA
CTCTCGTGAGAACGGCCTGCGAATGGACTTCGCATACGCCGAACTCATCGAACAAGGACTCGACGCTGAAGAATGAGTGAGGAGTGCAAGACGCTCGAAG
CGTATCTTGCCCCACCCACTCAGCACAAGCAGCGCAAGCTCCACGACGAACACGACCGATACGAGAAACTACTCCGTCGTAGCTTCAACAACGAGTGCGA
TACGATGACCGCCGTCAACGAGGTCGTCACAGGTGAAGACCTCAACTGGCACTCGAAAAACGCTCTCAAACAATACGTGCCCAACTTGCTTGACGAGGAC
ACGTATGATGCGAAGCAACTCGCAGACACTCATCCAATCCGCTACGCCAACACCGCATCCGTCTTCGACCACGATGAAGACCGCCACCACGAAATTTGCT
GGGAAGTCCCGCTACCCGGTCGTGGAACGAACTTCTGGATACCACTCCAGTTGAACCCAGAGCAAGAAGACTGGTGGCACTCTCTGATTAACGACGAAGA
CGAGAGTGTATGGGCGGGCGAACTCCGCTTACAACGCGACGGTGATAGATGGGTGTTGCACGTCACCGCGAACTACGAAGTCGAACCAGACCACTCGTAT
TCGTGTGAGAGCGACGATATGACACCCGTCGGGTTTGACGTGGGTGAATCCAAACTCTTGGTGGGCTGTGCCCTCCGAGACAACACACCCGTTGACCCAT
TATTCGTGGATGGTGGTCGCGTCCGACACCTCCGACAGAAACAATCCAGTGCCGAAGACCGCTTGAAACCCCGATACGCCTCAAAGCTCTTGGACGACCT
CGTGTGGGGTCGGTGGCAAGACGCCATCGAAGACGAAATCGAGAAAGCCTCCGCACGAGCCGTCGAATACGCTGCACAGTTCGAGAACCCGGTTATCGTT
TTCGAATACCTCGACGGAATCACCGACGAGGACATTGGGAAGTACTGGAATCGACGCCTCGGCAAGTGGCTGTTCTCCCAACTACAGTCACGCATCGAGG
ACAAGGCAGCGGAACACGGCATTCCTGTGGAATACGTGTACCCGCATCACACGTCGAAGACGTGCCATGCGTGCCAGCATATCGGGTATCGACCGCATCA
GGGCACGTTCAAGTGTACGAACGAGGAGTGTTGGGTGTCGGAGTATCAAGCGGATTTGAACGCAGCAGCGAATATAGCCAATCGGCTAAATCCGTGGGGA
GAGAGCCTACCAGTGAAATGGGTGGGCGATGACTCGCCTCGGAGCGGGGGCCAGTGGCAGGCCCACCAAGACACGTTACCGATCGAGGGACTCCCGTCCG
AAAGGCGGGCTTCCGATGACAAGGTTTCGTCTAGCTCACCAACGGTGGGAGCGGGGGCGAAGCCAGAGACCGGTGATGCGAATACGTCTTGAAACCTCAA
TCCAGCACTTTGCTGGTATTCCACCTGCGGCGGAAGCCCCGGCGTTCACGCCGGGCGAGGATGTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
135 bp	44 aa	142	276	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRVDIAIERGSDLHERVKEYSRENGLRMDFAYAELIEQGLDAEE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1320 bp	439 aa	273	1592	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MSEECKTLEAYLAPPTQHKQRKLHDEHDRYEKLLRRSFNNECDTMTAVNEVVTGEDLNWHSKNALKQYVPNLLDEDTYDAKQLADTHPIRYANTASVFDH
DEDRHHEICWEVPLPGRGTNFWIPLQLNPEQEDWWHSLINDEDESVWAGELRLQRDGDRWVLHVTANYEVEPDHSYSCESDDMTPVGFDVGESKLLVGCA
LRDNTPVDPLFVDGGRVRHLRQKQSSAEDRLKPRYASKLLDDLVWGRWQDAIEDEIEKASARAVEYAAQFENPVIVFEYLDGITDEDIGKYWNRRLGKWL
FSQLQSRIEDKAAEHGIPVEYVYPHHTSKTCHACQHIGYRPHQGTFKCTNEECWVSEYQADLNAAANIANRLNPWGESLPVKWVGDDSPRSGGQWQAHQD
TLPIEGLPSERRASDDKVSSSSPTVGAGAKPETGDANTS

Blast result :

Comments

ISNpe31 is 93% (TnpB) aa similar to ISHwa29. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Pfeiffer F. (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission to GenBanK.