ISfinder Mirror Site

ISHme3

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001868	ND	Haloferax mediterranei	Haloferax mediterranei ATCC 33500

DNA section

IS Length : 1530 bp

Ends

Left end : AGGCGGTTCAAGGCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAGCCGACAGTGGCCGACCAGCCGCCGGGTTGTTTCTCCCTTCCCACAGGCCCACGG II struct. : Yes
Right end : CGCCTGTGCAGACTGCGCTCCCTACGGGACCCCTTTCGGTTCCTGCAAAGCGCGTCGTAGAAGCAGGAAGCCCCGAGGCTTGACCTCGGGGTGGGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TCTGGATTTGAT	TCTAC	TGCTGCTGTCTC	tcac

DNA sequence

AGGCGGTTCAAGGCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAGCCGACAGTGGCCGACCAGCCGCCGGGTTGTTTCTCCCTTCCCACAGGCCCACGG
TATCGAAACACTCACTTCGAGCGAATCAGAATGAGTGTGTATGGGTCAGACAGAGATGGAATGTTACCCGACTGTCCGTGACCGGGGACAGGTCACGATT
CCCGAAGATGTGCGCGAACCGCTCGGTATCGAGCCGGGCGACCGAATCAAACTCACTGTCGAACGACTGGACTAACTCAGATGGACGTAACGCTTCGCTG
TCGTGCGTATCCCGACGAGGAAACCGCCAGCGAAGCGTGGCGTCACATCGAAATCCATCGACAGATACGAAACCACGCCATCCGCGACTACTACTCACAC
GACTACGGAAACCGGCCAACCGCCTACGACCAGCACCGCAAACTCACCGGCTGGAAACAGCGGTGGCCGCTCTTTTCGGAAGTTTCGGCACACGCCGCCC
AACAGACTGTTTCGGAGATACACAAGAACGTCGAGACACAGAAGGGTCGGCGGGAAAACGGCCACAAGACGGGGCGGTTGAAGTGGCAGGGAAGCGGCGA
CTGTCGGTCAGTCGCGTATCAGACCGAAGGCTTCGACGTGAATCACACCACGGGCCAAGACGAGTACGCCACTGTTCGACTCTCGAAAATCGGCAACATC
CCGATTCGAGCGCACCGAGAGCTTCCCGCGACCGAGAACATCAAGCGCGTCGTGTTAAAGAAGGAGCGAACTGGCGACTGGTTTGTCTGTCTCGTCACGG
AACGCCCGGACGAGGACCTTCCCGAAAAACCCGACCCCAGTACGCTCGACCCTGCCGACTGCGTAGGGGTAGACCTCGGGATTCTCTCGTATATCCACAC
CAGCGACGATACGGCGGTGGATATGCTCGACCTGTCCGACGAGTACGACCGCTACGGGCGCGAACAGCGGAAACTCGACCGCAAACAGCACGGCTCGAAC
AACTGGGAGAAACAACGGCAGAAGGTCGCTGCGGCGAAGCGGCAAATCAAACGGAAAGTGCTGGACTATCAGCACAAACTCACGACGTGGCTGGTCCGAA
AGTACGATTTGGTGGCCGTCGAGGATTTGGACGTAAAGCCGATGCTGGAAACCAGCCAGAATGCCAAGAACAAGCAGGACGCCGCATGGTCGCGGTTTCT
GGACCTCTTGGAGTACAAAGCCGACCTGCATGGCACTCACGTCGTTCGAGTCAAACCAGCAGGAACGACCAAAGAGTGTTCGGAGTGCGGTGTGGAAACG
TCAAAGCCACTCTGGATTCGAGAACACTCTTGTCCGGCGTGCGGTCACACCGAGGACAGGGACCTGAACGCGGCGAAGAACATCCTTTCTCGCGGTCGCT
CACAGATAGGGGCGGGACGCTCCGAATCAACGCCTGTGCAGACTGCGCTCCCTACGGGACCCCTTTCGGTTCCTGCAAAGCGCGTCGTAGAAGCAGGAAG
CCCCGAGGCTTGACCTCGGGGTGGGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
135 bp	44 aa	141	275	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGQTEMECYPTVRDRGQVTIPEDVREPLGIEPGDRIKLTVERLD

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1233 bp	410 aa	281	1513	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MDVTLRCRAYPDEETASEAWRHIEIHRQIRNHAIRDYYSHDYGNRPTAYDQHRKLTGWKQRWPLFSEVSAHAAQQTVSEIHKNVETQKGRRENGHKTGRL
KWQGSGDCRSVAYQTEGFDVNHTTGQDEYATVRLSKIGNIPIRAHRELPATENIKRVVLKKERTGDWFVCLVTERPDEDLPEKPDPSTLDPADCVGVDLG
ILSYIHTSDDTAVDMLDLSDEYDRYGREQRKLDRKQHGSNNWEKQRQKVAAAKRQIKRKVLDYQHKLTTWLVRKYDLVAVEDLDVKPMLETSQNAKNKQD
AAWSRFLDLLEYKADLHGTHVVRVKPAGTTKECSECGVETSKPLWIREHSCPACGHTEDRDLNAAKNILSRGRSQIGAGRSESTPVQTALPTGPLSVPAK
RVVEAGSPEA

Blast result :

Comments

ISHme3 is 81% (TnpB) aa similar to ISHwa19. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission.
2] Han, J., Zhang, F., Hou, J., Liu, X., Li, M., Liu, H., Cai, L., Zhang, B., Chen, Y., Zhou, J., Hu, S., Xiang, H. (2012) J. Bacteriol. 194, 4463-4464