ISRtr5
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DQ499058 | ND | Rhizobium tropici | Rhizobium tropici CFN299 Rhizobium leucaenae USDA 9039 |
DNA section
IS Length : 2577 bp
Ends
IR Length : 18/24
IRL : GTAAGCGCACAATTCGCGGCACCTGTTCATTGAAAATCGAATGAAGGAGT
IRR : GTAAGCGCGCATTTCCCTGCGCGTTGACGCGGGCGCTATGAGGGTGATCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAAAGCAGTC | GAGGAAGT | CGTTCCATTC | 8 |
DNA sequence
GTAAGCGCACAATTCGCGGCACCTGTTCATTGAAAATCGAATGAAGGAGTCTGTGTCCACAACGGAGACTGTGGACATAATGGCATTGGATATCTTGACT
GGATCGGAGCCCCTTCGGGCTGTGAAGGTTCTTTCCAACGGGAAGCGACGTTTCGATCCCAACGAGAAAGACCGTCTGATCGAGGCTGCAATGAAGCCCG
GCGTATCAGTCGCCCGCCTGGCCCTGGCGCGCGGCGTCAACGCCAATCAGTTACGCAATTGGGTGAAGCTTCGCCGAAATCGGCAGGCCCAAGACACTTT
GACGGTCGTTGCAGGGCAGGAAACGTCGGCCTTTGTTCCGGTGGTTGCAGCGTCGCCGACTGCGCGGCAGCCCGACATGCCGGCGCGGTCATCATCGGCA
GGAATGCGGCTGATGGCGTCTTTGCCGAATGGTGTGCGGCTTGAACTTGAGAATGTGGATGAGCGTGCCCTTTGCGCGATGATCGAAGTTCTGGGGCGGT
GCAATGTTCCGGCTGGCTGACGATCTTCGCGTCTATCTTCACCGGGATCCGATCGACTTTCGTGCGGGGATCAACAGCCTGGCGATCCTGGTCGAGCAGT
CGATGGGTCTGAGCCCGTTCGAGCGTGCTGTGTTCGTTTTTTGCAATCGCCGGCGAACCCGGATGAAGCTCCTGTTCTTCGAGCGGTCAGGGTTTGTGCT
CGTCTTGAAAGCCTTGTCCGAAGACCGGTTTCGCTGGCCCCGCCGCGAGGCGCCGGTCGTGATGCTCGATACCGAACAGTTGCGCTGGCTGCTTGACGGC
ATCGATCTCGACGCGATGGTGCGCCATCCTGTCCGGCAGTATGAATTTGTCGGCTGAAGGCAGTTGACGGCGGGGGGCGGCTCAGATTCAAAGCTGTGAT
GACTCCAGCCGGCACCCCGACCGTTGCGGAACTGATGGCGCTTCTGGCGGCGAATGCTGCCGAGAACGCTGCGCTGCGGGCTGAAAGAGACGCCCTTGAG
CAGCGTGTCTTCAAGCTCGAGGAAGAGCTGGCGCTTGCACAACTGCATCGTTTTGCGCCGCGCAGCGAAAAGCATATGGATCGCGTCTTCAACGAAGCCG
AGAACGCCGCCCTTGAGGCCGATGCTGATGAGGGCTTGGCCGATTGTGGCGAGCCGGACGTCGCAGAGCTTCCCGACACGGGATTGCCAGAGGTAGAAAA
GCCCGAAGGCAGGAAACGCGGACGCAAACCTCTTCCGGCAAACTTGCCACGCCAACGCGTTGAATATGACCTTGCCGACGATCAGAAGACCTGTCCGTGC
TGCCGCCATCCGTTGCATCGCATGGGTGAACTCGTCACCGAGCAGCTGCATATCGAAGTGAAGGCCACGGTGCTGCAAAATGCCCGGGCCAAGTACGCTT
GCCGCAACTGCGAGCGCACCGATATCAGCACGCCCGTTATCATCGCGCCGATGCCCGCACAGCCCTTGCCGGGCAGTATTGCCACGGCCTCGACGCTGGC
TTTTGCTCTTGTTCATAAATATGTCGACGGCACACCGCTCTATCGCCTGGCACAGGCCTTCGAGCGCGCCGGCGTTCCTGTCAGCCGTGGCGCTCTTGGC
CATTGGGTGATCGGTTCCAGCGAGAAGCACCTCGTTCGCATCTACGATGCCCTGAAACAGCGGCTCCTATCGCAGGACGTCATTCACGGTGACGAAACCA
CGGTGCAGGTGCTGAAGGAAAAGGACAGGAAAGCAACCGACGAGTCCTACATCTGGGCCTATCGCAGCGGGCAGGACAGCGTCGAGCCGGTTGTGCTACT
CGAATATCAGCCCGGTCGTGGGCAGGTGCATCCGCAGACTTTCCTGGGTGACTATCGCGGCATCTTGATGAGCGACGGCTATCAGGCCTGGCGCACCCTC
AAGGGGGCAACGCATGTCGGATGCATGGCTCACGCCAGGCGACGCTTCGTCAACGCCTTCAAGGCCGGGAAGAAACAAAGCGGCCCGCCCACACAGGCAT
TGAAGTTCTTCGACCAGCTCTATCGGATTGAAAGGCAGGTGCGGGACGAAAAGCCGGATGACGGCGAAACGCAAGCCGATTGCCTCCGGCGCCTGCGCCA
GAAGCACAGCGTTCCAGTCCTTGACGCCCTGAAGGAGTGGCTCGACAGCATGGCCCCCAAGGTCGTGCCCGATACCAAGCTCGGCGATGCGATCTCCTAT
ACGCGAAACCAGTGGCAATATCTGACGCGCTACACCGAAGACGGCAGGATGCCGATCGACAACAATTTATTGGAGCGCGACATTAGAGTTTTTGCAACCG
GAAGAAAATCCTGGCTCTTCTGTGACACTGTCGACGGGGCCAAGGCAAGTGCCGTAATCTACAGCCTCATGCTGACCTGTCGCGCCTGTGGCGTCGAGCC
ATTCGCCTGGTTGCGACACGTCTTCACCGAATTGCCGCAGCGTCCAGACGGCGCCGACATCAACGACCTCCTACCATTCAACTTCGCAAAAACCGCACCC
GCCTGATCAAGGCCCATCGGCAAAAGACGATCACCCTCATAGCGCCCGCGTCAACGCGCAGGGAAATGCGCGCTTAC
GGATCGGAGCCCCTTCGGGCTGTGAAGGTTCTTTCCAACGGGAAGCGACGTTTCGATCCCAACGAGAAAGACCGTCTGATCGAGGCTGCAATGAAGCCCG
GCGTATCAGTCGCCCGCCTGGCCCTGGCGCGCGGCGTCAACGCCAATCAGTTACGCAATTGGGTGAAGCTTCGCCGAAATCGGCAGGCCCAAGACACTTT
GACGGTCGTTGCAGGGCAGGAAACGTCGGCCTTTGTTCCGGTGGTTGCAGCGTCGCCGACTGCGCGGCAGCCCGACATGCCGGCGCGGTCATCATCGGCA
GGAATGCGGCTGATGGCGTCTTTGCCGAATGGTGTGCGGCTTGAACTTGAGAATGTGGATGAGCGTGCCCTTTGCGCGATGATCGAAGTTCTGGGGCGGT
GCAATGTTCCGGCTGGCTGACGATCTTCGCGTCTATCTTCACCGGGATCCGATCGACTTTCGTGCGGGGATCAACAGCCTGGCGATCCTGGTCGAGCAGT
CGATGGGTCTGAGCCCGTTCGAGCGTGCTGTGTTCGTTTTTTGCAATCGCCGGCGAACCCGGATGAAGCTCCTGTTCTTCGAGCGGTCAGGGTTTGTGCT
CGTCTTGAAAGCCTTGTCCGAAGACCGGTTTCGCTGGCCCCGCCGCGAGGCGCCGGTCGTGATGCTCGATACCGAACAGTTGCGCTGGCTGCTTGACGGC
ATCGATCTCGACGCGATGGTGCGCCATCCTGTCCGGCAGTATGAATTTGTCGGCTGAAGGCAGTTGACGGCGGGGGGCGGCTCAGATTCAAAGCTGTGAT
GACTCCAGCCGGCACCCCGACCGTTGCGGAACTGATGGCGCTTCTGGCGGCGAATGCTGCCGAGAACGCTGCGCTGCGGGCTGAAAGAGACGCCCTTGAG
CAGCGTGTCTTCAAGCTCGAGGAAGAGCTGGCGCTTGCACAACTGCATCGTTTTGCGCCGCGCAGCGAAAAGCATATGGATCGCGTCTTCAACGAAGCCG
AGAACGCCGCCCTTGAGGCCGATGCTGATGAGGGCTTGGCCGATTGTGGCGAGCCGGACGTCGCAGAGCTTCCCGACACGGGATTGCCAGAGGTAGAAAA
GCCCGAAGGCAGGAAACGCGGACGCAAACCTCTTCCGGCAAACTTGCCACGCCAACGCGTTGAATATGACCTTGCCGACGATCAGAAGACCTGTCCGTGC
TGCCGCCATCCGTTGCATCGCATGGGTGAACTCGTCACCGAGCAGCTGCATATCGAAGTGAAGGCCACGGTGCTGCAAAATGCCCGGGCCAAGTACGCTT
GCCGCAACTGCGAGCGCACCGATATCAGCACGCCCGTTATCATCGCGCCGATGCCCGCACAGCCCTTGCCGGGCAGTATTGCCACGGCCTCGACGCTGGC
TTTTGCTCTTGTTCATAAATATGTCGACGGCACACCGCTCTATCGCCTGGCACAGGCCTTCGAGCGCGCCGGCGTTCCTGTCAGCCGTGGCGCTCTTGGC
CATTGGGTGATCGGTTCCAGCGAGAAGCACCTCGTTCGCATCTACGATGCCCTGAAACAGCGGCTCCTATCGCAGGACGTCATTCACGGTGACGAAACCA
CGGTGCAGGTGCTGAAGGAAAAGGACAGGAAAGCAACCGACGAGTCCTACATCTGGGCCTATCGCAGCGGGCAGGACAGCGTCGAGCCGGTTGTGCTACT
CGAATATCAGCCCGGTCGTGGGCAGGTGCATCCGCAGACTTTCCTGGGTGACTATCGCGGCATCTTGATGAGCGACGGCTATCAGGCCTGGCGCACCCTC
AAGGGGGCAACGCATGTCGGATGCATGGCTCACGCCAGGCGACGCTTCGTCAACGCCTTCAAGGCCGGGAAGAAACAAAGCGGCCCGCCCACACAGGCAT
TGAAGTTCTTCGACCAGCTCTATCGGATTGAAAGGCAGGTGCGGGACGAAAAGCCGGATGACGGCGAAACGCAAGCCGATTGCCTCCGGCGCCTGCGCCA
GAAGCACAGCGTTCCAGTCCTTGACGCCCTGAAGGAGTGGCTCGACAGCATGGCCCCCAAGGTCGTGCCCGATACCAAGCTCGGCGATGCGATCTCCTAT
ACGCGAAACCAGTGGCAATATCTGACGCGCTACACCGAAGACGGCAGGATGCCGATCGACAACAATTTATTGGAGCGCGACATTAGAGTTTTTGCAACCG
GAAGAAAATCCTGGCTCTTCTGTGACACTGTCGACGGGGCCAAGGCAAGTGCCGTAATCTACAGCCTCATGCTGACCTGTCGCGCCTGTGGCGTCGAGCC
ATTCGCCTGGTTGCGACACGTCTTCACCGAATTGCCGCAGCGTCCAGACGGCGCCGACATCAACGACCTCCTACCATTCAACTTCGCAAAAACCGCACCC
GCCTGATCAAGGCCCATCGGCAAAAGACGATCACCCTCATAGCGCCCGCGTCAACGCGCAGGGAAATGCGCGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 441 bp | 146 aa | 80 | 520 | + | No |
AG : IS66 TnpA
ORF sequence :
MALDILTGSEPLRAVKVLSNGKRRFDPNEKDRLIEAAMKPGVSVARLALARGVNANQLRNWVKLRRNRQAQDTLTVVAGQETSAFVPVVAASPTARQPDM
PARSSSAGMRLMASLPNGVRLELENVDERALCAMIEVLGRCNVPAG
PARSSSAGMRLMASLPNGVRLELENVDERALCAMIEVLGRCNVPAG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 354 bp | 117 aa | 504 | 857 | + | No |
AG : IS66 TnpB
ORF sequence :
MFRLADDLRVYLHRDPIDFRAGINSLAILVEQSMGLSPFERAVFVFCNRRRTRMKLLFFERSGFVLVLKALSEDRFRWPRREAPVVMLDTEQLRWLLDGI
DLDAMVRHPVRQYEFVG
DLDAMVRHPVRQYEFVG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1644 bp | 535 aa | 899 | 2542 | + | No |
Chemistry : DDE
ORF sequence :
MTPAGTPTVAELMALLAANAAENAALRAERDALEQRVFKLEEELALAQLHRFAPRSEKHMDRVFNEAENAALEADADEGLADCGEPDVAELPDTGLPEVE
KPEGRKRGRKPLPANLPRQRVEYDLADDQKTCPCCRHPLHRMGELVTEQLHIEVKATVLQNARAKYACRNCERTDISTPVIIAPMPAQPLPGSIATASTL
AFALVHKYVDGTPLYRLAQAFERAGVPVSRGALGHWVIGSSEKHLVRIYDALKQRLLSQDVIHGDETTVQVLKEKDRKATDESYIWAYRSGQDSVEPVVL
LEYQPGRGQVHPQTFLGDYRGILMSDGYQAWRTLKGATHVGCMAHARRRFVNAFKAGKKQSGPPTQALKFFDQLYRIERQVRDEKPDDGETQADCLRRLR
QKHSVPVLDALKEWLDSMAPKVVPDTKLGDAISYTRNQWQYLTRYTEDGRMPIDNNLLERDIRVFATGRKSWLFCDTVDGAKASAVIYSLMLTCRACGVE
PFAWLRHVFTELPQRPDGADINDLLPFNFAKTAPA
KPEGRKRGRKPLPANLPRQRVEYDLADDQKTCPCCRHPLHRMGELVTEQLHIEVKATVLQNARAKYACRNCERTDISTPVIIAPMPAQPLPGSIATASTL
AFALVHKYVDGTPLYRLAQAFERAGVPVSRGALGHWVIGSSEKHLVRIYDALKQRLLSQDVIHGDETTVQVLKEKDRKATDESYIWAYRSGQDSVEPVVL
LEYQPGRGQVHPQTFLGDYRGILMSDGYQAWRTLKGATHVGCMAHARRRFVNAFKAGKKQSGPPTQALKFFDQLYRIERQVRDEKPDDGETQADCLRRLR
QKHSVPVLDALKEWLDSMAPKVVPDTKLGDAISYTRNQWQYLTRYTEDGRMPIDNNLLERDIRVFATGRKSWLFCDTVDGAKASAVIYSLMLTCRACGVE
PFAWLRHVFTELPQRPDGADINDLLPFNFAKTAPA
Blast result :
Comments
ISRtr5 is 78% (ORFA), 95% (ORFB) and 94% (ORFC : the transposase) aa similar to IS692.
The original sequence of ISRtr5 is truncated on the left end and the transposase is non functional, we replace this sequence by a complete one found in Rhizobium leucaenae USDA 9039 (AUFB01000066) which present 99% of DNA identity.
The original sequence of ISRtr5 is truncated on the left end and the transposase is non functional, we replace this sequence by a complete one found in Rhizobium leucaenae USDA 9039 (AUFB01000066) which present 99% of DNA identity.
References
1] Hernandez-Lucas,I., Xianwu,G., Gaytan,M.A., Ramirez-Trujillo,A., Perez-Rueda,E., Flores,M., Broughton,W., Martinez-Romero,E. and Mavingui,P. (2006) FEMS Microbiol. Lett. 261 (1), 25-31.
2] Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2013) Direct submission GenBank.
2] Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2013) Direct submission GenBank.