ISWpi17
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Wolbachia pipientis | Wolbachia pipientis Wolbachia endosymbiont of Armadillidium vulgare strain C (wVulC) |
DNA section
IS Length : 1287 bp
Ends
IR Length : 20/26
IRL : TATAATGCACCCCATAAACTAGACCAAAAAAAAATGGTTAATCCGAGTAG
IRR : TATAGTGCACCCATTTGTCTAGACCATTTTTTTCAAAGTGATCCGATTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTGCACCTCA | CTA | TTCACTGGGT | 3 |
| CTGATCTGGG | CTTT | CCTGAACATT | 4 |
| ACGATAAGGC | AA | CTGCAGGAAC | 2 |
| TACTTGATAT | CAGTTTAGTA | 0 | |
| AGCATCTAAT | GTTCCTTATT | 0 | |
| CTCCATTTTC | CCTGGATCAA | 0 |
DNA sequence
TATAATGCACCCCATAAACTAGACCAAAAAAAAATGGTTAATCCGAGTAGGAAGGTAAAGGGGTAAAAGTATGGCAACAAAAAAATATGAACCAGAGTTA
AAAGCAAAGATAGCTTTGGAAGCAATAAAAAATCAAAAAAGCACATCTGAGATATGTAGTGAATATAAAATACCATCAACAAATCTATATGATTGGCGTG
ATAGAGTATTGGCAAGGTTAAAAGACCTATTTGTTGAAGAAAGTGAAAGTGCGAGAAAACAAAGAATCTTAGCGCAAGAAATAGAAAGTTTACATAAAGT
AATAGGAGAATTGACAGTGGAAAATAGTTATTTGAAAAAAAAATTACTGAAATAAGCAAAAAAGATAGAGTAAGGTTTATAGAAAAAGATTCTGATCTGT
CAATTAGGAAACAGGCTGATTTATTGGGGATTTGCAGATCTAGCCTATATTATAGGCCTATAATTAATAACGAAAGTGAAGTAGCAAATTTGATTCAATT
GGCTTCTGATTGCCGTTATGGATATCGTAAAATTACTGCTGAAATCATAGCGAGTGGAGTAGTAGTCAATCACAAAAAAATCTTAAGAATTATGAAAAAA
ATGAAGATTAGTGGGCTGTATTGTAGAAAAAGATGTAATACAAGTATTAAAGAAAAAAAGCATAAAATATATCCTTATTTACTCAAAGATTTGATTATTT
GCAGAGTTAATCAGGTATGGGCTACTGATATAACATATATTATGGTAGAAGGTAAGTTTGTCTATTTTGTGGCAATAATGGACTTGTATAGTCGCTATAT
TATTGCTCATTCATTATCACCATATCTCGATGCTGGATTTTGCCTTTATACTCTCAAAGAAGCTCTAAAACAAGGTAAACCTGAGATTTTTAATAGTGAT
CAAGGGATGCAGTTTACTAGCTACAACTTTATTATGGAATTAGAGCGTGCTAATATTAAAATCAGTATGGACCATAAAGGACGTTGCTTCGACAATATAT
TTGTTGAGCGCTTATGGAGAACTTTAAAGCAAGAAGCTATATATTATTATAGACCAAATAGTATCAGAGATTTGAATCTTATAATAAATGATTTTGTTGC
TTGGTATAACTATAGAAGGCGACATCAGACTCTACATTATAAAGTTCCTGCTGATCTTTATTATCATAAACAGTAAATGAATATGTTGATAAATACTTTA
AATGTGTGTCGACGTATTTATCAACATATTCATTTTAAAAATCGGATCACTTTGAAAAAAATGGTCTAGACAAATGGGTGCACTATA
AAAGCAAAGATAGCTTTGGAAGCAATAAAAAATCAAAAAAGCACATCTGAGATATGTAGTGAATATAAAATACCATCAACAAATCTATATGATTGGCGTG
ATAGAGTATTGGCAAGGTTAAAAGACCTATTTGTTGAAGAAAGTGAAAGTGCGAGAAAACAAAGAATCTTAGCGCAAGAAATAGAAAGTTTACATAAAGT
AATAGGAGAATTGACAGTGGAAAATAGTTATTTGAAAAAAAAATTACTGAAATAAGCAAAAAAGATAGAGTAAGGTTTATAGAAAAAGATTCTGATCTGT
CAATTAGGAAACAGGCTGATTTATTGGGGATTTGCAGATCTAGCCTATATTATAGGCCTATAATTAATAACGAAAGTGAAGTAGCAAATTTGATTCAATT
GGCTTCTGATTGCCGTTATGGATATCGTAAAATTACTGCTGAAATCATAGCGAGTGGAGTAGTAGTCAATCACAAAAAAATCTTAAGAATTATGAAAAAA
ATGAAGATTAGTGGGCTGTATTGTAGAAAAAGATGTAATACAAGTATTAAAGAAAAAAAGCATAAAATATATCCTTATTTACTCAAAGATTTGATTATTT
GCAGAGTTAATCAGGTATGGGCTACTGATATAACATATATTATGGTAGAAGGTAAGTTTGTCTATTTTGTGGCAATAATGGACTTGTATAGTCGCTATAT
TATTGCTCATTCATTATCACCATATCTCGATGCTGGATTTTGCCTTTATACTCTCAAAGAAGCTCTAAAACAAGGTAAACCTGAGATTTTTAATAGTGAT
CAAGGGATGCAGTTTACTAGCTACAACTTTATTATGGAATTAGAGCGTGCTAATATTAAAATCAGTATGGACCATAAAGGACGTTGCTTCGACAATATAT
TTGTTGAGCGCTTATGGAGAACTTTAAAGCAAGAAGCTATATATTATTATAGACCAAATAGTATCAGAGATTTGAATCTTATAATAAATGATTTTGTTGC
TTGGTATAACTATAGAAGGCGACATCAGACTCTACATTATAAAGTTCCTGCTGATCTTTATTATCATAAACAGTAAATGAATATGTTGATAAATACTTTA
AATGTGTGTCGACGTATTTATCAACATATTCATTTTAAAAATCGGATCACTTTGAAAAAAATGGTCTAGACAAATGGGTGCACTATA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 71 | 355 | + | No |
Description : First part of the transposase
ORF sequence :
MATKKYEPELKAKIALEAIKNQKSTSEICSEYKIPSTNLYDWRDRVLARLKDLFVEESESARKQRILAQEIESLHKVIGELTVENSYLKKKLLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 849 bp | 282 aa | 328 | 1176 | + | No |
Description : Second part of the transposase
ORF sequence :
LFEKKITEISKKDRVRFIEKDSDLSIRKQADLLGICRSSLYYRPIINNESEVANLIQLASDCRYGYRKITAEIIASGVVVNHKKILRIMKKMKISGLYCR
KRCNTSIKEKKHKIYPYLLKDLIICRVNQVWATDITYIMVEGKFVYFVAIMDLYSRYIIAHSLSPYLDAGFCLYTLKEALKQGKPEIFNSDQGMQFTSYN
FIMELERANIKISMDHKGRCFDNIFVERLWRTLKQEAIYYYRPNSIRDLNLIINDFVAWYNYRRRHQTLHYKVPADLYYHKQ
KRCNTSIKEKKHKIYPYLLKDLIICRVNQVWATDITYIMVEGKFVYFVAIMDLYSRYIIAHSLSPYLDAGFCLYTLKEALKQGKPEIFNSDQGMQFTSYN
FIMELERANIKISMDHKGRCFDNIFVERLWRTLKQEAIYYYRPNSIRDLNLIINDFVAWYNYRRRHQTLHYKVPADLYYHKQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1106 bp | 368 aa | 71 | 1176 | + | Yes |
Chemistry : DDE
ORF sequence :
MATKKYEPELKAKIALEAIKNQKSTSEICSEYKIPSTNLYDWRDRVLARLKDLFVEESESARKQRILAQEIESLHKVIGELTVENSYLKKKITEISKKDR
VRFIEKDSDLSIRKQADLLGICRSSLYYRPIINNESEVANLIQLASDCRYGYRKITAEIIASGVVVNHKKILRIMKKMKISGLYCRKRCNTSIKEKKHKI
YPYLLKDLIICRVNQVWATDITYIMVEGKFVYFVAIMDLYSRYIIAHSLSPYLDAGFCLYTLKEALKQGKPEIFNSDQGMQFTSYNFIMELERANIKISM
DHKGRCFDNIFVERLWRTLKQEAIYYYRPNSIRDLNLIINDFVAWYNYRRRHQTLHYKVPADLYYHKQ
VRFIEKDSDLSIRKQADLLGICRSSLYYRPIINNESEVANLIQLASDCRYGYRKITAEIIASGVVVNHKKILRIMKKMKISGLYCRKRCNTSIKEKKHKI
YPYLLKDLIICRVNQVWATDITYIMVEGKFVYFVAIMDLYSRYIIAHSLSPYLDAGFCLYTLKEALKQGKPEIFNSDQGMQFTSYNFIMELERANIKISM
DHKGRCFDNIFVERLWRTLKQEAIYYYRPNSIRDLNLIINDFVAWYNYRRRHQTLHYKVPADLYYHKQ
Blast result :
Comments
ISWpi17 is 61% aa similar to ISCysp23.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift. This IS has atypic IRs: it begins with 'TA' instead of 'TG'.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift. This IS has atypic IRs: it begins with 'TA' instead of 'TG'.
References
1] Cerveau N. (2015) Direct submission