ISfinder Mirror Site

ISCsa3

Family IS110
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009437	ND	Caldicellulosiruptor saccharolyticus	Caldicellulosiruptor saccharolyticus DSM 8903 Caldicellulosiruptor lactoaceticus Anaerocellum thermophilum Caldicellulosiruptor krisjanssonii Caldicellulosiruptor bescii

DNA section

IS Length : 1526 bp

Ends

Left end : CCTCCATGCTTAGGGAAGCACAACCATTGATGAAAATAGACTTAAGAAAAGGTTGCAAATAATAAAATTTTTGTTAAACTAAAGTTAACAGGGCAAAGGT
Right end : AGCATTGGTTAGTTTATTATTGCCTTTTTCTGGTTGAATGATCGAATATATTCTATTGTTAACGTTCAAAATTATTTTTGAATGCAATCTATTTTCGTGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAATCCACAT	TAAC	ATAGTTTTTA	4

DNA sequence

CCTCCATGCTTAGGGAAGCACAACCATTGATGAAAATAGACTTAAGAAAAGGTTGCAAATAATAAAATTTTTGTTAAACTAAAGTTAACAGGGCAAAGGT
TGATTTTATTGTTGATGGATATAATACCTCGAACATTAACCTAACCTGATTACCTTTGAGCACAGCCAATTGTCGCCCCATATTATCCTGATATGGGAAC
AGCACGGGTGCTAAACTTCATATTTTCTCAGGTAATCATCCCTGTAATAAATTCAGAATAGGAGAGAATAATATGCAAGATTTACTTGAGATTTGTTGTG
GATTGGATGTTCATAAAGAAACTGTTGTAGCCTGCTTGCTTAAAGGCTCTGTGAATGATGATAAACCCCAAAAAACAATAAGAACATTTTCCACTTTACT
GTCAGGGCTTGAAGAATTAAAAGCATGGCTTGAATCAGAAAACTGCCGTTATGTAGCAATGGAGAGCACAGGAGTTTACTGGCAACCTGTTTATAATATT
TTGGAAAGTGCTTTGGAGGGCACTATGGTAGTTATAGTTGCCAATGCAAGGCATATGAAAAATGTTCCTGGCAAAAAGACTGATATGAAAGATGCAAAAT
GGATTGCAACTCTTTTGAGGGCAGGACTTTTAAATGGGAGTTTTATTCCACCTCAAACAGCAAGAGAATTAAGAGATCTTACAAGATATCGTAAAAGCAT
AATAGAAGAACTGTCATCACAGAAAAACCGCATTGAAAAGTATCTTCAAAGCTGTGGATTTAAACTTTCAAATTTTCTCACAGATATATTTGGTGTATCT
GGAAGAGCAATAATAAACCACATAGCAGAACACGGCAGTATAACCGCAGAAGAAGTTGAAAGTTATGTTAAGACGAGAGCACGAAATAAATTGCAAGAGA
TAAAACTGTCGGTAAATGGCAGGATGAACAGACACCAGCGTGAATTTTTAAGGCTTCTGCTTAAGCATTTAGATGAGACATATGAACATCTTGGTGAAAT
AGAAAAAAATATTAATTCTGAGCTTGAGAAATTTAAAAGGCAGATTGAGCAATTGGATAGCATACCAGGTATAGATAAGACGGCGGCTGCTGCGATAATT
GCTGAAATTGGCATAGATATGAGCAAATTTAAAACTGCAGAACATATTTGTTCATGGGCAGGATTAAGTCCTGGCAATAATGAAAGTGCAGGAAAAAAAA
AGTCCACTCGAGTAACAAAGGGTAATACCTATATCAAGAGGATACTGTGTGAAGTTGCATGGAGTCTAACTCGCGTAAGAGATTCATACCTTGCTACGTG
GTATTGGAAAGTAAAGCAGCGCCGAGGGACAAAGAAAGCAATAGTAGCTCTTGCTAGAAAATTACTGGTTATAATCTATAATATGCTTAAGAATGGCACT
GACTATGATGAAAAAGTTTTTTATGAAGTTCGGAAGAAACAAGAAACGATACGTGTTAAACGACTTATCAATGAGGCTAAAAAGTTGGGTTTAGAAGTTA
TAGTGCCTCAATCAGTTTAAATTTTGATTAAATTTATAAGATTTCCAGCTGATGCCTTTTAGCATTGGTTAGTTTATTATTGCCTTTTTCTGGTTGAATG
ATCGAATATATTCTATTGTTAACGTTCAAAATTATTTTTGAATGCAATCTATTTTCGTGC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1290 bp	415 aa	273	1562	+	No

ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MQDLLEICCGLDVHKETVVACLLKGSVNDDKPQKTIRTFSTLLSGLEELKAWLESENCRYVAMESTGVYWQPVYNILESALEGTMVVIVANARHMKNVPG
KKTDMKDAKWIATLLRAGLLNGSFIPPQTARELRDLTRYRKSIIEELSSQKNRIEKYLQSCGFKLSNFLTDIFGVSGRAIINHIAEHGSITAEEVESYVK
TRARNKLQEIKLSVNGRMNRHQREFLRLLLKHLDETYEHLGEIEKNINSELEKFKRQIEQLDSIPGIDKTAAAAIIAEIGIDMSKFKTAEHICSWAGLSP
GNNESAGKKKSTRVTKGNTYIKRILCEVAWSLTRVRDSYLATWYWKVKQRRGTKKAIVALARKLLVIIYNMLKNGTDYDEKVFYEVRKKQETIRVKRLIN
EAKKLGLEVIVPQSV

Blast result :

Comments

ISCsa3 is 67% aa similar to ISGka2.

References

1] Miriam Land (2010) Direct submission
2] van de Werken,H.J., Verhaart,M.R., VanFossen,A.L., Willquist,K., Lewis,D.L., Nichols,J.D., Goorissen,H.P., Mongodin,E.F., Nelson,K.E., van Niel,E.W., Stams,A.J., Ward,D.E., de Vos,W.M., van der Oost,J., Kelly,R.M. and Kengen,S.W. (2008) Appl. Environ. Microbiol. 74 (21), 6720-6729.
3] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P. (2007) Direct submission GenBank.