ISfinder Mirror Site

ISAsp20

Family IS200/IS605
Group IS200

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_019427	ND	Anabaena sp.	Anabaena sp. 90

DNA section

IS Length : 681 bp

Ends

Left end : CGTTGAAACGGACTTGAGCTTTGAGACAGGGAATTTATTCCCTGGCTGAATTGGCTGGAATGGCTGGATAATTACACATTTATTGTTATTCTTTTAAAAT II struct. : Yes
Right end : AAAGCTAAAATCCCTTAAAACGGATTAAACTCAAAAATAATTAGTCGGTTTTAACCGACTTTAGCTATAAGACGGGGAATTTATTCCCCGGCTAAATCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
ATATAATGTG	AGTC	TGACTAAATA	tcaa

DNA sequence

CGTTGAAACGGACTTGAGCTTTGAGACAGGGAATTTATTCCCTGGCTGAATTGGCTGGAATGGCTGGATAATTACACATTTATTGTTATTCTTTTAAAAT
TCATTATGGCTTTGTGGCGCTTGTATTATCATGTTGTCTGGGCTACGAAAAAACGTGAGCCTTTAATTACCAATGATGTAGAGGAAAAATTTTATGGTTA
TTTAATTGGGAAGGCGGATCATTTAGGGTGTATAATTCATGCTATTGGTGGAATTGAAGACCATATTCATTTTGTGGTTTCTATCCCTCCTAAATTGTCT
ATTGCTGAATTTGTGAAAACTTTAAAAGGTAGTAGTGCTTACCATTATAATCATACTTTTGGTAAAACACAACAATTTGCTTGGCAGGAGGGATATGGTG
TATTTTCTTTAGGAGGTAAACAATTACAAGAAGCTATTGATTATGTAGATAATCAAAAAATACATCATCAAAATAAAACCACAATTCCATTATTAGAACA
AGAAACAGATTTAGATAAACCACCAGAAAATAAAACAAAACCTAAAATCCCAGAAATACCAGGAAATAAATTTCCTGACTAAAAGCTAAAATCCCTTAAA
ACGGATTAAACTCAAAAATAATTAGTCGGTTTTAACCGACTTTAGCTATAAGACGGGGAATTTATTCCCCGGCTAAATCAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
477 bp	158 aa	106	582	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MALWRLYYHVVWATKKREPLITNDVEEKFYGYLIGKADHLGCIIHAIGGIEDHIHFVVSIPPKLSIAEFVKTLKGSSAYHYNHTFGKTQQFAWQEGYGVF
SLGGKQLQEAIDYVDNQKIHHQNKTTIPLLEQETDLDKPPENKTKPKIPEIPGNKFPD

Blast result :

Comments

ISAsp20 is 48% aa similar to ISSpn6.
There is 1 copy in the genome.

References

1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.