ISAsp19

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003285 ND Anabaena sp.
Anabaena sp. 90
DNA section
IS Length : 1652 bp

Ends


Left end : CTTAAACAGCAAGAAGGCTCACACTGTACCTGTACTCAGGTCAGTGTTGAGATGAATTGCGCGTGAGAAATCCGGGTGTAGTCTGACCATATTTGTAGCT II struct. : Yes
Right end : ACCAAAGTCTTAGGGACGTAAAAAATCCATGAAGTCCGAGGCAGTCCCCATGGAATTGGGAAGCCTACACTGTACCGTCAGGTCAGTGTAGGTAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CTGGGGAGCTTTACGATATATACCtcac

DNA sequence

CTTAAACAGCAAGAAGGCTCACACTGTACCTGTACTCAGGTCAGTGTTGAGATGAATTGCGCGTGAGAAATCCGGGTGTAGTCTGACCATATTTGTAGCT
TGCTTCCCGCAGGGTGCGCAGCGAAAACCGGAAAGACTACAACGACGAAACGAGCAAAATATTCGTTTTTTATTCGGTATGTTTTGATTATTTGTGTTAC
CATGAATATACGAACGCGGTAAGGTCGATACCATGTATAGAGCAATTAAAGTTAGGATTTATCCCACTGATGAGCAAGAATCATATTTAGCTCAATGTTT
TGGTAATACTCGATGGTTGTGGAATTATATGCTTAATGCCACTACTGTCGCGGATAAAGAAATAAGCATTGGGCTTTCTAAAGCGGCTATGGATAAGTTG
CTTCCAGGATTAAAAAAGGAATATGAATGGTTAGGACTTGCATACAGTCAAGTGTTGCAACGAGTCACATTCAATCTTTCTAGTGCGTTTGTCAACTTTT
TTGAAGGCCGTGCTAAATTTCCTAACTTTAAATCTAAACATGGTAAACAATCAATTCAATATCCCCAAAATGTGAAACTGATGCCAGAGGATTCTGTGAT
TAAGTTTCCTGGCAATTTAGGGATGATGAAAACCGTGTTCCATAAAGAATTGCCCGATGCAAAATTCACGGCTGTAACAATATCAAGAAATGCAGACGGT
AGGTATTATGCATCTATTCTTTTTAACCAAAATGACAATCCGGTAGTTGCAATTAGAAAAGCTATTGGTGTTGATTTGGGATTGAAAAACTTTGCTATAA
CTTCCGAAGGCTCAAAATATGACCTTCCTAAGAAGCAATTAGCTCTTCTCGAAAAGAATAAAAAACGCAAGCAGAAAAAACTAGCTAAGAAGACCGATAA
AACATCTAATAAACGTCGTAAAGCTAAACGTTTAGTCGCAAAAGTTTCTAGCAAAATAGCTAGAGTCCGTGAAGACTTTCTACACAAGCTATCTCGCAAA
ATAGCATCCGAAAACCAAGTTATTTGTGTAGAAGATTTGGCAGTTAAGAATATGGTTAAAAATCCCAACTTGGCAAAAGCTATTAGTGACTCTTGTTGGG
GAATGTTTCAAACCATGCTCAAATACAAAGCTGAAAGATTTGGTCATACTTATCAAGAAATAGGTCGTTTCTTCCCGTCTTCTCAACTTTGTAGTGAAAC
TCTACTACCAATTCCGGTATTGCAAAAAGGATATGATTCGTTAGGAGTTAGGTTTGTAGATTGCCCTCACTGCCAAAAACAGCATGATAGAGATATCAAT
GCCGCAATAAATATTAGAAATGAAGGTTTGCGACTTTGGGCGTTAGGAACTAGCGCTTCTGCCCTTGGAGGGGATGTAAGACCAAAGTCTTAGGGACGTA
AAAAATCCATGAAGTCCGAGGCAGTCCCCATGGAATTGGGAAGCCTACACTGTACCGTCAGGTCAGTGTAGGTAGTTCAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1161 bp386 aa2331393+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MYRAIKVRIYPTDEQESYLAQCFGNTRWLWNYMLNATTVADKEISIGLSKAAMDKLLPGLKKEYEWLGLAYSQVLQRVTFNLSSAFVNFFEGRAKFPNFK
SKHGKQSIQYPQNVKLMPEDSVIKFPGNLGMMKTVFHKELPDAKFTAVTISRNADGRYYASILFNQNDNPVVAIRKAIGVDLGLKNFAITSEGSKYDLPK
KQLALLEKNKKRKQKKLAKKTDKTSNKRRKAKRLVAKVSSKIARVREDFLHKLSRKIASENQVICVEDLAVKNMVKNPNLAKAISDSCWGMFQTMLKYKA
ERFGHTYQEIGRFFPSSQLCSETLLPIPVLQKGYDSLGVRFVDCPHCQKQHDRDINAAINIRNEGLRLWALGTSASALGGDVRPKS

 

Blast result :
Comments
ISAsp19 is 56% aa similar to ISMma19.
There are 1 complete copy and 1 truncated copy in the genome.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.