ISAsp18
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP003285 | ND | Anabaena sp. | Anabaena sp. 90 |
DNA section
IS Length : 1430 bp
Ends
Left end : CCGCCAGGGAGAGCAATTACAAGAAAGACTGGGAAGTTTGAAAGCCCCTGAGTAAGCATTGCGTAGCAATGCCTTATGTGCTTTTAGACAAGGGATGAAA II struct. : Yes
Right end : TTTAGGAGATGAGTCCTCTACTCAGGCTGGAGAGATCCTGTCTGAGCAAGTCATGTCTTTGATTAAAGAATCCCCGCGTCTTTAGACCGGGGAGTGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GCTCAGGAAA | TTTA | CCCATAGTGC | tcaa |
DNA sequence
CCGCCAGGGAGAGCAATTACAAGAAAGACTGGGAAGTTTGAAAGCCCCTGAGTAAGCATTGCGTAGCAATGCCTTATGTGCTTTTAGACAAGGGATGAAA
AGCGACTCAGGTGGTTTTAACCACCGTCAAGATTTTGCAAGGCGCAATACATTTAGCCATCTTCAACCAACTATAGTAGTATAGGATGGGAGGTGAAAGG
ATGCTAGTTTTTGAGTTTAAAGCTTACGGAAAATCAGTTCAGTTAACAGCAATAGATGATGCAATTCGGACTGCACAGTTCATTCGTAATAGCTGTATTC
GGCTATGGATGGATGTTCAAGGTATAGGTAAAAACGACTTGCAGAAATATTGTGCTGTACTGGCGGCTAATTTCCCTTTTGCTAATGAACTCAATTCAAT
GGCTAGACAATCTTCTGCTGAACGAGCATGGGCTGCTATCACTCGGTTTTATAATAATTGCAAGAAAGGCATTCCTGGTCAGAAGGGATATCCTCAATTC
CAAAAAGATTGTCGTTCTGTTGAATACAAAACATCAGGATGGAAGCTGGCTTTGAATCGTAAATCCATCACTTTCACTGATAAAAAAGGCATTGGGCAGT
TAAAACTTAAAGGGACTCGTGACCTCCATTTCTACCAAATTAACCAAATAAAACGGGTAAGATTGGTAAAACGGGCTGATGGTTATTATTGTCAATTTTG
TATTGATGTCAACCGTCAAGAAAATATAGAACCATCGGGAAATACAATTGGTTTAGATGTAGGTTTGAAAGAATACTACACTGATTCTCATGGGGTGATG
GTTGAGAATCCGAAATTTCTGCGTATTGGCCAAAAGGTTCTTAAACGTTCACAACGCCGTGTTTCCAGAAAAGTCAAAGGTTCAAAAAATCGGGGCAAAG
CTAGACAGGTTTTAGGAAAACGCCACCTCAAAATAAGTAGGAAACGTAAAGACCATGCTATTAAATTAGCACGGTGCGTAGTTCAGTCTAACGACTTGAT
AGCCTATGAAGATTTGAGAATTAAAAACATGGTGAGAAATCATTGTTTAGCTAAGTCAATAAACGACGCATCTTGGTATCAGTTTCGTGTCTGGCTTGAA
TATTTTGGCAAAGTATTTAAGAGAGTCACGGTGGCAGTAAATCCACAATATACAAGCCTTGAATGCTCTAGCTGTGGTGAAGTTGTTAAGAAAACTCTAT
CTACGAGGACACACGTTTGTCAGTGTGGCTGTGTGATGGATAGGGATGAGAATGCAGCTAGAAATATCCTTAGTCGAGGATTGAGTACCGTAGGGCATAC
GGGAACTTTCATGCTAGACATGAGCAACGCTTTAGGAGATGAGTCCTCTACTCAGGCTGGAGAGATCCTGTCTGAGCAAGTCATGTCTTTGATTAAAGAA
TCCCCGCGTCTTTAGACCGGGGAGTGTCAA
AGCGACTCAGGTGGTTTTAACCACCGTCAAGATTTTGCAAGGCGCAATACATTTAGCCATCTTCAACCAACTATAGTAGTATAGGATGGGAGGTGAAAGG
ATGCTAGTTTTTGAGTTTAAAGCTTACGGAAAATCAGTTCAGTTAACAGCAATAGATGATGCAATTCGGACTGCACAGTTCATTCGTAATAGCTGTATTC
GGCTATGGATGGATGTTCAAGGTATAGGTAAAAACGACTTGCAGAAATATTGTGCTGTACTGGCGGCTAATTTCCCTTTTGCTAATGAACTCAATTCAAT
GGCTAGACAATCTTCTGCTGAACGAGCATGGGCTGCTATCACTCGGTTTTATAATAATTGCAAGAAAGGCATTCCTGGTCAGAAGGGATATCCTCAATTC
CAAAAAGATTGTCGTTCTGTTGAATACAAAACATCAGGATGGAAGCTGGCTTTGAATCGTAAATCCATCACTTTCACTGATAAAAAAGGCATTGGGCAGT
TAAAACTTAAAGGGACTCGTGACCTCCATTTCTACCAAATTAACCAAATAAAACGGGTAAGATTGGTAAAACGGGCTGATGGTTATTATTGTCAATTTTG
TATTGATGTCAACCGTCAAGAAAATATAGAACCATCGGGAAATACAATTGGTTTAGATGTAGGTTTGAAAGAATACTACACTGATTCTCATGGGGTGATG
GTTGAGAATCCGAAATTTCTGCGTATTGGCCAAAAGGTTCTTAAACGTTCACAACGCCGTGTTTCCAGAAAAGTCAAAGGTTCAAAAAATCGGGGCAAAG
CTAGACAGGTTTTAGGAAAACGCCACCTCAAAATAAGTAGGAAACGTAAAGACCATGCTATTAAATTAGCACGGTGCGTAGTTCAGTCTAACGACTTGAT
AGCCTATGAAGATTTGAGAATTAAAAACATGGTGAGAAATCATTGTTTAGCTAAGTCAATAAACGACGCATCTTGGTATCAGTTTCGTGTCTGGCTTGAA
TATTTTGGCAAAGTATTTAAGAGAGTCACGGTGGCAGTAAATCCACAATATACAAGCCTTGAATGCTCTAGCTGTGGTGAAGTTGTTAAGAAAACTCTAT
CTACGAGGACACACGTTTGTCAGTGTGGCTGTGTGATGGATAGGGATGAGAATGCAGCTAGAAATATCCTTAGTCGAGGATTGAGTACCGTAGGGCATAC
GGGAACTTTCATGCTAGACATGAGCAACGCTTTAGGAGATGAGTCCTCTACTCAGGCTGGAGAGATCCTGTCTGAGCAAGTCATGTCTTTGATTAAAGAA
TCCCCGCGTCTTTAGACCGGGGAGTGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1215 bp | 404 aa | 201 | 1415 | + | No |
AG : TnpB
ORF sequence :
MLVFEFKAYGKSVQLTAIDDAIRTAQFIRNSCIRLWMDVQGIGKNDLQKYCAVLAANFPFANELNSMARQSSAERAWAAITRFYNNCKKGIPGQKGYPQF
QKDCRSVEYKTSGWKLALNRKSITFTDKKGIGQLKLKGTRDLHFYQINQIKRVRLVKRADGYYCQFCIDVNRQENIEPSGNTIGLDVGLKEYYTDSHGVM
VENPKFLRIGQKVLKRSQRRVSRKVKGSKNRGKARQVLGKRHLKISRKRKDHAIKLARCVVQSNDLIAYEDLRIKNMVRNHCLAKSINDASWYQFRVWLE
YFGKVFKRVTVAVNPQYTSLECSSCGEVVKKTLSTRTHVCQCGCVMDRDENAARNILSRGLSTVGHTGTFMLDMSNALGDESSTQAGEILSEQVMSLIKE
SPRL
QKDCRSVEYKTSGWKLALNRKSITFTDKKGIGQLKLKGTRDLHFYQINQIKRVRLVKRADGYYCQFCIDVNRQENIEPSGNTIGLDVGLKEYYTDSHGVM
VENPKFLRIGQKVLKRSQRRVSRKVKGSKNRGKARQVLGKRHLKISRKRKDHAIKLARCVVQSNDLIAYEDLRIKNMVRNHCLAKSINDASWYQFRVWLE
YFGKVFKRVTVAVNPQYTSLECSSCGEVVKKTLSTRTHVCQCGCVMDRDENAARNILSRGLSTVGHTGTFMLDMSNALGDESSTQAGEILSEQVMSLIKE
SPRL
Blast result :
Comments
ISAsp18 is 71% (TnpB) aa similar to ISTosp1. There are 1 copy in the genome.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.