ISAsp15

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003285 ND Anabaena sp.
Anabaena sp. 90
DNA section
IS Length : 1663 bp

Ends


Left end : CGAAATAACGTAGTATACTGGGGTTAATCGTATTTAAACCCAAGATGAAATACCTAACCCCAGAACAAGTTTACAAACAATTTGGTGATTACCCCAAGAC II struct. : Yes
Right end : GAACCTAAAAGCTCTCCTTACGAGTAAGGTCAATCAGAGAGCGAATTATCAGCAATTAACGCTGTTTGACCTCGGTTAACTACGTTACTTGTGTATCTCT II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TAACTCTCTAATCTTAACCTAACActct

DNA sequence

CGAAATAACGTAGTATACTGGGGTTAATCGTATTTAAACCCAAGATGAAATACCTAACCCCAGAACAAGTTTACAAACAATTTGGTGATTACCCCAAGAC
AACCGCAGAATAGGCTGTCAATGGGAGTATAATAATAAAAGACGTAGTAAGTCTAGAATATGAAACGTATTGTCAAGAATCAGTTAACCGTTACGAGAAT
CGCTATTTTAGGGACTGAAAAACTAAATACTTGGAAATCTAACGAACTTGATTTAATTGCTTTGCGTTTGGGACAACTACGCTCTGACTTGTGGAATGAG
TTCGGGTCGTTGAAAGCCTGGGGTGTGTCTAAATTTGAGATTGACAAACAGCTACGCACATCGAAAGACAAGTATCAATTACCTGCTAAGTTATGGGATT
CAACCCTCTACGATGTAATTGATGATATCCACTTGGTTCAGGCTGCTTGTGTCGAAAAGGTGATGAAATATTTGGGTCAATCGTTCCAATCGTTCCCATC
TAAAAAAGGGGTTTTACAACTTACATTAGAAAGTCGTGAATGGTTGGAACACCCCAAACTTTGCACATTGATTAGAAAGTTTTGGTATCGAGGTCACACA
AAAGTTTGTAACCAAATTATTCTTAGGGCTTTTGATACTAAATTAGATAGCAAAGGCTTAGTGTGGTTGCGTTTTGGTGGACTAACTCCATGTAAAACCC
TCAAGCTACCAACGACTTTACCGACAGAAGTAAAATGTCAATTAAGGCTAATCAAGCGTAATTGCAGATGGGAAATACATTACACGACTGACATCCAAAA
AGCTGAAAAAAAGACTCAAGGTAAAATAATTGGATGTGACCGAGGCTACACAGAAGTGTATGCCACATCATCTAACGATGGTGCTAGATTTTTGGGTAAT
AATTTCGGTAAAATCCAAACAGAAGAGACTGATTATAGAACAGCGAAACAAGTTAAACGCAACAAAATCAGGTCAGTTTTTAATAAATCTATCGCCAAGG
GAAACAGTGCTAAAGCTGATAGGATTAAACGGAATAACCTTGGTAAAATCAAGTGGAATAATCGGGAAACATCCTTTAAAGGTAGGATTAAAACAATCGT
TTTTACAGCCACCCATGACTTGATGTTAAATGCAATCAAGGTAGCTTTTGAAGATTTGACGGAAGCAATAAAAAGCAAAAAGCCCATGAGAAAGCGCATG
AAGAGAAATGTTTCTTCATGGTGCAAGGGGATAATTGCGGATGCTCTCAAACAAGTTTCAACCCGTGTAGGTTGCACGGTTGTTAGTGTGAATTGTGCAT
ATACGTCACAACTTGACTCCAGATTTGCTACCTTAACTGGTAGTAGAAATGGTGACAAGTTTACCGGACATGATGGGGTCGTAATGCACTCAGATACTAA
TGCTGCTGATAACGTTCTAGCAAGAATGAGTGATGTTGAAATCACTCGATACATGAAGCATTCCGATGTAAAAGTAATCTTGCTAGAACGGACGCAGAAG
TTTCGGGACTCCATAGTAATGGAAACCGAGGCGAAAACGGGCAAGGATAAGCCCCAGACTCTAGAACCTAAAAGCTCTCCTTACGAGTAAGGTCAATCAG
AGAGCGAATTATCAGCAATTAACGCTGTTTGACCTCGGTTAACTACGTTACTTGTGTATCTCT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1431 bp476 aa1601590+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRIVKNQLTVTRIAILGTEKLNTWKSNELDLIALRLGQLRSDLWNEFGSLKAWGVSKFEIDKQLRTSKDKYQLPAKLWDSTLYDVIDDIHLVQAACVEK
VMKYLGQSFQSFPSKKGVLQLTLESREWLEHPKLCTLIRKFWYRGHTKVCNQIILRAFDTKLDSKGLVWLRFGGLTPCKTLKLPTTLPTEVKCQLRLIKR
NCRWEIHYTTDIQKAEKKTQGKIIGCDRGYTEVYATSSNDGARFLGNNFGKIQTEETDYRTAKQVKRNKIRSVFNKSIAKGNSAKADRIKRNNLGKIKWN
NRETSFKGRIKTIVFTATHDLMLNAIKVAFEDLTEAIKSKKPMRKRMKRNVSSWCKGIIADALKQVSTRVGCTVVSVNCAYTSQLDSRFATLTGSRNGDK
FTGHDGVVMHSDTNAADNVLARMSDVEITRYMKHSDVKVILLERTQKFRDSIVMETEAKTGKDKPQTLEPKSSPYE

 

Blast result :
Comments
ISAsp15 is 51% (TnpB) aa similar to ISAfe11.
There are 1 complete copy and 1 disrupted copy in the genome.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.