ISfinder Mirror Site

ISAsp14

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_019427	ND	Anabaena sp.	Anabaena sp. 90

DNA section

IS Length : 1794 bp

Ends

Left end : ACAGTTTTGCGTAATTCCCCACCCTCAATGTACTCATAGGGTGGGGATGTAAGCAAGTCAATAACGAAACATCCTGAAAATGTTTTTTACTGTAAGTAAA II struct. : Yes
Right end : GTATGAAGCAACTAGGTCGTGCGAAGCGTCAAAAATCTCAGGCTCCGCTAGGGGATGTAGAAACCCCTGGCTCAAACGAAGTTAGCAAGGGGTAGTTCAT II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TCTGATCTAG	TTTA	CTGCCAGACT	tcat

DNA sequence

ACAGTTTTGCGTAATTCCCCACCCTCAATGTACTCATAGGGTGGGGATGTAAGCAAGTCAATAACGAAACATCCTGAAAATGTTTTTTACTGTAAGTAAA
AGACTTTTGAGGATAGTTGAGTTATTGACAACTATATTTTATTTATTCCAAATTATCAATGTATTTCTCGACTAATTCGCGTATCCAGCCGCCATAAGTC
TGTGACGGTGGTTTTATTTTGTCTATTTGTTCAGCTATCTCTTTCTTGAGAGTAACACTAACCTTGACTCTTGATGCTTCATACTTAGCCGTATAAGCCC
TAGTTTTATCGGCATTTCGTTGTTGCCATTCTTGATGTTTTTTATCTATCATTTTCATTGACACTCTAAGCTCTTGGATTATATAATAGTAGTATATTAT
CAAAGAAGATGAAGGTAATAGTTAATGGCGTTACGTAGAGCAACTTTCAGGCTATATCCTAATAAACAAGTCAGTGAGATGTTGCACTACCACCGCAAAC
TTCATAAAGATTTGTATAATGCTGCTGTATCTAACCGCATTACTTCATATAAAAAGTTTGGTAAAACTGTTTCTTACTTTGAACAACAGAACTGTTTACC
GGATTTCAAAGAAGTCTGGATAGAATATAAAGTAATTAATTCCCAAGCATTACAAGCCACATTAAAACGGGTTGATTTTGCTTTTGGAAGGTTTTTTAAG
GGACTAGGAAAGTACCCTAGATTCAAGCCAATCCGTCATTATTCTGGTTGGACTTATCCATCTTTTACAGGCTGGAAAGTACATAGCACAGGGGATAACG
GCTATTTAGAATTATCAAAGATTGGTCAAATCCAAATGCGTGGTAAGGCTAGACTTTGGGGGCATCCTAAAGCTTTAGATATCGTTAACCGCAATGGTCA
ATGGTATGCTTCCATCGTCTTAGAAATTGATGATACTTTGTTAAAGAATAGCCGCAAAACTGATAATGGTGTTATGGCAATTGATTTAGGTTGTAATGAT
GCAATTGCTTGGACAAATGGTGAAGAAAATGGTTTAGTGGCTGCACCTCGGTTTTTCCGAAAAGCAGAACAAAAAAATCAAGAGTTGGGTAAATCAAAAC
GTCGAAAACGTTCTCCCAATTTCAAAAAGAAAGTTAAGGCTTCTAGAAGGTGGAAGAAAGTTCAGAAGTTAGTTAGTAAACTTTCGAGAAAAGTTGCCAA
CCAAAGACAGAACTTTGTTCACCAAGAAACTACACGAATAATCAGCGGTAATAGCACGGTAGTTACTGAAAAATTAGAAGTCAAAAAAATGAGTGCCAAA
GCTAAGAAAGGCGATGCCCTGAGCTTGTCGAAGGGTAAACGTAAAAAACAAAAGGCAGGACTAAATAAATCAATTCTTGATGTGGGAATGGGGATGATAA
GGGACGCTCTAAAAGCGAAATTATCAGACATTGGTGGCTTATTTGTAGAAGTTCCTACAAAGAAGGTAAAACCATCTCAAACCTGTCCAAAATGCGGAAA
TCAAGAAAAGAAGAGTTTAGCCGCAAGAACTCATGTTTGCCATAACTGCGGATATACCCAACAGCGTGATATCGCCGCCGCAGAAGTAATGCTACTTTGG
TACTCAAATAATCTACAGGGGTTAGGAACTAGCCTCTTAGACGTAGATGATTCTAGCTCTACTTCAAACACCAGCGAACGCAAGAATGCTGGAAGTATGA
AGCAACTAGGTCGTGCGAAGCGTCAAAAATCTCAGGCTCCGCTAGGGGATGTAGAAACCCCTGGCTCAAACGAAGTTAGCAAGGGGTAGTTCAT

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
216 bp	71 aa	358	143	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKMIDKKHQEWQQRNADKTRAYTAKYEASRVKVSVTLKKEIAEQIDKIKPPSQTYGGWIRELVEKYIDNLE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1365 bp	454 aa	425	1789	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MALRRATFRLYPNKQVSEMLHYHRKLHKDLYNAAVSNRITSYKKFGKTVSYFEQQNCLPDFKEVWIEYKVINSQALQATLKRVDFAFGRFFKGLGKYPRF
KPIRHYSGWTYPSFTGWKVHSTGDNGYLELSKIGQIQMRGKARLWGHPKALDIVNRNGQWYASIVLEIDDTLLKNSRKTDNGVMAIDLGCNDAIAWTNGE
ENGLVAAPRFFRKAEQKNQELGKSKRRKRSPNFKKKVKASRRWKKVQKLVSKLSRKVANQRQNFVHQETTRIISGNSTVVTEKLEVKKMSAKAKKGDALS
LSKGKRKKQKAGLNKSILDVGMGMIRDALKAKLSDIGGLFVEVPTKKVKPSQTCPKCGNQEKKSLAARTHVCHNCGYTQQRDIAAAEVMLLWYSNNLQGL
GTSLLDVDDSSSTSNTSERKNAGSMKQLGRAKRQKSQAPLGDVETPGSNEVSKG

Blast result :

Comments

ISAsp14 is 65% (TnpB) aa similar to ISCysp13. The first ORF is a passenger gene annotated as hypothetical protein.
There are 1 complete copy and 2 disrupted copies in the genome.

References

1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.