ISAsp13

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_019427 ND Anabaena sp.
Anabaena sp. 90
DNA section
IS Length : 1602 bp

Ends


Left end : CTTTCCCGACAGAAGCCCCACGCTCACTATACCGGAGCGTGGGATGAATACAATACGCGCGAGTGAGGAATTGCCCAACAGGCGAAATTGAACGCAGTTC II struct. : Yes
Right end : GCTCGGTCAATGTCTTCATAGAAGAGTCGCCGAGAAGCCCACACTCACCTGTATCCCTTACGGGACCGCAAGGCATAAGGGAGTGTGTGGAGTATGTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TATTTTAAGGTTTAGCTATTGTTCATTtcac

DNA sequence

CTTTCCCGACAGAAGCCCCACGCTCACTATACCGGAGCGTGGGATGAATACAATACGCGCGAGTGAGGAATTGCCCAACAGGCGAAATTGAACGCAGTTC
AATCAGGATCGTTGGTCAACGAATGAACATTTTCTGATTTAAGAGAATCTATCCAATCTTCCACTAAGGACGTAATAGTTTTATCTTTGGCGGCGGCGTA
AGTTTGCAATATTCGCATTCTTCTTTCGCTCATTCTCAGGCTTAATCTTTTATTTTTCATAACGGGTAGTTAATGGCTATCCTTTATGTTATATTATAAA
CAGGTCGAAAGGAAACGAAAAATATGAGAACATCTTATCAATACCGATTAAAGCCAACAAGAGAACAAGCCAAAAAAATTGATAAAACATTAGATATGCT
GCGGCATCAGTACAATTATCAACTGGCTCAACGCTTCCATTGGTACGAACAAAACCGTTCTCCTATTGATAGATGTTCCATTTTCGTTTGTCATTTACCA
GAACTCAAGGATAAACCGAATTATTACAGCCAAAAAGCATCATTAACGCAACTTAAAAAAGACCGCCCTTGGTACACAAGTATTCATTCTCAGGTGCTAC
AGGAAGTCCCCAAGAAGGTCGAGATAGCTTTTGACCGATGGTTAAAAGGTGATGTGAATGGTAAAAAATCAGGCAAGCCTAGATTCAAAGGTGCGGGTCA
ATACAAAACTTTTACCTTTCCTCAGTTCAAACGACACCACTTTACTAATAACAAAATTACGCTATCAAAAATAGGTGATATTAAAGTAATTGTTCACCGT
CAAATACCCGGTGGGTTTGATATTAAAACTGTATCTGTCACTAAAAAAGCAGACGGGTATTATATCACTCTAAGCCTTGATGATAAAAGAGTTCCAACGG
TTAAACCTGATTTTAATGCTAATAATATTGTTGGTATTGATGTAGGTTTAATAGATTTTATTGTCACCTCAGACGACGAAAGAATTGCCGCCCCTAAGTT
TTTACGCAAGGCTGAACGCAAATTAAAATCTGCACAGCGTCGCGTATCTCGTAGGAAGAAAGGTTCTAATCGTCGTAAAAAAGCGATAAAAAAACTGGGT
ATTCAACATAAGAAAGTATCAGATACCCGCAAAGATTTCCACTTTAAAACTGCAAACAATCTACTCAAAAAGTACGATGTAGTAGCTGTGGAAAAGTTGA
ATATAAAAGGACTCGCTAGAACATGGTTGGCAAAAAGTATTAACGATGCCGGTTGGGGACAATTTATAACGATTCTTTCAAACAAAGCCGAGAATGCTGG
TTTGAAAGTAATCGCTGTAAATCCAAACGGGACAAGCCAAGAATGCTCTAGTTGCGATGCCCTGAGCTTGCCGAAGGGTGGTCACAAAGTTAAAAAGCCG
CTATCCCAAAGAACGCACAATTGCCCTAACTGTAAGGTCAGTTTGTGCCGGGATTTGAATGCTTCAATTAATATCAAGAACCGTGGGGCGCACGGTCTTA
AAGCTCGGTCAATGTCTTCATAGAAGAGTCGCCGAGAAGCCCACACTCACCTGTATCCCTTACGGGACCGCAAGGCATAAGGGAGTGTGTGGAGTATGTC
AC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1200 bp399 aa3241523+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MRTSYQYRLKPTREQAKKIDKTLDMLRHQYNYQLAQRFHWYEQNRSPIDRCSIFVCHLPELKDKPNYYSQKASLTQLKKDRPWYTSIHSQVLQEVPKKVE
IAFDRWLKGDVNGKKSGKPRFKGAGQYKTFTFPQFKRHHFTNNKITLSKIGDIKVIVHRQIPGGFDIKTVSVTKKADGYYITLSLDDKRVPTVKPDFNAN
NIVGIDVGLIDFIVTSDDERIAAPKFLRKAERKLKSAQRRVSRRKKGSNRRKKAIKKLGIQHKKVSDTRKDFHFKTANNLLKKYDVVAVEKLNIKGLART
WLAKSINDAGWGQFITILSNKAENAGLKVIAVNPNGTSQECSSCDALSLPKGGHKVKKPLSQRTHNCPNCKVSLCRDLNASINIKNRGAHGLKARSMSS

 

Blast result :
Comments
ISAsp13 is 62% (TnpB) aa similar to ISSoc3.
There are 2 copies in the genome.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.