ISAsp12
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_019427 | ND | Anabaena sp. | Anabaena sp. 90 |
DNA section
IS Length : 1519 bp
Ends
Left end : AAAGAAAATGCCGAAAACCCTTGAAGATGTGGCTGACTCAGGAAGCCACATCTGTGCTTCAGACAAGGGATGAAGGCTAGTTGCAGGATTTATCCTGCAT II struct. : Yes
Right end : ACAGGAGTTGAAGCCACTACTCTACTTGGTGCAAGCCTGGAAAAGCCAAGTTTTGACGATGAATGTAGAATCCCCTCGGCTTTAGCCAGGGGAGTGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CAAAGGTGCT | TTAT | ATTTTGAGTA | tcaa |
DNA sequence
AAAGAAAATGCCGAAAACCCTTGAAGATGTGGCTGACTCAGGAAGCCACATCTGTGCTTCAGACAAGGGATGAAGGCTAGTTGCAGGATTTATCCTGCAT
TGACACTGGATCTGGCAGACTGTCTATCCAATCTTCTATTAGTCTAGTTACAGAATATTCTTTTTGTTCGGCTAATAACTTTAGTTTATGGAATCTGCGC
GGTGTCGTTCTAATTCTTATATATTCAGTCTTCATCTTGTCTCCTATTTGTTTCCTATCTATGTTACTATATTAATAGGTCGTAAACAAATAAAGCAATG
ATAGTTCTAGAGTATAAAGTCAAGGGTAAACAGCATCAATATAACGCGATAGATGATGCAATTCGGACTACTCAATTCGTTCGCAATAAAGCGATTAGGT
ATTGGATGGATGCACCACGTGAACTAAAAGTTGATAAGTTTGCCCTGAATAAATACTCTACTGAACAACGGATAGAATTTCCTTTTACTGCTGAGTTAAA
CTCAATGGCTGTGCAATCAGCCGCAGAAAGAGGATGGTTTGCTATATCAAGGTTTTACGACAACTGTAAATCTAAAAAGTCTGGTAAAAAGGGATTCCCC
CGTTTTCAAAAAGATTGCCGTTCCGTTGAATATAAAACATCAGGGTGGAAGCTACATAAAACAAAGCGACGCATCACTTTTACCGACAAGAAAGAGATTG
GTGAACTCAAATTATTAGGCAAATGGGATATTCAATCCTACGAACTTAAAGACATTAAACGAGTGCGCTTAATCCGTCGTGCAGATGGATATTATGCTCA
GGTTTGTATCGGCATTGATGTTGTAGATATTCAACCGAAAACGGGTAATGAAATCGGATTAGATGTTGGTATTGAGTCGTTTTACACTGACTCTAACGGA
CATCATGAACCTAACCCTAAGTTTTTGAGAAAGGCTGAAAAATCAATCAAACATTCTCAAAGACGGATTTACAAAAAGCATAAAGGTTCTAGTTGCAGAA
AAAAAGCTAGAAAACTTTATGCCAAAAAGCACTTAAAAGTAAGTAGGCAACGTATAGAACACGCTAAGAGAATAGCGCGTTGCGTAGTCAAATCTAACGA
TTTAGTCGCCTACGAAGATTTAAGGGTTTCAAATATGGTAAAAAATCATTGTTTAGCAAAATCAATTAGTGATGCTAGTTGGTATTTGTTCCGACAATGG
ATTGAATATTTTGCTGTTAAATTTGACAAGATTGCTATAGCTGTTACTCCACACTATACTTCTCAAAAGTGTTCTAGTTGTGGCGTAATTGTCAAAAAAT
CTCTATCAACTCGCACTCATAATTGTAGTTGTGGATCGTATCTTCACAGAGATACAAATGCAGCAATTAATATTTTAAATCTTGCAAAAGCTAGGGGAGG
GCATCCCCAAAGTAACGCTACAGGAGTTGAAGCCACTACTCTACTTGGTGCAAGCCTGGAAAAGCCAAGTTTTGACGATGAATGTAGAATCCCCTCGGCT
TTAGCCAGGGGAGTGTCAA
TGACACTGGATCTGGCAGACTGTCTATCCAATCTTCTATTAGTCTAGTTACAGAATATTCTTTTTGTTCGGCTAATAACTTTAGTTTATGGAATCTGCGC
GGTGTCGTTCTAATTCTTATATATTCAGTCTTCATCTTGTCTCCTATTTGTTTCCTATCTATGTTACTATATTAATAGGTCGTAAACAAATAAAGCAATG
ATAGTTCTAGAGTATAAAGTCAAGGGTAAACAGCATCAATATAACGCGATAGATGATGCAATTCGGACTACTCAATTCGTTCGCAATAAAGCGATTAGGT
ATTGGATGGATGCACCACGTGAACTAAAAGTTGATAAGTTTGCCCTGAATAAATACTCTACTGAACAACGGATAGAATTTCCTTTTACTGCTGAGTTAAA
CTCAATGGCTGTGCAATCAGCCGCAGAAAGAGGATGGTTTGCTATATCAAGGTTTTACGACAACTGTAAATCTAAAAAGTCTGGTAAAAAGGGATTCCCC
CGTTTTCAAAAAGATTGCCGTTCCGTTGAATATAAAACATCAGGGTGGAAGCTACATAAAACAAAGCGACGCATCACTTTTACCGACAAGAAAGAGATTG
GTGAACTCAAATTATTAGGCAAATGGGATATTCAATCCTACGAACTTAAAGACATTAAACGAGTGCGCTTAATCCGTCGTGCAGATGGATATTATGCTCA
GGTTTGTATCGGCATTGATGTTGTAGATATTCAACCGAAAACGGGTAATGAAATCGGATTAGATGTTGGTATTGAGTCGTTTTACACTGACTCTAACGGA
CATCATGAACCTAACCCTAAGTTTTTGAGAAAGGCTGAAAAATCAATCAAACATTCTCAAAGACGGATTTACAAAAAGCATAAAGGTTCTAGTTGCAGAA
AAAAAGCTAGAAAACTTTATGCCAAAAAGCACTTAAAAGTAAGTAGGCAACGTATAGAACACGCTAAGAGAATAGCGCGTTGCGTAGTCAAATCTAACGA
TTTAGTCGCCTACGAAGATTTAAGGGTTTCAAATATGGTAAAAAATCATTGTTTAGCAAAATCAATTAGTGATGCTAGTTGGTATTTGTTCCGACAATGG
ATTGAATATTTTGCTGTTAAATTTGACAAGATTGCTATAGCTGTTACTCCACACTATACTTCTCAAAAGTGTTCTAGTTGTGGCGTAATTGTCAAAAAAT
CTCTATCAACTCGCACTCATAATTGTAGTTGTGGATCGTATCTTCACAGAGATACAAATGCAGCAATTAATATTTTAAATCTTGCAAAAGCTAGGGGAGG
GCATCCCCAAAGTAACGCTACAGGAGTTGAAGCCACTACTCTACTTGGTGCAAGCCTGGAAAAGCCAAGTTTTGACGATGAATGTAGAATCCCCTCGGCT
TTAGCCAGGGGAGTGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1222 bp | 460 aa | 298 | 1519 | + | No |
AG : TnpB
ORF sequence :
MIVLEYKVKGKQHQYNAIDDAIRTTQFVRNKAIRYWMDAPRELKVDKFALNKYSTEQRIEFPFTAELNSMAVQSAAERGWFAISRFYDNCKSKKSGKKGF
PRFQKDCRSVEYKTSGWKLHKTKRRITFTDKKEIGELKLLGKWDIQSYELKDIKRVRLIRRADGYYAQVCIGIDVVDIQPKTGNEIGLDVGIESFYTDSN
GHHEPNPKFLRKAEKSIKHSQRRIYKKHKGSSCRKKARKLYAKKHLKVSRQRIEHAKRIARCVVKSNDLVAYEDLRVSNMVKNHCLAKSISDASWYLFRQ
WIEYFAVKFDKIAIAVTPHYTSQKCSSCGVIVKKSLSTRTHNCSCGSYLHRDTNAAINILNLAKARGGHPQSNATGVEATTLLGASLEKPSFDDECRIPS
ALARGVS
PRFQKDCRSVEYKTSGWKLHKTKRRITFTDKKEIGELKLLGKWDIQSYELKDIKRVRLIRRADGYYAQVCIGIDVVDIQPKTGNEIGLDVGIESFYTDSN
GHHEPNPKFLRKAEKSIKHSQRRIYKKHKGSSCRKKARKLYAKKHLKVSRQRIEHAKRIARCVVKSNDLVAYEDLRVSNMVKNHCLAKSISDASWYLFRQ
WIEYFAVKFDKIAIAVTPHYTSQKCSSCGVIVKKSLSTRTHNCSCGSYLHRDTNAAINILNLAKARGGHPQSNATGVEATTLLGASLEKPSFDDECRIPS
ALARGVS
Blast result :
Comments
ISAsp12 is 70% (TnpB) aa similar to ISTosp1. The STOP codon is after the end of the IS.
There are 2 complete copies and 1 truncated copy in the genome.
There are 2 complete copies and 1 truncated copy in the genome.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.