ISAsp11
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_019427 | ND | Anabaena sp. | Anabaena sp. 90 |
DNA section
IS Length : 1512 bp
Ends
Left end : AAGAGACTAGGCTGAAAACCCTTGAAGTTGAGTTTTGGAGCGTAGTGGTCAGTGAGCAGTTCGGCAAGCTCACTGTAACACTTGTCGAACTGCGGAAAAA II struct. : Yes
Right end : TACAGGAGATTTGCCCTCTTGGGCGATTGGTGCAAACCTGTCGTCTAATGGCGAGTCAGTGAATGTAGAATCCCCGTGTCTTTAGACCGGGGAGTGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| TAACGTTAAA | TTAT | GAAAAGAAGC | tcaa |
DNA sequence
AAGAGACTAGGCTGAAAACCCTTGAAGTTGAGTTTTGGAGCGTAGTGGTCAGTGAGCAGTTCGGCAAGCTCACTGTAACACTTGTCGAACTGCGGAAAAA
CTCAACTGTCCTTTAGGCAAGGGATGAAAGCCAGATAGAGGCTTCAGCCTCCACTGAAAGTTTTTCCTGTTGACAAGATTATACTTTGTGAGTTAGCATA
GCTTCGTGGTGTTTAGGTCGTAGCCATACTATGGCTAACGCCACGCTACGCTATCGACAGGCTCAGTAACCATGATAATTTATGAGTTCAAAGTCAAAGG
AAAAGATAGGCAGTATCGCGCAATAGATGAGGCTATTCGTGCATCTCAATTCATCCAAAATAAATGTTTAAGATATTGGATGGACAATAAAGGTGCGACG
AAATATGATCTCAATAAATATTGCGCGATTTTGGCATCTGAATTTCCTTTTGCGGATGAACTTAATTCAATGGCAAGACAATCTGCTGCGGAACGTTCTT
GGAGTACAATAGCTCGATTTTACGACAATTGTAAAAAGAAAGTTAAGGGTAAAAAGGGATTTCCAAAATTCAAAAAGAATTGCCGTTCAGTTGAGTACAA
AGTTACCGGCTGGAAGTTATCCGAAACCAGAAAAGCAATTACTTTCTCTGACAAGAAAGGTATAGGTACTCTGAAATTAAAAGGTACTTACGATCTTAAT
TTTTATGATATCAAACAAATTAAGCGAGTTCGTTTAGTCCGTCGGGCTGATGGATATTATGCTCAATTTGCAATTGATGTCAAAATTAGAATTGAATCCC
AACCAACGAATCAAATAGTTGGCTTAGATGTGGGCTTGAAATACTTTATTGCCGATTCAAAAGGTAATGTAGAACCTGCCCCACAATTCTATAGAAAGTC
AGAAAAACAGTTAAGTCGAGCTAATCGTAAAAAATCTAAGAAGTTTGACAGAGATAAGAAAAAAGCTAAACAAACACAATCCAAGAACTATTTAAAAGCT
AAAAATAGGTATGCCCGTAAACATTTAAGAGTAAGTAGGCAGCGAAAAGAATATTGCAAGAGATTAGCATATTCCGTGATCCAGTCTAACGATTTGGTAG
CTTATGAAGACTTAAATATTAAAGGCATGGTAAGAAACCGACATTTAGCTAAATCAATTTCTGATGCTGGCTGGGCAACTTTTCGGCAATGGGTAGAATA
TTTTGGACTCAAATATGGGAAGGCAACAGTTGCTGTTCCTCCCCATAATACTTCTCAAAATTGTGCTAATTGTGGTCAAAAAGTGAAAAAATCCCTATCA
ACCAGAACCCATAATTGTCATCATTGCGGGTTTGTAGAAGATAGAGATATTAATGCTAGTATCAACATCCTAAGATTAGGATTAACTACGGTAGGGATAC
GGTCAGCTACGCTACAGGAGATTTGCCCTCTTGGGCGATTGGTGCAAACCTGTCGTCTAATGGCGAGTCAGTGAATGTAGAATCCCCGTGTCTTTAGACC
GGGGAGTGTCAA
CTCAACTGTCCTTTAGGCAAGGGATGAAAGCCAGATAGAGGCTTCAGCCTCCACTGAAAGTTTTTCCTGTTGACAAGATTATACTTTGTGAGTTAGCATA
GCTTCGTGGTGTTTAGGTCGTAGCCATACTATGGCTAACGCCACGCTACGCTATCGACAGGCTCAGTAACCATGATAATTTATGAGTTCAAAGTCAAAGG
AAAAGATAGGCAGTATCGCGCAATAGATGAGGCTATTCGTGCATCTCAATTCATCCAAAATAAATGTTTAAGATATTGGATGGACAATAAAGGTGCGACG
AAATATGATCTCAATAAATATTGCGCGATTTTGGCATCTGAATTTCCTTTTGCGGATGAACTTAATTCAATGGCAAGACAATCTGCTGCGGAACGTTCTT
GGAGTACAATAGCTCGATTTTACGACAATTGTAAAAAGAAAGTTAAGGGTAAAAAGGGATTTCCAAAATTCAAAAAGAATTGCCGTTCAGTTGAGTACAA
AGTTACCGGCTGGAAGTTATCCGAAACCAGAAAAGCAATTACTTTCTCTGACAAGAAAGGTATAGGTACTCTGAAATTAAAAGGTACTTACGATCTTAAT
TTTTATGATATCAAACAAATTAAGCGAGTTCGTTTAGTCCGTCGGGCTGATGGATATTATGCTCAATTTGCAATTGATGTCAAAATTAGAATTGAATCCC
AACCAACGAATCAAATAGTTGGCTTAGATGTGGGCTTGAAATACTTTATTGCCGATTCAAAAGGTAATGTAGAACCTGCCCCACAATTCTATAGAAAGTC
AGAAAAACAGTTAAGTCGAGCTAATCGTAAAAAATCTAAGAAGTTTGACAGAGATAAGAAAAAAGCTAAACAAACACAATCCAAGAACTATTTAAAAGCT
AAAAATAGGTATGCCCGTAAACATTTAAGAGTAAGTAGGCAGCGAAAAGAATATTGCAAGAGATTAGCATATTCCGTGATCCAGTCTAACGATTTGGTAG
CTTATGAAGACTTAAATATTAAAGGCATGGTAAGAAACCGACATTTAGCTAAATCAATTTCTGATGCTGGCTGGGCAACTTTTCGGCAATGGGTAGAATA
TTTTGGACTCAAATATGGGAAGGCAACAGTTGCTGTTCCTCCCCATAATACTTCTCAAAATTGTGCTAATTGTGGTCAAAAAGTGAAAAAATCCCTATCA
ACCAGAACCCATAATTGTCATCATTGCGGGTTTGTAGAAGATAGAGATATTAATGCTAGTATCAACATCCTAAGATTAGGATTAACTACGGTAGGGATAC
GGTCAGCTACGCTACAGGAGATTTGCCCTCTTGGGCGATTGGTGCAAACCTGTCGTCTAATGGCGAGTCAGTGAATGTAGAATCCCCGTGTCTTTAGACC
GGGGAGTGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1203 bp | 400 aa | 272 | 1474 | + | No |
AG : TnpB
ORF sequence :
MIIYEFKVKGKDRQYRAIDEAIRASQFIQNKCLRYWMDNKGATKYDLNKYCAILASEFPFADELNSMARQSAAERSWSTIARFYDNCKKKVKGKKGFPKF
KKNCRSVEYKVTGWKLSETRKAITFSDKKGIGTLKLKGTYDLNFYDIKQIKRVRLVRRADGYYAQFAIDVKIRIESQPTNQIVGLDVGLKYFIADSKGNV
EPAPQFYRKSEKQLSRANRKKSKKFDRDKKKAKQTQSKNYLKAKNRYARKHLRVSRQRKEYCKRLAYSVIQSNDLVAYEDLNIKGMVRNRHLAKSISDAG
WATFRQWVEYFGLKYGKATVAVPPHNTSQNCANCGQKVKKSLSTRTHNCHHCGFVEDRDINASINILRLGLTTVGIRSATLQEICPLGRLVQTCRLMASQ
KKNCRSVEYKVTGWKLSETRKAITFSDKKGIGTLKLKGTYDLNFYDIKQIKRVRLVRRADGYYAQFAIDVKIRIESQPTNQIVGLDVGLKYFIADSKGNV
EPAPQFYRKSEKQLSRANRKKSKKFDRDKKKAKQTQSKNYLKAKNRYARKHLRVSRQRKEYCKRLAYSVIQSNDLVAYEDLNIKGMVRNRHLAKSISDAG
WATFRQWVEYFGLKYGKATVAVPPHNTSQNCANCGQKVKKSLSTRTHNCHHCGFVEDRDINASINILRLGLTTVGIRSATLQEICPLGRLVQTCRLMASQ
Blast result :
Comments
ISAsp11 is 92% (TnpB) aa similar to ISTosp1. There are 4 copies in the genome with deletion/insertion.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.