ISAsp9
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_019427 | ND | Anabaena sp. | Anabaena sp. 90 |
DNA section
IS Length : 1591 bp
Ends
Left end : GCCAAGATAGGGGTAGAAACAGCTTGATAATTTCTACCCATAACTAGCCTTATGTTACTGTTTGTCCAAGTCTTCAATAAGTTGATCAACAAATTCCCTT II struct. : No
Right end : GACCGTGATGAAGCAGGAAGTAAACACTAAGGTTATCGCTGTCCAGATGTGTCTAGACTTTGGTAATTTTGGGTAAGTTTTATAGAACGGAACGGAAAAA II struct. : No
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GTCCCCATTGTTCA | GATAGGAGAG |
DNA sequence
GCCAAGATAGGGGTAGAAACAGCTTGATAATTTCTACCCATAACTAGCCTTATGTTACTGTTTGTCCAAGTCTTCAATAAGTTGATCAACAAATTCCCTT
AACCGTTGCTGCTGTGTTAGGGACGGTTTTAATTTTTTCTCTGACCCCAGGCAACACCTTAAACTGCAAAGGTGATTTATCGTACGCTACCTGATTTTGT
GGTTTAAATTTAATCCGAGGCATTGCCAAATCCTAATATGTCTGATATACTAGATATCATAGCCCAACATTGAAAGGTCGAATCAATGCTTTTATCATTC
AAAACTGCACTAATTCCAAATAACCGACAGATTACAGCTTTTCGCAAAGCCTCTGGAGTAGCTAGACACGCCTATAACTGGGCAAATGCTCAAATCATAG
ATATTTTAGCTACTCAAAAAGAAGGCGAAAAACTTAAATTACCATCAGCTATTGACTTGCATAAAAAGTTAGTTGCTGAGGTAAAATCTGAGCATATTTG
GTATTACGAAGTTAATAAAAATATCCCTCAGAAGGCGTTAGCTGATTTACGCCAAGCATGGGATAGGTGTTTTAGGAAGACATCAAAACAACCACGATTC
AAGAAAAAAGGTCAACGTGATTCTTTCTATTTAGAATCAGGAACTAAGGCGAAACCGTTAATTAAAAACGATGGTAAAAGAATTAAATTACCGTCAATCG
GCTGGGTACGTTTAGCCATACCTTTACCGATTACAGCAACTCATAATTGCGTGATTTCTAGACAGGCTGATAAATGGTTTATTTCTGTTAAATACGAAGT
TGAAAAACCTCCTATACTTGCAGACAGACCGACCATTGGTGTTGACATTGGTATCAAAGAATTAGCAGTTTGCAGTAATGGTGAAGGATTCTCGAATCCT
AAAGCCTACCGCCATAAGAGTAAACGCATGAAACGTTTACAGCGTAGTGTGAGCAGGAAAATTAAAGGCTCTAATAACCGTAAAAAAGCTGTGAGAAAAT
TAGCTAAATTACACGCCAAAGTTTCTAATATTCGCCAGGATTCTATTCACAAATTAACCTACTATCTAGCTAAAAACCACAGCATAATTAAGATAGAAGA
TTTGCACGTTAAGGCATTTTTGAAGAACCACAAATTAGCAGGTGCAATTGCAGATTGTGGGATGTATGAATTTAAAAGGCAATTAGAGTACAAAACTGAG
AAGTTTGGCAGTGAATTAATCCTTGTGGATAGATTCTTTCCTAGTTCCCAAATATGCTCTAACTGCGGTAATCATCGTCATAAAATGCCATTAAAAAACC
GCGTTTATATTTGTCCTGATTGTGGCTATAAAGCTGACAGGGACTTAAATGCAGCGCGTAATATTGACCGATGGTTTGTGGATATTTTTATCCCTGTAGC
GTAGCGGTTCCTTTTAGGAACTACGGTCAGAAACGGTAAGTTCTACCGAGATTGCCTGTGGAGCGGAAAAACCTCTTAGAAGCCATTCTAAGACCGTGAT
GAAGCAGGAAGTAAACACTAAGGTTATCGCTGTCCAGATGTGTCTAGACTTTGGTAATTTTGGGTAAGTTTTATAGAACGGAACGGAAAAA
AACCGTTGCTGCTGTGTTAGGGACGGTTTTAATTTTTTCTCTGACCCCAGGCAACACCTTAAACTGCAAAGGTGATTTATCGTACGCTACCTGATTTTGT
GGTTTAAATTTAATCCGAGGCATTGCCAAATCCTAATATGTCTGATATACTAGATATCATAGCCCAACATTGAAAGGTCGAATCAATGCTTTTATCATTC
AAAACTGCACTAATTCCAAATAACCGACAGATTACAGCTTTTCGCAAAGCCTCTGGAGTAGCTAGACACGCCTATAACTGGGCAAATGCTCAAATCATAG
ATATTTTAGCTACTCAAAAAGAAGGCGAAAAACTTAAATTACCATCAGCTATTGACTTGCATAAAAAGTTAGTTGCTGAGGTAAAATCTGAGCATATTTG
GTATTACGAAGTTAATAAAAATATCCCTCAGAAGGCGTTAGCTGATTTACGCCAAGCATGGGATAGGTGTTTTAGGAAGACATCAAAACAACCACGATTC
AAGAAAAAAGGTCAACGTGATTCTTTCTATTTAGAATCAGGAACTAAGGCGAAACCGTTAATTAAAAACGATGGTAAAAGAATTAAATTACCGTCAATCG
GCTGGGTACGTTTAGCCATACCTTTACCGATTACAGCAACTCATAATTGCGTGATTTCTAGACAGGCTGATAAATGGTTTATTTCTGTTAAATACGAAGT
TGAAAAACCTCCTATACTTGCAGACAGACCGACCATTGGTGTTGACATTGGTATCAAAGAATTAGCAGTTTGCAGTAATGGTGAAGGATTCTCGAATCCT
AAAGCCTACCGCCATAAGAGTAAACGCATGAAACGTTTACAGCGTAGTGTGAGCAGGAAAATTAAAGGCTCTAATAACCGTAAAAAAGCTGTGAGAAAAT
TAGCTAAATTACACGCCAAAGTTTCTAATATTCGCCAGGATTCTATTCACAAATTAACCTACTATCTAGCTAAAAACCACAGCATAATTAAGATAGAAGA
TTTGCACGTTAAGGCATTTTTGAAGAACCACAAATTAGCAGGTGCAATTGCAGATTGTGGGATGTATGAATTTAAAAGGCAATTAGAGTACAAAACTGAG
AAGTTTGGCAGTGAATTAATCCTTGTGGATAGATTCTTTCCTAGTTCCCAAATATGCTCTAACTGCGGTAATCATCGTCATAAAATGCCATTAAAAAACC
GCGTTTATATTTGTCCTGATTGTGGCTATAAAGCTGACAGGGACTTAAATGCAGCGCGTAATATTGACCGATGGTTTGTGGATATTTTTATCCCTGTAGC
GTAGCGGTTCCTTTTAGGAACTACGGTCAGAAACGGTAAGTTCTACCGAGATTGCCTGTGGAGCGGAAAAACCTCTTAGAAGCCATTCTAAGACCGTGAT
GAAGCAGGAAGTAAACACTAAGGTTATCGCTGTCCAGATGTGTCTAGACTTTGGTAATTTTGGGTAAGTTTTATAGAACGGAACGGAAAAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1119 bp | 372 aa | 286 | 1404 | + | No |
AG : TnpB
ORF sequence :
MLLSFKTALIPNNRQITAFRKASGVARHAYNWANAQIIDILATQKEGEKLKLPSAIDLHKKLVAEVKSEHIWYYEVNKNIPQKALADLRQAWDRCFRKTS
KQPRFKKKGQRDSFYLESGTKAKPLIKNDGKRIKLPSIGWVRLAIPLPITATHNCVISRQADKWFISVKYEVEKPPILADRPTIGVDIGIKELAVCSNGE
GFSNPKAYRHKSKRMKRLQRSVSRKIKGSNNRKKAVRKLAKLHAKVSNIRQDSIHKLTYYLAKNHSIIKIEDLHVKAFLKNHKLAGAIADCGMYEFKRQL
EYKTEKFGSELILVDRFFPSSQICSNCGNHRHKMPLKNRVYICPDCGYKADRDLNAARNIDRWFVDIFIPVA
KQPRFKKKGQRDSFYLESGTKAKPLIKNDGKRIKLPSIGWVRLAIPLPITATHNCVISRQADKWFISVKYEVEKPPILADRPTIGVDIGIKELAVCSNGE
GFSNPKAYRHKSKRMKRLQRSVSRKIKGSNNRKKAVRKLAKLHAKVSNIRQDSIHKLTYYLAKNHSIIKIEDLHVKAFLKNHKLAGAIADCGMYEFKRQL
EYKTEKFGSELILVDRFFPSSQICSNCGNHRHKMPLKNRVYICPDCGYKADRDLNAARNIDRWFVDIFIPVA
Blast result :
Comments
ISAsp9 is 56% aa (TnpB) aa similar to ISCARN56.
There are 1 complete copy and 6 truncated copies in the genome.
There are no secondary structure as expected on the ends of this sequence, but there is an empty site in Nostoc sp. PCC 7107 (CP003548) between positions 5685180 and 5685182. The ends of the sequence are done by Blast results.
There are 1 complete copy and 6 truncated copies in the genome.
There are no secondary structure as expected on the ends of this sequence, but there is an empty site in Nostoc sp. PCC 7107 (CP003548) between positions 5685180 and 5685182. The ends of the sequence are done by Blast results.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.