ISAsp8
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_019427 | ND | Anabaena sp. | Anabaena sp. 90 |
DNA section
IS Length : 1353 bp
Ends
Left end : AACCGCTGTGGTCTTTCGGACATCTTGACAACCCCTATATCTTTCGCTAGGATAACTTCAGTTACAGCGAAACTAAAATGTATCTGACTCAAAAGAATCA II struct. : No
Right end : CACAATGCTTTAAGCGGAGTGTCTAGTGGCTGTTTGGCACAGCCTTTACGAGTGAAAATCTCCTAAGAATCTCAGCGGCTTGGCTCCCTGAGAGTGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GCAGGAGCGG | TTTT | CCCAAACTTG | tcaa |
DNA sequence
AACCGCTGTGGTCTTTCGGACATCTTGACAACCCCTATATCTTTCGCTAGGATAACTTCAGTTACAGCGAAACTAAAATGTATCTGACTCAAAAGAATCA
AATACGGGGACTCAAAGCTAATGAATTTACAGCCCTGAAAGAACTCTGTAGACTGAGTAAGAATCTCTACAATGTAGGACTTTACACAGTTAGGCAGTAT
TACTTTCAAGAGCGGAAACATTTAAAATATGAGTCTAACTATCACTATTGTAAAGGAAATGAGAACTATCGGATGCTTAACACCGATATTGCTCAACAAA
CACTGAAGGTAGTTGATAGAACTTTTCGCAGTTTCTACGGATTGATTACCTCAGTTAAATCTGGATCTTACAGTAGTAAAGTAAGACTACCTCATTATCT
GCCCAAGGAGGGATATTTCCCTTTGATCATGCCCAGAGTCAAAGTTAAAGATGGGAAATTTCGGATTCCTATGTCTACCGCTTTCAAGGCTCAATCTGGT
GAAATCTGGATACCATTTCCTAATAGATTAAACCAAGAGACTCTTAAAGAAGTTCGCATCCTCCCAAAATATAATGGGCGTTTTTTTGAGGTTGAATTCA
TCTCTGAAGCTGAAGAAACACCGATTGAGACTAAAGCCGAAAGTGCTATTGGCATTGACTTGGGACTTGATAATCTAGCGACTTGCATTGACACCAATGG
GGCATCCTTTATTTTGGATGGCAAACCACTTAAATCCTTCAACCAATGGTTCAATAAGGAAAATGCTAGACTGCAATCTATTAAAGATTTGCAGAAAATC
AAGGGAACTACAGAGCGTCAAGCTAGACTAGCTGTTAATCGCAATGCCAAAATTCGAGATTATTTAAATAAAGCTGCTAGGTACGTTATCAACCATTGCA
TTGAGCATCGCATTGATAAGCTGGTAGTTGGATTCAATATTGAGATGAAGCAATCCATCAATATTGGTTCCCGTAATCATCAGAACTTCGTACAGATTCC
CCACAGTAGTTTTAGATTTAAACTGAAAGCTCTCTGTGAGAGATACGGAATCAAGTATGTAGAACAGGAAGAGTCCTATACTAGTAAGGCTAGTTTTTTA
GACAATGATAATATTCCTGTTTTTAATGCCGACAATCCCAAAAAGCACGAATTCTCTGGCAAGCGAATTCAGCGGGGTCTGTATCGTACTCAATTTGGTA
TTTTAGTGAACGCAGACTGCAATGGTGCAGCCAATATTCTCAAAAAAAGTAAGCACAATGCTTTAAGCGGAGTGTCTAGTGGCTGTTTGGCACAGCCTTT
ACGAGTGAAAATCTCCTAAGAATCTCAGCGGCTTGGCTCCCTGAGAGTGTCAA
AATACGGGGACTCAAAGCTAATGAATTTACAGCCCTGAAAGAACTCTGTAGACTGAGTAAGAATCTCTACAATGTAGGACTTTACACAGTTAGGCAGTAT
TACTTTCAAGAGCGGAAACATTTAAAATATGAGTCTAACTATCACTATTGTAAAGGAAATGAGAACTATCGGATGCTTAACACCGATATTGCTCAACAAA
CACTGAAGGTAGTTGATAGAACTTTTCGCAGTTTCTACGGATTGATTACCTCAGTTAAATCTGGATCTTACAGTAGTAAAGTAAGACTACCTCATTATCT
GCCCAAGGAGGGATATTTCCCTTTGATCATGCCCAGAGTCAAAGTTAAAGATGGGAAATTTCGGATTCCTATGTCTACCGCTTTCAAGGCTCAATCTGGT
GAAATCTGGATACCATTTCCTAATAGATTAAACCAAGAGACTCTTAAAGAAGTTCGCATCCTCCCAAAATATAATGGGCGTTTTTTTGAGGTTGAATTCA
TCTCTGAAGCTGAAGAAACACCGATTGAGACTAAAGCCGAAAGTGCTATTGGCATTGACTTGGGACTTGATAATCTAGCGACTTGCATTGACACCAATGG
GGCATCCTTTATTTTGGATGGCAAACCACTTAAATCCTTCAACCAATGGTTCAATAAGGAAAATGCTAGACTGCAATCTATTAAAGATTTGCAGAAAATC
AAGGGAACTACAGAGCGTCAAGCTAGACTAGCTGTTAATCGCAATGCCAAAATTCGAGATTATTTAAATAAAGCTGCTAGGTACGTTATCAACCATTGCA
TTGAGCATCGCATTGATAAGCTGGTAGTTGGATTCAATATTGAGATGAAGCAATCCATCAATATTGGTTCCCGTAATCATCAGAACTTCGTACAGATTCC
CCACAGTAGTTTTAGATTTAAACTGAAAGCTCTCTGTGAGAGATACGGAATCAAGTATGTAGAACAGGAAGAGTCCTATACTAGTAAGGCTAGTTTTTTA
GACAATGATAATATTCCTGTTTTTAATGCCGACAATCCCAAAAAGCACGAATTCTCTGGCAAGCGAATTCAGCGGGGTCTGTATCGTACTCAATTTGGTA
TTTTAGTGAACGCAGACTGCAATGGTGCAGCCAATATTCTCAAAAAAAGTAAGCACAATGCTTTAAGCGGAGTGTCTAGTGGCTGTTTGGCACAGCCTTT
ACGAGTGAAAATCTCCTAAGAATCTCAGCGGCTTGGCTCCCTGAGAGTGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1242 bp | 413 aa | 78 | 1319 | + | No |
AG : TnpB
ORF sequence :
MYLTQKNQIRGLKANEFTALKELCRLSKNLYNVGLYTVRQYYFQERKHLKYESNYHYCKGNENYRMLNTDIAQQTLKVVDRTFRSFYGLITSVKSGSYSS
KVRLPHYLPKEGYFPLIMPRVKVKDGKFRIPMSTAFKAQSGEIWIPFPNRLNQETLKEVRILPKYNGRFFEVEFISEAEETPIETKAESAIGIDLGLDNL
ATCIDTNGASFILDGKPLKSFNQWFNKENARLQSIKDLQKIKGTTERQARLAVNRNAKIRDYLNKAARYVINHCIEHRIDKLVVGFNIEMKQSINIGSRN
HQNFVQIPHSSFRFKLKALCERYGIKYVEQEESYTSKASFLDNDNIPVFNADNPKKHEFSGKRIQRGLYRTQFGILVNADCNGAANILKKSKHNALSGVS
SGCLAQPLRVKIS
KVRLPHYLPKEGYFPLIMPRVKVKDGKFRIPMSTAFKAQSGEIWIPFPNRLNQETLKEVRILPKYNGRFFEVEFISEAEETPIETKAESAIGIDLGLDNL
ATCIDTNGASFILDGKPLKSFNQWFNKENARLQSIKDLQKIKGTTERQARLAVNRNAKIRDYLNKAARYVINHCIEHRIDKLVVGFNIEMKQSINIGSRN
HQNFVQIPHSSFRFKLKALCERYGIKYVEQEESYTSKASFLDNDNIPVFNADNPKKHEFSGKRIQRGLYRTQFGILVNADCNGAANILKKSKHNALSGVS
SGCLAQPLRVKIS
Blast result :
Comments
ISAsp8 is 59% (TnpB) aa similar to ISHwa32. There is no secondary structure on the left end, the clivage site is done by BLAST result.
There are 1 complete copy and 1 truncated copy in the genome.
There are 1 complete copy and 1 truncated copy in the genome.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.