ISAsp8

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_019427 ND Anabaena sp.
Anabaena sp. 90
DNA section
IS Length : 1353 bp

Ends


Left end : AACCGCTGTGGTCTTTCGGACATCTTGACAACCCCTATATCTTTCGCTAGGATAACTTCAGTTACAGCGAAACTAAAATGTATCTGACTCAAAAGAATCA II struct. : No
Right end : CACAATGCTTTAAGCGGAGTGTCTAGTGGCTGTTTGGCACAGCCTTTACGAGTGAAAATCTCCTAAGAATCTCAGCGGCTTGGCTCCCTGAGAGTGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GCAGGAGCGGTTTTCCCAAACTTGtcaa

DNA sequence

AACCGCTGTGGTCTTTCGGACATCTTGACAACCCCTATATCTTTCGCTAGGATAACTTCAGTTACAGCGAAACTAAAATGTATCTGACTCAAAAGAATCA
AATACGGGGACTCAAAGCTAATGAATTTACAGCCCTGAAAGAACTCTGTAGACTGAGTAAGAATCTCTACAATGTAGGACTTTACACAGTTAGGCAGTAT
TACTTTCAAGAGCGGAAACATTTAAAATATGAGTCTAACTATCACTATTGTAAAGGAAATGAGAACTATCGGATGCTTAACACCGATATTGCTCAACAAA
CACTGAAGGTAGTTGATAGAACTTTTCGCAGTTTCTACGGATTGATTACCTCAGTTAAATCTGGATCTTACAGTAGTAAAGTAAGACTACCTCATTATCT
GCCCAAGGAGGGATATTTCCCTTTGATCATGCCCAGAGTCAAAGTTAAAGATGGGAAATTTCGGATTCCTATGTCTACCGCTTTCAAGGCTCAATCTGGT
GAAATCTGGATACCATTTCCTAATAGATTAAACCAAGAGACTCTTAAAGAAGTTCGCATCCTCCCAAAATATAATGGGCGTTTTTTTGAGGTTGAATTCA
TCTCTGAAGCTGAAGAAACACCGATTGAGACTAAAGCCGAAAGTGCTATTGGCATTGACTTGGGACTTGATAATCTAGCGACTTGCATTGACACCAATGG
GGCATCCTTTATTTTGGATGGCAAACCACTTAAATCCTTCAACCAATGGTTCAATAAGGAAAATGCTAGACTGCAATCTATTAAAGATTTGCAGAAAATC
AAGGGAACTACAGAGCGTCAAGCTAGACTAGCTGTTAATCGCAATGCCAAAATTCGAGATTATTTAAATAAAGCTGCTAGGTACGTTATCAACCATTGCA
TTGAGCATCGCATTGATAAGCTGGTAGTTGGATTCAATATTGAGATGAAGCAATCCATCAATATTGGTTCCCGTAATCATCAGAACTTCGTACAGATTCC
CCACAGTAGTTTTAGATTTAAACTGAAAGCTCTCTGTGAGAGATACGGAATCAAGTATGTAGAACAGGAAGAGTCCTATACTAGTAAGGCTAGTTTTTTA
GACAATGATAATATTCCTGTTTTTAATGCCGACAATCCCAAAAAGCACGAATTCTCTGGCAAGCGAATTCAGCGGGGTCTGTATCGTACTCAATTTGGTA
TTTTAGTGAACGCAGACTGCAATGGTGCAGCCAATATTCTCAAAAAAAGTAAGCACAATGCTTTAAGCGGAGTGTCTAGTGGCTGTTTGGCACAGCCTTT
ACGAGTGAAAATCTCCTAAGAATCTCAGCGGCTTGGCTCCCTGAGAGTGTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1242 bp413 aa781319+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MYLTQKNQIRGLKANEFTALKELCRLSKNLYNVGLYTVRQYYFQERKHLKYESNYHYCKGNENYRMLNTDIAQQTLKVVDRTFRSFYGLITSVKSGSYSS
KVRLPHYLPKEGYFPLIMPRVKVKDGKFRIPMSTAFKAQSGEIWIPFPNRLNQETLKEVRILPKYNGRFFEVEFISEAEETPIETKAESAIGIDLGLDNL
ATCIDTNGASFILDGKPLKSFNQWFNKENARLQSIKDLQKIKGTTERQARLAVNRNAKIRDYLNKAARYVINHCIEHRIDKLVVGFNIEMKQSINIGSRN
HQNFVQIPHSSFRFKLKALCERYGIKYVEQEESYTSKASFLDNDNIPVFNADNPKKHEFSGKRIQRGLYRTQFGILVNADCNGAANILKKSKHNALSGVS
SGCLAQPLRVKIS

 

Blast result :
Comments
ISAsp8 is 59% (TnpB) aa similar to ISHwa32. There is no secondary structure on the left end, the clivage site is done by BLAST result.
There are 1 complete copy and 1 truncated copy in the genome.
References
1] Wang,H., Sivonen,K., Rouhiainen,L., Fewer,D.P., Lyra,C., Rantala-Ylinen,A., Vestola,J., Jokela,J., Rantasarkka,K., Li,Z. and Liu,B. (2012) BMC Genomics 13, 613.