ISHna1
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ATYM01000001 | ND | Haloplanus natans | Haloplanus natans DSM 17983 |
DNA section
IS Length : 1456 bp
Ends
Left end : CTGAAAGAGAGGGCGCTAAGCCCCTTTCCTCAAGGAGCGAACGGAACGAAGTGGAGTGAGCGAGTAGGGAGGGGATACAGCGCCCGCACCAGTTGCAAAC II struct. : Yes
Right end : ACATTCGTCCTTCGTCGTGGATGCAAGTCGCGTCGTAGAAGCAGGAAGCTCCGCCCTCAAGGAAGCCGCCTCGGCGGCTGAGTAGGGCGGGGTAGTTCAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CGGGCCCTATGT | TTAG | ACCCTCCAGCCC | tcac |
DNA sequence
CTGAAAGAGAGGGCGCTAAGCCCCTTTCCTCAAGGAGCGAACGGAACGAAGTGGAGTGAGCGAGTAGGGAGGGGATACAGCGCCCGCACCAGTTGCAAAC
ACCCAATCAACACCATTAATGCACTTTATTCCGTACAATTTGACACGTCGTATGAACTACAGCCCTCGCTTCCGCCTGTTGCCTACTGAGGAGCAACGCG
AAGCGATGGACTGGCAACGAAATACCGTGCGACAACTCTACAACCACGCACTCAACGAATTCAACGACATCCCTGAAGACGCGGGCACACTCCGCCAACG
CGTCTGGATGGTGCGTGACACCATCCCCGAACTGAAAGACTGGTGGTCAGACTTGAGACAAGTCTACTCCACCGTCTTGCAGAAATCCGTTGAACGCATC
CGCGACAACATTCAGAACCTCGGGAAACTCAAAGCAAACGGGTACGACGTGGGGTCGTTGAACTGGAAAAAACCGCGTGAGTTCAGGAGTTTCACCTATC
GACAATCGGGCTTCGAACTCGACAAGAAGAGTGGCCCGAACGGTCGAGGCGTTCTCACCCTCACGAAACTCAAAGGAGACACTCGGGAGATCCCAATCCG
TCTCCATCGAGACCTTCCCGACCACGACTCCATCAAAGAAGTCACGCTCAAAAAGGAAGCGAGCGGTGCGTGGTACGTCTCGTTTTGCATCGAGACTGAG
ACTCCAGAGAAACCTGCTGTCGAGAACATCAAACCAGCAGACACTGTGGGGCTCGACCTCGGCGTTCTCAACTTCATCCACGACTCCGAGGGACGCTCAA
TCGGGAAACTTGACTTGGCCGACGAGCGCCGGCGTCTCGAACGTGAGCAACGCTCACTCTCTCGAAAGACCTACCAGTCGAACAACTGGGAGAAACAGCG
GCGGCACGTCGCGAATATTCACGTGCGGATGTCGAACAAAAAGCACGATTACAAGCACAAACTCGCACACGCCTACACCACCCAGTATGATGCCGTACTC
GTTGAGAATCTTAACATCAAAGGGATGCTCGAATCCCCTGAAAACGCGCGTAACAAAGCCGAAGTCGGGTGGCGAGACTTTATCACCATTCTCGAACACC
ACGGTGATAAGAACGGGTGCCACGTCGTGCAAGTCAGTTCACGTGGAACGACTACGGAATGCGCGTCATGTGGAGTGGAGACGCAGAAACCGTTGTGGGT
TCGTGAGCATTCGTGTCCGTCGTGTGGGTTCGAACTCGATAGGGATTGGAATGCGGCTCTCAACGTCCTCTCTTGGGGACTTGACAAACTAGGAGTGGTT
CACTCCGAAGACATGCCTGTGGAGACTGCAACCGCTGTGTCTACCGACGGTGGCGAACATTCGTCCTTCGTCGTGGATGCAAGTCGCGTCGTAGAAGCAG
GAAGCTCCGCCCTCAAGGAAGCCGCCTCGGCGGCTGAGTAGGGCGGGGTAGTTCAC
ACCCAATCAACACCATTAATGCACTTTATTCCGTACAATTTGACACGTCGTATGAACTACAGCCCTCGCTTCCGCCTGTTGCCTACTGAGGAGCAACGCG
AAGCGATGGACTGGCAACGAAATACCGTGCGACAACTCTACAACCACGCACTCAACGAATTCAACGACATCCCTGAAGACGCGGGCACACTCCGCCAACG
CGTCTGGATGGTGCGTGACACCATCCCCGAACTGAAAGACTGGTGGTCAGACTTGAGACAAGTCTACTCCACCGTCTTGCAGAAATCCGTTGAACGCATC
CGCGACAACATTCAGAACCTCGGGAAACTCAAAGCAAACGGGTACGACGTGGGGTCGTTGAACTGGAAAAAACCGCGTGAGTTCAGGAGTTTCACCTATC
GACAATCGGGCTTCGAACTCGACAAGAAGAGTGGCCCGAACGGTCGAGGCGTTCTCACCCTCACGAAACTCAAAGGAGACACTCGGGAGATCCCAATCCG
TCTCCATCGAGACCTTCCCGACCACGACTCCATCAAAGAAGTCACGCTCAAAAAGGAAGCGAGCGGTGCGTGGTACGTCTCGTTTTGCATCGAGACTGAG
ACTCCAGAGAAACCTGCTGTCGAGAACATCAAACCAGCAGACACTGTGGGGCTCGACCTCGGCGTTCTCAACTTCATCCACGACTCCGAGGGACGCTCAA
TCGGGAAACTTGACTTGGCCGACGAGCGCCGGCGTCTCGAACGTGAGCAACGCTCACTCTCTCGAAAGACCTACCAGTCGAACAACTGGGAGAAACAGCG
GCGGCACGTCGCGAATATTCACGTGCGGATGTCGAACAAAAAGCACGATTACAAGCACAAACTCGCACACGCCTACACCACCCAGTATGATGCCGTACTC
GTTGAGAATCTTAACATCAAAGGGATGCTCGAATCCCCTGAAAACGCGCGTAACAAAGCCGAAGTCGGGTGGCGAGACTTTATCACCATTCTCGAACACC
ACGGTGATAAGAACGGGTGCCACGTCGTGCAAGTCAGTTCACGTGGAACGACTACGGAATGCGCGTCATGTGGAGTGGAGACGCAGAAACCGTTGTGGGT
TCGTGAGCATTCGTGTCCGTCGTGTGGGTTCGAACTCGATAGGGATTGGAATGCGGCTCTCAACGTCCTCTCTTGGGGACTTGACAAACTAGGAGTGGTT
CACTCCGAAGACATGCCTGTGGAGACTGCAACCGCTGTGTCTACCGACGGTGGCGAACATTCGTCCTTCGTCGTGGATGCAAGTCGCGTCGTAGAAGCAG
GAAGCTCCGCCCTCAAGGAAGCCGCCTCGGCGGCTGAGTAGGGCGGGGTAGTTCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1290 bp | 429 aa | 152 | 1441 | + | No |
AG : TnpB
ORF sequence :
MNYSPRFRLLPTEEQREAMDWQRNTVRQLYNHALNEFNDIPEDAGTLRQRVWMVRDTIPELKDWWSDLRQVYSTVLQKSVERIRDNIQNLGKLKANGYDV
GSLNWKKPREFRSFTYRQSGFELDKKSGPNGRGVLTLTKLKGDTREIPIRLHRDLPDHDSIKEVTLKKEASGAWYVSFCIETETPEKPAVENIKPADTVG
LDLGVLNFIHDSEGRSIGKLDLADERRRLEREQRSLSRKTYQSNNWEKQRRHVANIHVRMSNKKHDYKHKLAHAYTTQYDAVLVENLNIKGMLESPENAR
NKAEVGWRDFITILEHHGDKNGCHVVQVSSRGTTTECASCGVETQKPLWVREHSCPSCGFELDRDWNAALNVLSWGLDKLGVVHSEDMPVETATAVSTDG
GEHSSFVVDASRVVEAGSSALKEAASAAE
GSLNWKKPREFRSFTYRQSGFELDKKSGPNGRGVLTLTKLKGDTREIPIRLHRDLPDHDSIKEVTLKKEASGAWYVSFCIETETPEKPAVENIKPADTVG
LDLGVLNFIHDSEGRSIGKLDLADERRRLEREQRSLSRKTYQSNNWEKQRRHVANIHVRMSNKKHDYKHKLAHAYTTQYDAVLVENLNIKGMLESPENAR
NKAEVGWRDFITILEHHGDKNGCHVVQVSSRGTTTECASCGVETQKPLWVREHSCPSCGFELDRDWNAALNVLSWGLDKLGVVHSEDMPVETATAVSTDG
GEHSSFVVDASRVVEAGSSALKEAASAAE
Blast result :
Comments
ISHna1 is 86% (TnpB) aa similar to ISHarch10.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Eisen,J., Huntemann,M., Han,J., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2013) Direct submission GenBank.
2] Eisen,J., Huntemann,M., Han,J., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2013) Direct submission GenBank.