ISHla20
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001365 | ND | Halorubrum lacusprofundi | Halorubrum lacusprofundi ATCC 49239 |
DNA section
IS Length : 1529 bp
Ends
Left end : ACAAACCGAGGAGAATACCTGACCCTTCAGGGTCAGGATGAATCCGACAACGCCTCCACAACCCGCCGTCAATAGCAGCCCGGATATTCCACCGTTCTCA II struct. : Yes
Right end : CCAACCTCGGACAGAGGTGGTAGACTGCAAACCCTAATATCCCAATCCAGCGGTGCGGTGCCGTGGGATTCCCGCGTCTTCAGGCGCGGGAGGATGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GCGTTGAGATAT | TTAT | ACGTGCACCAGA | tcaa |
| AGACACAGCCAC | TTAT | GGGAATTCTCGA | tcaa |
DNA sequence
ACAAACCGAGGAGAATACCTGACCCTTCAGGGTCAGGATGAATCCGACAACGCCTCCACAACCCGCCGTCAATAGCAGCCCGGATATTCCACCGTTCTCA
ACCCCAGACTTTACAAGAAAAGCTAGTATAAATGCTTATACAGGCGTTCAGAATGCTGGAGGTCCACCGCACCCATCAGGCAAAAATCCTCAACCACTCA
CAGGGAGAGGAATCGCTTGACCGGCACGGGTGGAGCGCCAGCAAACTCTGGAACGTCGCGAACCACCACTCCCGAAAAGTCTGGAAGGAGACGGGCGAGA
TTCCCGGTCACGGCGACCTCAAAGACGAGTTGAAGACGCATCCAAAATACAACGGACTCCATTCTCAGTCCAGTCAGCGCGTTCTGGAGGAACTCGCTGA
AGCCTTCAACTCGTGGTACGGCTCCGACGACGATCGGGACAATCCACCCGGCTATCGGAAAGAAAACTACTACGACGACCAAGGCCGTCGCGTCCACGAA
GAACACCCGCGTTCCACTGTGACGTGGAAGCAGAACGGCATCAAACACGACACCACAAACAACCGTGTTCGCCTCTCAAAAGGTGCGAACCACAAGGAAC
ACCCGAGAGCGTGGGAATACATCCTTGTCGAATACGAGACACGCCCCGGCGTCACGGTCGAGAACCTACAGCAGGTTCGTGCTGTCTACGACAGCACAAA
GGAACGCTGGGAACTCCACCTCGTCTGCAAAGACGAGATCGAGACACCCAACGCCCCCGGCAACGAGACGGCAGGTGTCGACCTCGGGATCAGCAACTTC
GCCGCCGTCGCCTACAGCACCGAAGACGCCGACCTATACCCCGGCAACCGTCTGAAGCAAGACGGCTACTACTTCCCCAAGGAGATCGCCAAGTGCGACG
ACAGCGGTGGTGACAGGGCAACTCGGCTCCATCACAAGTGGGCGGAACGCCGCACTCACTTCTTCCATTCCTTGGCGAAACACATCGTCGAACGGTGTGT
CGAGAAGGAAGTAGGACGCATCAACGTCGGAGACTTGGAAGGGGTCCGTGAAGATGAGAACGGCAGCTCGAAGAACTGGGGCAAGCACGGGAATCTCGAC
TTACACGGGTGGGCGTTCGACCGCTTCAGCTCGATTCTCGAATACAAGGCGAGAGTCGAGGGAATCGAGGTCTTGGAAGTGTCTGAGCGGGATACGAGCA
AGACATGTTGCACATGCGGGAAAACAGACGACTCACAGCGCGTCCACCGCGGTTTGTACGTCTGTGATGAGTGCGACGCAGCGTTCAACGCCGACGTGAA
CGGGGCGGAGAACATCCGTCTCGACAGCAACGAAAGTAACTCCGAGTCTGCACCCGATTTGGGTGGAGATAGGAGTACCGGCTGGTTGGCACAGCCCGGA
GTCTATCTTCACGACCTCTCTCGAGGATTCCAACCTCGGACAGAGGTGGTAGACTGCAAACCCTAATATCCCAATCCAGCGGTGCGGTGCCGTGGGATTC
CCGCGTCTTCAGGCGCGGGAGGATGTCAA
ACCCCAGACTTTACAAGAAAAGCTAGTATAAATGCTTATACAGGCGTTCAGAATGCTGGAGGTCCACCGCACCCATCAGGCAAAAATCCTCAACCACTCA
CAGGGAGAGGAATCGCTTGACCGGCACGGGTGGAGCGCCAGCAAACTCTGGAACGTCGCGAACCACCACTCCCGAAAAGTCTGGAAGGAGACGGGCGAGA
TTCCCGGTCACGGCGACCTCAAAGACGAGTTGAAGACGCATCCAAAATACAACGGACTCCATTCTCAGTCCAGTCAGCGCGTTCTGGAGGAACTCGCTGA
AGCCTTCAACTCGTGGTACGGCTCCGACGACGATCGGGACAATCCACCCGGCTATCGGAAAGAAAACTACTACGACGACCAAGGCCGTCGCGTCCACGAA
GAACACCCGCGTTCCACTGTGACGTGGAAGCAGAACGGCATCAAACACGACACCACAAACAACCGTGTTCGCCTCTCAAAAGGTGCGAACCACAAGGAAC
ACCCGAGAGCGTGGGAATACATCCTTGTCGAATACGAGACACGCCCCGGCGTCACGGTCGAGAACCTACAGCAGGTTCGTGCTGTCTACGACAGCACAAA
GGAACGCTGGGAACTCCACCTCGTCTGCAAAGACGAGATCGAGACACCCAACGCCCCCGGCAACGAGACGGCAGGTGTCGACCTCGGGATCAGCAACTTC
GCCGCCGTCGCCTACAGCACCGAAGACGCCGACCTATACCCCGGCAACCGTCTGAAGCAAGACGGCTACTACTTCCCCAAGGAGATCGCCAAGTGCGACG
ACAGCGGTGGTGACAGGGCAACTCGGCTCCATCACAAGTGGGCGGAACGCCGCACTCACTTCTTCCATTCCTTGGCGAAACACATCGTCGAACGGTGTGT
CGAGAAGGAAGTAGGACGCATCAACGTCGGAGACTTGGAAGGGGTCCGTGAAGATGAGAACGGCAGCTCGAAGAACTGGGGCAAGCACGGGAATCTCGAC
TTACACGGGTGGGCGTTCGACCGCTTCAGCTCGATTCTCGAATACAAGGCGAGAGTCGAGGGAATCGAGGTCTTGGAAGTGTCTGAGCGGGATACGAGCA
AGACATGTTGCACATGCGGGAAAACAGACGACTCACAGCGCGTCCACCGCGGTTTGTACGTCTGTGATGAGTGCGACGCAGCGTTCAACGCCGACGTGAA
CGGGGCGGAGAACATCCGTCTCGACAGCAACGAAAGTAACTCCGAGTCTGCACCCGATTTGGGTGGAGATAGGAGTACCGGCTGGTTGGCACAGCCCGGA
GTCTATCTTCACGACCTCTCTCGAGGATTCCAACCTCGGACAGAGGTGGTAGACTGCAAACCCTAATATCCCAATCCAGCGGTGCGGTGCCGTGGGATTC
CCGCGTCTTCAGGCGCGGGAGGATGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1314 bp | 437 aa | 153 | 1466 | + | No |
AG : TnpB
ORF sequence :
MLEVHRTHQAKILNHSQGEESLDRHGWSASKLWNVANHHSRKVWKETGEIPGHGDLKDELKTHPKYNGLHSQSSQRVLEELAEAFNSWYGSDDDRDNPPG
YRKENYYDDQGRRVHEEHPRSTVTWKQNGIKHDTTNNRVRLSKGANHKEHPRAWEYILVEYETRPGVTVENLQQVRAVYDSTKERWELHLVCKDEIETPN
APGNETAGVDLGISNFAAVAYSTEDADLYPGNRLKQDGYYFPKEIAKCDDSGGDRATRLHHKWAERRTHFFHSLAKHIVERCVEKEVGRINVGDLEGVRE
DENGSSKNWGKHGNLDLHGWAFDRFSSILEYKARVEGIEVLEVSERDTSKTCCTCGKTDDSQRVHRGLYVCDECDAAFNADVNGAENIRLDSNESNSESA
PDLGGDRSTGWLAQPGVYLHDLSRGFQPRTEVVDCKP
YRKENYYDDQGRRVHEEHPRSTVTWKQNGIKHDTTNNRVRLSKGANHKEHPRAWEYILVEYETRPGVTVENLQQVRAVYDSTKERWELHLVCKDEIETPN
APGNETAGVDLGISNFAAVAYSTEDADLYPGNRLKQDGYYFPKEIAKCDDSGGDRATRLHHKWAERRTHFFHSLAKHIVERCVEKEVGRINVGDLEGVRE
DENGSSKNWGKHGNLDLHGWAFDRFSSILEYKARVEGIEVLEVSERDTSKTCCTCGKTDDSQRVHRGLYVCDECDAAFNADVNGAENIRLDSNESNSESA
PDLGGDRSTGWLAQPGVYLHDLSRGFQPRTEVVDCKP
Blast result :
Comments
ISHla20 is 94% aa similar to ISNma19.
References
1] F. Pfeiffer (2015) Direct submission
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.