ISfinder Mirror Site

ISNpe29

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003373	ND	Natrinema pellirubrum	Natrinema pellirubrum DSM 15624 pNATPE01

DNA section

IS Length : 1597 bp

Ends

Left end : CTGAATGCAGGCGTTAAGCCCCCGTCCTCAAGGAGCAACGCAGGGCGAAGCCTGAGTAGCGTTAACGAGACGTTTCGCGTCTCGTCCGCACACCTGGACG II struct. : Yes
Right end : ACGGGAACCACTTCGGTTCCTGCAAAGCGCGTCGTGGAAGCAGGAAGCCCTACCCTCAAGAACCGAGCTGCGTCAGCGGCGAGTGAGTAGGGCAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CGAGAACGGTCG	TTAG	ATATCGGTCGGT	tcac

DNA sequence

CTGAATGCAGGCGTTAAGCCCCCGTCCTCAAGGAGCAACGCAGGGCGAAGCCTGAGTAGCGTTAACGAGACGTTTCGCGTCTCGTCCGCACACCTGGACG
CGACGCGTTCTGGGGACGCTTTAGGTCGGAGATACAGTGCCGTCATACACTGTTGCAGGCCCACAGGCCTACGCCCACCGAAGCTATTACACGTGTGACG
TGCATAGCGTGCATTACGGTGAAACGGAAGACAATCACCATTCGTGAGGATCAAAACGAGTGGATTGAGGATCAGCACCTCAACCTCTCGTCGTTCGTCC
GTGAGAAATTAGACGAAGTAATCGAAGAACGCGAGTAGCCCTATGTACTACGCCTACAAGTATCGCCTTGAGCCGTCCGACGCCCACCGCGAGGAGTTAG
ACCGCCACCGCGATATCTGTAGGCAAGTCTACAACCACTTCCGCAAGCGACTCGGCGAGTACCGCGAGAACCACGGCGAACTGCCGTCGATGACCACCCT
GCGGTCGGAACTTCCCGACCTCAAAGAGTGGTGGGACGACCTCTCAGATGTGTACTCGCGGACACTCCAAGTCGTTGTGGAACGGCTGTTCGACAACCTC
AAGGGACTCTCCAAGCTCAAAGAGAACGGCTACGGCGTCGGCCAGCTCAAATGGAAGCCGCCACGGGAGTACCGCAGTTTCACCTATAGCCAGTGTGGCT
TCGAGCTCGACAAGAAGGGCGGTCACGCTGTGCTCTCCCTCTCCAAAATCGGCGATATCCCGATACGGCTCCACCGAAACATCCCTGACGACGCCCAACT
CAAGCAGGTCACGGTCAAGAAAGAGCCGACGGGTAAGTGGTTCGCTACCTTCGGCGTCGAAGTTGACCGCGAACCACCTGAGAAACCCGACGAACTCACC
GATGTAGTGGGTATAGACGTAGGGATTCTGAAGTACGCCCACGATACCGACGGTCACGCCGTTGAGTCGCTCAACCTCTCCGACGAGCGCAAACGCTTGG
AACGAGAGCAACGAAAGCTCTCACGGAAGCAACACGGCTCTGCCAACTACGAGAGGCAACGTCGACGCGTTGCGGAGTGTTATGCGGACCTTCGACGCAA
GCGCCGTGACTTCTTGCACAAGCTCTCGAACTACTACGCTCGGGAGTACGACCTTGTGGCGGTCGAAGATTTGAACGTGAAGGGAATGATGGAATCCCCG
TCGAACAGCCGCAACACCGCGTCGGCGGCGTGGCGGACGTTCCTCTCGTTGCTCGAATACAAGTGCGAGCGCGAAGGGACGCATTTCGTGGCGGTCAACC
CGAGAGGGACGACCAAGGAGTGTGCGTCCTGTGGTGTCTCGACGGAGAAGCCGTTGTGGGTCCGTGAACATTCCTGTCCTGCCTGCGGGTTTGAGGTAGA
CAGAGACGCGAACGCGGCGTGGAACATCCTATCTCGCGGTTTGCAAGACGTAGGAATGGGATACTCCGAATCAACGCCTGTGGAGACTGCGCTCCCTACG
GGAACCACTTCGGTTCCTGCAAAGCGCGTCGTGGAAGCAGGAAGCCCTACCCTCAAGAACCGAGCTGCGTCAGCGGCGAGTGAGTAGGGCAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
120 bp	39 aa	219	338	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKRKTITIREDQNEWIEDQHLNLSSFVREKLDEVIEERE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1245 bp	414 aa	343	1587	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MYYAYKYRLEPSDAHREELDRHRDICRQVYNHFRKRLGEYRENHGELPSMTTLRSELPDLKEWWDDLSDVYSRTLQVVVERLFDNLKGLSKLKENGYGVG
QLKWKPPREYRSFTYSQCGFELDKKGGHAVLSLSKIGDIPIRLHRNIPDDAQLKQVTVKKEPTGKWFATFGVEVDREPPEKPDELTDVVGIDVGILKYAH
DTDGHAVESLNLSDERKRLEREQRKLSRKQHGSANYERQRRRVAECYADLRRKRRDFLHKLSNYYAREYDLVAVEDLNVKGMMESPSNSRNTASAAWRTF
LSLLEYKCEREGTHFVAVNPRGTTKECASCGVSTEKPLWVREHSCPACGFEVDRDANAAWNILSRGLQDVGMGYSESTPVETALPTGTTSVPAKRVVEAG
SPTLKNRAASAASE

Blast result :

Comments

ISNpe29 is 91% (TnpB) aa similar to ISHma12. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Pfeiffer F. (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission to GenBanK.