ISHut2
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001687 | ND | Halorhabdus utahensis | Halorhabdus utahensis DSM 12940 |
DNA section
IS Length : 1666 bp
Ends
Left end : CAGTTTCGAGGCGGAGCCTCCGGCCTCAAGTCGAGAGGGACCGGAGCCCCCTCTGACCATTCGAACGGACGCTTCGCGTCCGTGAGAAGAGAGAGAAGAG II struct. : No
Right end : CAGTGGAGGCTCACAGCCTCCACGTCGTCAGACCTCGTCTGACGGAACCCTCAACGAAGCGAGGTCGTCAGACCGAGCGAAGTAGGGTGGGGTAGTTTAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GAGTCCCGGAGC | TCAG | TAGCCGTCCCAG | ttac |
DNA sequence
CAGTTTCGAGGCGGAGCCTCCGGCCTCAAGTCGAGAGGGACCGGAGCCCCCTCTGACCATTCGAACGGACGCTTCGCGTCCGTGAGAAGAGAGAGAAGAG
TTCCGACGCCAGTCGGAAGGACGACGCGAGTAGGCCGGAGAGGACAGAGCGGGACTGAAGGTCCCGCAGGCAGTCGGACGTAGTCCGACGACAGCGAGGC
GCTACGCGCCTCGGGCCGTGCGAGCGGGCGAAGCCCGCACGCAGAAGCCGAAAACTCCGGCACAAACCACGTACCGTCGTCGGCTTTTCTCCCAAATGCT
AATAAATCACCAGCCATACATCTGACGTATGGAGGTGCGGCGCACTGTCCCCGTGAAACTCGACGTGGACAGTGACGACACCGCACTTCTCCGCGAGACA
ATTGCTGAGTTTCTGTGGGCCGCCGACTACGTCGTTGACCACGCTTGGCAGGGCGACTACAAGACGACCAGTAAGGCACAGTTGCAGGAGGAAACCTACG
ACGACGTGCGAGAACAAACGCGGTTGCACGCGAATCTCGTCCAGAACGCTCGCAACAAGGCCGCCGACGCCGTACAGGGCGTCGTCGCCCGCTGGAAACA
GGGCGAGTACGCTGGCAAACCACACTTCTCGAAGCCGACTGTCGTCTACGACAAACGGTGTGCGACCTTCCACGACGACCACGTTTCCCTCGCCACGATA
GACGGACGTATCGAAGTCGAGTACGTCCTGCCGGACGACGACCGCGACACGCCCCACAGCCGGTATCTCGACAGCGACGATCACGAGGTGACGAGTGCGG
AACTTCACAACAAGGACGGCGAGTGGTTCCTTCATCTCCGCACAAAGGCGGAGGTGGAGTCTGACACGCCGAAACAGGCAACGACCGGGCACAGCACAGT
CCTCGGCATTGACCTCGGCGTAAACCAACTCGCCGTCACTTCAACGGGCACGTTCTGGAGCGGTCACGAGTTCGACCACTGGCGGCGCGAGTACGAGAAA
CGGCGCGCCTCACTCCAGCAATGCGGAAGCCGTCACGCTCACGAGAACATCCAGTCGGTCGGGCGCAAAGAGACGGGGCGGTTCAAGATGATGCTCCACC
GGATTGCAAACGGTATCATCGAAGAAGCCGCCGAGAACGGTTGTACGGTCATCGCCTTTGAGGAGCTGACCGGCATCCGTGGCCGGCTCTCTGGTGCCTC
GTGGGGGCACAAGTGGGCATTTGAGCGGTTGTACGAGTACGTCGAATACAAGGCCGAACTCCACGGTATCGATGTAGAGCAGGTGGACCCGGAGAACACC
TCACGGCGTTGTTCACACTGTGGGTTCACGCACCCGGACAACCGCGACAGCGAGGACTTCGAGTGCCTGAAATGCGGGTACGAGAACCACGCCGACTACA
ACGCCGCCAAGAATATCGGACTGCGGTATCTCCGTCGTAACCAAACTGGGGACGGCGGAGGCGCACCCGTAGGCGTGCGCTTGAATCGCGGGACGTTGAA
CGCGAACGGAGAATATGAACCTCCTGCCGGAAATTCCGGCCAGAGCGGGAGTCCACGCGAAAGCTCCAGTGGAGGCTCACAGCCTCCACGTCGTCAGACC
TCGTCTGACGGAACCCTCAACGAAGCGAGGTCGTCAGACCGAGCGAAGTAGGGTGGGGTAGTTTAC
TTCCGACGCCAGTCGGAAGGACGACGCGAGTAGGCCGGAGAGGACAGAGCGGGACTGAAGGTCCCGCAGGCAGTCGGACGTAGTCCGACGACAGCGAGGC
GCTACGCGCCTCGGGCCGTGCGAGCGGGCGAAGCCCGCACGCAGAAGCCGAAAACTCCGGCACAAACCACGTACCGTCGTCGGCTTTTCTCCCAAATGCT
AATAAATCACCAGCCATACATCTGACGTATGGAGGTGCGGCGCACTGTCCCCGTGAAACTCGACGTGGACAGTGACGACACCGCACTTCTCCGCGAGACA
ATTGCTGAGTTTCTGTGGGCCGCCGACTACGTCGTTGACCACGCTTGGCAGGGCGACTACAAGACGACCAGTAAGGCACAGTTGCAGGAGGAAACCTACG
ACGACGTGCGAGAACAAACGCGGTTGCACGCGAATCTCGTCCAGAACGCTCGCAACAAGGCCGCCGACGCCGTACAGGGCGTCGTCGCCCGCTGGAAACA
GGGCGAGTACGCTGGCAAACCACACTTCTCGAAGCCGACTGTCGTCTACGACAAACGGTGTGCGACCTTCCACGACGACCACGTTTCCCTCGCCACGATA
GACGGACGTATCGAAGTCGAGTACGTCCTGCCGGACGACGACCGCGACACGCCCCACAGCCGGTATCTCGACAGCGACGATCACGAGGTGACGAGTGCGG
AACTTCACAACAAGGACGGCGAGTGGTTCCTTCATCTCCGCACAAAGGCGGAGGTGGAGTCTGACACGCCGAAACAGGCAACGACCGGGCACAGCACAGT
CCTCGGCATTGACCTCGGCGTAAACCAACTCGCCGTCACTTCAACGGGCACGTTCTGGAGCGGTCACGAGTTCGACCACTGGCGGCGCGAGTACGAGAAA
CGGCGCGCCTCACTCCAGCAATGCGGAAGCCGTCACGCTCACGAGAACATCCAGTCGGTCGGGCGCAAAGAGACGGGGCGGTTCAAGATGATGCTCCACC
GGATTGCAAACGGTATCATCGAAGAAGCCGCCGAGAACGGTTGTACGGTCATCGCCTTTGAGGAGCTGACCGGCATCCGTGGCCGGCTCTCTGGTGCCTC
GTGGGGGCACAAGTGGGCATTTGAGCGGTTGTACGAGTACGTCGAATACAAGGCCGAACTCCACGGTATCGATGTAGAGCAGGTGGACCCGGAGAACACC
TCACGGCGTTGTTCACACTGTGGGTTCACGCACCCGGACAACCGCGACAGCGAGGACTTCGAGTGCCTGAAATGCGGGTACGAGAACCACGCCGACTACA
ACGCCGCCAAGAATATCGGACTGCGGTATCTCCGTCGTAACCAAACTGGGGACGGCGGAGGCGCACCCGTAGGCGTGCGCTTGAATCGCGGGACGTTGAA
CGCGAACGGAGAATATGAACCTCCTGCCGGAAATTCCGGCCAGAGCGGGAGTCCACGCGAAAGCTCCAGTGGAGGCTCACAGCCTCCACGTCGTCAGACC
TCGTCTGACGGAACCCTCAACGAAGCGAGGTCGTCAGACCGAGCGAAGTAGGGTGGGGTAGTTTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1323 bp | 440 aa | 329 | 1651 | + | No |
AG : TnpB
ORF sequence :
MEVRRTVPVKLDVDSDDTALLRETIAEFLWAADYVVDHAWQGDYKTTSKAQLQEETYDDVREQTRLHANLVQNARNKAADAVQGVVARWKQGEYAGKPHF
SKPTVVYDKRCATFHDDHVSLATIDGRIEVEYVLPDDDRDTPHSRYLDSDDHEVTSAELHNKDGEWFLHLRTKAEVESDTPKQATTGHSTVLGIDLGVNQ
LAVTSTGTFWSGHEFDHWRREYEKRRASLQQCGSRHAHENIQSVGRKETGRFKMMLHRIANGIIEEAAENGCTVIAFEELTGIRGRLSGASWGHKWAFER
LYEYVEYKAELHGIDVEQVDPENTSRRCSHCGFTHPDNRDSEDFECLKCGYENHADYNAAKNIGLRYLRRNQTGDGGGAPVGVRLNRGTLNANGEYEPPA
GNSGQSGSPRESSSGGSQPPRRQTSSDGTLNEARSSDRAK
SKPTVVYDKRCATFHDDHVSLATIDGRIEVEYVLPDDDRDTPHSRYLDSDDHEVTSAELHNKDGEWFLHLRTKAEVESDTPKQATTGHSTVLGIDLGVNQ
LAVTSTGTFWSGHEFDHWRREYEKRRASLQQCGSRHAHENIQSVGRKETGRFKMMLHRIANGIIEEAAENGCTVIAFEELTGIRGRLSGASWGHKWAFER
LYEYVEYKAELHGIDVEQVDPENTSRRCSHCGFTHPDNRDSEDFECLKCGYENHADYNAAKNIGLRYLRRNQTGDGGGAPVGVRLNRGTLNANGEYEPPA
GNSGQSGSPRESSSGGSQPPRRQTSSDGTLNEARSSDRAK
Blast result :
Comments
ISHut2 is 95% aa (TnpB) similar to ISNph16.
References
1] Friedhelm Pfeiffer (2013) Direct submission
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Ovchinnikova,G., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindal,B., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Ovchinnikova,G., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindal,B., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.