ISfinder Mirror Site

ISHla15

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001365	ND	Halorubrum lacusprofundi	Halorubrum lacusprofundi ATCC 49239

DNA section

IS Length : 1835 bp

Ends

Left end : CTGAATTGAGGCGCTAAGCCCCCTTCCTCAGCGAGCGGCGACCGAACGGGAGGCGCGAGCAGGGAGGGGATACAGCGCCGCACAGTTCTCATATACGATT II struct. : Yes
Right end : GGATACAGCAGTATCTGCAAAGCGCGTCCTGGAAGCAGGAAGCCCTACTCTCAAGGAGCGAGCGGCGTCAGCCGTGAGCGAGTAGGGGAGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
AATCTTGACGAT	TTAG	TTGACGGCCTTG	TCAC

DNA sequence

CTGAATTGAGGCGCTAAGCCCCCTTCCTCAGCGAGCGGCGACCGAACGGGAGGCGCGAGCAGGGAGGGGATACAGCGCCGCACAGTTCTCATATACGATT
CGGTGGCAGGCCAACAGATCCTCGTAATCACAATTTTTTTGCAGTAGGGTAGCATAGTATTTGTCGAACCTAATAGTGTACGACCTCGACTCGGGAGCGC
ATTCGACGTTTTCCCTGCACTACCACCTGATACTCACCACGAAGTATCGGCGCGGAGTGCTAACCGAGGAGCGAACCCAATTCATTCACGAGGTCATCGG
CGGGTTCACGGACAACTACGGTGTCGAACTGACGAACCTCGACGGCGAGGACGACCACGTACACATCCTGTTCCGAGCGAAACCAACCACGGACCTCGTG
AAGTTCATCAACACGGTCAAGGGCGCAACCGCTCGCCGTATCCGCAACGAGTACGCGGACGAACTGAAAACCGAACTGTGGGGCGACTCGTTCTGGAACG
ACTCGTACTGCCTCATCTCGACGGGGCAGGTGTCGCTGGATGTGTTGAAACAGTACGTCGAGGACCAACGCGAGTAGAATGTACTACGCCTACAAGTACC
GTCTCAAACCGTCCGACGCCCACCGCGAGGCGTTGGACCGCCACCGAGACATTTGTAGGCAACTGTACAACCACACACTCAACCGCCTCAACGAGTACCA
AGACGAGCACGGTGAACTGCCATCCATGACCACGCTTCGGTCGGAGCTACCCGACCTCAAGAAATGGTGGGACGGCCTCTCGGACGTGTACTCGAAGGTT
CTCCAAACCGTCGTGGAACGGCTGTTTGACAACCTCAAAGGCCTCTCTGCGCTCAAGAAGAACGGCCACGGCGTCGGCCAACTCAAGTGGAAGCCGCCAC
GGGAGTTCCGCAGTTTCACGTACAGTCAGTCTGGCTTCAAGCTCGACAAGAAGGGCGGTCAGACTGTGCTGTCACTCTCGAAACTCGCGGACATACCGAT
TCGGCTTCACCGCGCCATCCCCGACGACGCCACGCTCAAGCAGGTCACGGTCAAGAAGGAACCGACGGGCGAGTGGTTCGCCACGTTCGGCGTCCAAATG
GACCGTGAACCTCCTGAGCCACCTGAGAATCCCGAGAAGTGCGTCGGTATCGACGTGGGGATTCTCAAGTACGCTCACGACACCGACGGCACAGCAGTCG
GGTCGCTCGATCTCACCGACAAACGTGAACGCTTGGAGCGCGAGCAACGGAAACTCTCGCGGAAGCAACACGGGTCGAACAACTACGAGAAGCAACGGCG
ACGAGTCGCGGAGTGTCACGCTAATCTCCGGCAGAAGCGCCGTGACTTCTTGCACAAACTCTCGGCGTATTACGCTCGGGAGTACGATCTCGTGGCGGTC
GAAGACCTGAACGTGAAGGGGATGATGGAGTCGCCGGCGAACAGCCGCAACACCGCCTCCGCCGCGTGGCGGACGTTCCTCTCGTTGCTCGAATACAAGT
GCGAACGGGAGGGGGCACACTTCGTGGCGGTTGATCCGAGAGGGACGACCAAGGAGTGTGCGTCATGTGGCGTCTCGACGGAGAAGCCGTTGTGGGTCCG
TGAACACTCCTGTCCCGCCTGCGGGTTTGAGGCGGACAGGGACGCGAACGCGGCGTGGAACATTCTTTCTCGTGGCCTCGGAGATGTAGGAGTGGGACAC
TCCGAATCAACGCCTGTGGAGACTGCGCTCCCTGTGGATACAGCAGTATCTGCAAAGCGCGTCCTGGAAGCAGGAAGCCCTACTCTCAAGGAGCGAGCGG
CGTCAGCCGTGAGCGAGTAGGGGAGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
402 bp	133 aa	176	577	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MYDLDSGAHSTFSLHYHLILTTKYRRGVLTEERTQFIHEVIGGFTDNYGVELTNLDGEDDHVHILFRAKPTTDLVKFINTVKGATARRIRNEYADELKTE
LWGDSFWNDSYCLISTGQVSLDVLKQYVEDQRE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1242 bp	413 aa	579	1820	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MYYAYKYRLKPSDAHREALDRHRDICRQLYNHTLNRLNEYQDEHGELPSMTTLRSELPDLKKWWDGLSDVYSKVLQTVVERLFDNLKGLSALKKNGHGVG
QLKWKPPREFRSFTYSQSGFKLDKKGGQTVLSLSKLADIPIRLHRAIPDDATLKQVTVKKEPTGEWFATFGVQMDREPPEPPENPEKCVGIDVGILKYAH
DTDGTAVGSLDLTDKRERLEREQRKLSRKQHGSNNYEKQRRRVAECHANLRQKRRDFLHKLSAYYAREYDLVAVEDLNVKGMMESPANSRNTASAAWRTF
LSLLEYKCEREGAHFVAVDPRGTTKECASCGVSTEKPLWVREHSCPACGFEADRDANAAWNILSRGLGDVGVGHSESTPVETALPVDTAVSAKRVLEAGS
PTLKERAASAVSE

Blast result :

Comments

ISHla15 is 99% (TnpA : the transposase) and 97% (TnpB) aa similar to ISHma12.

References

1] F. Pfeiffer (2013) Direct submission
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.