ISfinder Mirror Site

ISHasp1

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP007060	ND	Halobacterium sp.	Halobacterium sp. DL1

DNA section

IS Length : 1601 bp

Ends

Left end : CTGAATCAAGGAGAGAGTCCCGCCGTTCACGGCGGGCGTGAATCCGACACTCCACGGCACAAACCACGTTCGACGCTTATTCAGGGGTATCCTCTCTGAC II struct. : Yes
Right end : CCAACGAGGAGCATACGAACCCGCAAGTTGCCTCCGTGAGCCGGTAAGCGATACCCCCAGCGTGAGGAAACCCCGGCGTTCACGCCGGGGAGGATGTCAT II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GACGTCGGAGAG	CTAC	GTGGTCGCGGGT	tcat
CGCCTTCGCGGG	CTAC	CACGACGTCGTC	tcat

DNA sequence

CTGAATCAAGGAGAGAGTCCCGCCGTTCACGGCGGGCGTGAATCCGACACTCCACGGCACAAACCACGTTCGACGCTTATTCAGGGGTATCCTCTCTGAC
TTTCTTCTCTCCTTCGAGGAGTAAGCCCTTCCACGTCAATCCACGGTGCTTCTTCAATTCCTTCAGTCGTTCGTACTGTTCGTCGTCGTCAAACTCGATG
CGAATTGCAACCATATAATATCACATGAACGGAATATTTATGTGCGTTACGATATAATATGTAGTCGATGAAGCGAGCCAACACGTTCGACGTGATTCCG
CAGTCCCCAGAGGACGAGGAGTTGCTTCGACGGCTGTTGGACGCTTCTGCCGCTCTCTGGAACGAAATCAACTATGAGCGCCGCGAGAACTATGCTGACC
CAGATGGAGACGTATGGGACATCAGCGAGTATCGCGGTCGCTACAGCGGTGTTCTCGGTGCGTCCACGGTTCAGCAGATTGAGCGCAAGAACCGCGAAGC
GTGGAAGTCGTTCTTCAGCCTCAAGAAGAAGGGAGAAGCGAACGGCAAACCCGGATTCTGGGGCAACTCGGAAGACGGGAGAGAACTCCGCACGTACATC
CGCAACACGTCGTACTCCGTTGAGTGGGGTGAATACTCCCGACTCGAAATCCTTGTCGGCAAAGACCTGAAAGACGAGTACGGATTGGGACACCGCGAAC
GCCTCCGGCTCGAACTTCGAGGCGACCCCAACTGGAAAGAGTACGACAAGCAGGGCCGATTAGAGTTGTTCTACGACGAGCAAGCCCAATCGTTCAGGGC
CTTTCAGCCAGTCACAATCAGCGAAGACCATTCTCGACTGGCACAACCACTGGCTTCGGAAGAAGCCGCTCTGGACATTGGTGCGAACAACCTCGTCGCT
TGCACAACCACAACCGGCCAACAACTCCTGTACGAAGGACGTGACCTGTTCGAGCGGTTCCGTGAGACGACGCGAGAAATAGCACGTCTCCAATCACTCT
TGGAAGACGGTCGATACAGCAGTCACCGCATCCGACGCCTGTACGACCGGCGTACCAAGCGGCGTGATCACGCTCAAGACGCACTCGCCCGTGACCTCAT
CGAACGCCTGTACGACGAGGGTGTTTCGACAGTGTACGTTGGAGCGTTGACGGATGTACTGGAAACGCACTGGTCGGTGGAGACGAACGCGAAGACTCAC
AATTTCTGGGCGTTCCGCGCGTTCGTGAACCGACTGGCGTGCACAGCCGAAGAATACGGTATGTCGGTCGAGGTTCGGTCTGAGGCGTGGACGAGTCAGG
AGTGTCCGAACTGCGGTTCAACAGAGGATACGACGCGCCACCACGACACGCTGACGTGTCCGTGCGGATTCGAGGGACACGCCGACCTCACGGCGTCAGA
AACGTTCCTGAGGCGGCAGACGACGGTAACACGGTCGATGGCACGGCCTGTATGCCTCAAGTGGGACGACCATAATTGGTCAGAGTCACCACGCTCAGTT
CCCAACGAGGAGCATACGAACCCGCAAGTTGCCTCCGTGAGCCGGTAAGCGATACCCCCAGCGTGAGGAAACCCCGGCGTTCACGCCGGGGAGGATGTCA
T

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
138 bp	45 aa	214	77	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MVAIRIEFDDDEQYERLKELKKHRGLTWKGLLLEGEKKVREDTPE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1278 bp	426 aa	272	1549	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRANTFDVIPQSPEDEELLRRLLDASAALWNEINYERRENYADPDGDVWDISEYRGRYSGVLGASTVQQIERKNREAWKSFFSLKKKGEANGKPGFWGN
SEDGRELRTYIRNTSYSVEWGEYSRLEILVGKDLKDEYGLGHRERLRLELRGDPNWKEYDKQGRLELFYDEQAQSFRAFQPVTISEDHSRLAQPLASEEA
ALDIGANNLVACTTTTGQQLLYEGRDLFERFRETTREIARLQSLLEDGRYSSHRIRRLYDRRTKRRDHAQDALARDLIERLYDEGVSTVYVGALTDVLET
HWSVETNAKTHNFWAFRAFVNRLACTAEEYGMSVEVRSEAWTSQECPNCGSTEDTTRHHDTLTCPCGFEGHADLTASETFLRRQTTVTRSMARPVCLKWD
DHNWSESPRSVPNEEHTNPQVASVSR

Blast result :

Comments

ISHasp1 is 81% (TnpB) aa similar to ISHhu1. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Pfeiffer, F. (2015) Direct submission
2] Cavicchioli,R., Huntemann,M., Han,J., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2014) Direct submission GenBank.