ISfinder Mirror Site

ISNaoc1

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003929	ND	Natronococcus occultus	Natronococcus occultus SP4

DNA section

IS Length : 1465 bp

Ends

Left end : AGGCAGTACGAGGCGGATGCCCCGATCCTTGAGGTCGGGGAGGAAGCCGACACTCGATGAAACCCACCACGCTCGCTGACAGGGCCGACTCCCCTCCCCA II struct. : Yes
Right end : GGGACGTTGAACGTGAACGGCGAGTACACGCCTTCCGCCTTGCGCGGATAGAACGGGAGTCCACTGACAAGCCTCGGGGCTTGACCCCGAGGCGGTTGAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GCAATGACAGCGT	TCAC	GTCGGATCCACT	tgac

DNA sequence

AGGCAGTACGAGGCGGATGCCCCGATCCTTGAGGTCGGGGAGGAAGCCGACACTCGATGAAACCCACCACGCTCGCTGACAGGGCCGACTCCCCTCCCCA
CACGTAAATTTTTATTAGACAACAATTGTAAATGTGAACCATAGATGGACGACGCGCCACGACGCACTATTCCAATCAAGCTCGACGTGTCCGAAGAGCG
TCGTGGCGACCTCCATCAAACCAAAAGCCAATTCCTGCACTGCGCCAACCGCACCAGCGAGTGGGCGTGGCGCTACGACGACTACTGTGTCACCAGCAAG
AGCAAAGCCGAAAATTCCCTGTACGACGAGTTGCGTGAGGAAACCGACCTCACCGCCAACCTCGTCCAGAAAGGCATCCGCCGCGCCATCGAAGCCGTCG
ATGCAGGCGTCGAAAAACTGCAGCAAGGCGAACAGACGAGTCAACCAGAGTTTGACTCGTGGAGCGTCGTCTACGACAAACGGTCTGCGTCGTTCAACGA
TGATCACGCCACGCTGTCCACGCCGAACGGCAGAGTCACCACTGAGTACGTCCTCCCACCCGAGGACGAACGCGAAGACACGCCGTTCGGCAGGTATTAC
GAGAGTGACGACTGGGATGCGTCGAGCGCCACGCTCCAGTATGACGAGCAGGATGACACGTTCTACCTGCACGTCACGCTGAAGAACCCCGACTACACGG
TTGACAGAACGGAACGCCAAGAAGCCAGTCACGACGATGATGGTGCCGAGAACGGAGTGGTTCTCGGTGTTGACCTGAACGTGACTGGCGCGTTCGCCGT
CACCTCCACGGGAAAGTTCATCGGCAGTGCGGACTACCTCACCCACAAACGCGACCAGTACGAGAAACGCCGTGCCCGCCTGCAACAGACGGGTACACGG
TCCGCTCACCTCACGATTCAGTCCATCGGCAGTCGGTTCTCGGATTGGTCGCTGGACTGGCTCCACAACCGCGCCAACGACCTCATTGCGGAAGCCCAAG
CCACGGATGTAGACGGCATTATTTTTGAAAACCTTGACCACATCCGCGAGAACATTGCGAACGAGTCGAAGTTCCAGCAGTGGACGTATGCGAAGTTCGT
GGAACTCGTGGAGTACAAGGTTGAATCCACGGCGTTGTTCGTGGATACGGTGAATCCTGCGTACACGAGTCAGCGGTGTTCGCACTGTGGGTTTACCCAC
GAGGACAACCGCGACGACAAAGAGTTCGCGTGTCAGGACTGTGGGTATGAGGTGAACGCGGATTACAACGCGGCGAAGAACATTGCGAAACGATACTGCG
GGTATATCCATCGCGGGCAGAAGTCTCGCGGTGGATGGGCCACCAGTCAACTGGCCCTCGAGTCAGGGACGTTGAACGTGAACGGCGAGTACACGCCTTC
CGCCTTGCGCGGATAGAACGGGAGTCCACTGACAAGCCTCGGGGCTTGACCCCGAGGCGGTTGAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1272 bp	423 aa	145	1416	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MDDAPRRTIPIKLDVSEERRGDLHQTKSQFLHCANRTSEWAWRYDDYCVTSKSKAENSLYDELREETDLTANLVQKGIRRAIEAVDAGVEKLQQGEQTSQ
PEFDSWSVVYDKRSASFNDDHATLSTPNGRVTTEYVLPPEDEREDTPFGRYYESDDWDASSATLQYDEQDDTFYLHVTLKNPDYTVDRTERQEASHDDDG
AENGVVLGVDLNVTGAFAVTSTGKFIGSADYLTHKRDQYEKRRARLQQTGTRSAHLTIQSIGSRFSDWSLDWLHNRANDLIAEAQATDVDGIIFENLDHI
RENIANESKFQQWTYAKFVELVEYKVESTALFVDTVNPAYTSQRCSHCGFTHEDNRDDKEFACQDCGYEVNADYNAAKNIAKRYCGYIHRGQKSRGGWAT
SQLALESGTLNVNGEYTPSALRG

Blast result :

Comments

ISNaoc1 is 70% (TnpB) aa similar to ISHvo19.

References

1] Pfeiffer, F. (2015) Direct submission
2] Eisen,J., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J., Klenk,H.-P. and Klenk,H.-P. (2012) Direct submission GenBank.