ISfinder Mirror Site

ISHbo8

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001690	ND	Halogeometricum borinquense	Halogeometricum borinquense DSM 11551

DNA section

IS Length : 1655 bp

Ends

Left end : CCTGAATCAAGGAGAGAGTCCCGACGTTTACGGCGGGCGTGAATCCGACACTCTACGGCACAACCCACGTTCGCCGCTTACTCCGGGGTATCCTCCCTGA II struct. : Yes
Right end : CCCAACGAGGAGCATACGAACCCGCAAGTTGCCTCCGTGGGTCGGTAAACGATACCCCAGCGCGAGGAAACCTCGCCGTGAACGGCGAGGAGGATGTCAT II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CCAATATCTCG	GCTA	AGTGTTCCGACC	tcat

DNA sequence

CCTGAATCAAGGAGAGAGTCCCGACGTTTACGGCGGGCGTGAATCCGACACTCTACGGCACAACCCACGTTCGCCGCTTACTCCGGGGTATCCTCCCTGA
CTTTCTTCTCGCCTTCGAGGAGCAACCCCTTCCACGTCAATCCACGGTGTTTCTTCAGTTCCTTCAGTCGCTCGTACTGCTCGTCGTCATCGAACTCGAT
GCGAACTTCGACCATATAGTAGCACACAAACCACATCTTTATGTGTGTTCCGATGCAATCAGTAGTCGATGAAGCGAGCCAACACGTTCGAGGTGGTTCC
TCTGTCCGACGAGGACGAGGAGTTGCTTCGACGCCTGTTGGACGCTTCTGCCGCTCTCTGGAACGAAATCAACTACGAGCGCCGCGAACACTATGCTGAC
CCAGACGGAGACGTGTGGGACATCAGCGAGTATCGTGGTCGCTACGGCGGTGTTCTCGGGGCGTCCACGGTTCAGCAAATCGAGCGCAAGAACCGCGAAG
CGTGGAAGTCGTTCTTCAGCCTTAGGAAGAAAGGCGAAGCCAACGGAAAACCCGGATACTGGGGCAACGCAGACGAAGGTCGAGAACTCCGCACGTACAT
CCGAAACACGTCGTACTCGGTCGAGTGGGGTGAATACTCCCGGCTCGAAATCCTCGTCGGCAAAGACCTGAAAGACGAGTACGGGTTGGGACACCGCGAA
CGCCTCCGGCTCGAAGTTCGGGGCGACCCGAACTGGACGGAGTATGAGAAGCAGGGTCGATTGGAGTTGTTCTACGACGAGCAAGCACAATCGTTCAGGG
CCTTTCAGCCAGTCACCGTCGATGATTCTCGACTGGCACAACCACTGGCTTCGGAAGAAGCCGCTCTGGATATTGGTGCGAACAACCTCGTCGCCTGCAC
AACCACAACCGGTCAACAACTATTGTACGAAGGACGTGACCTGTTCGACAGGTTCCGCGAGACGACACGAGAAATCGCCCGACTCCAATCACTGTTGGAG
GAAGGTCGATACAGCAGTCACCGAATCCGACGCCTGTACGACCGACGTACCAAGCGACGTGACCACGCCCAAGACGCACTCGCCCGTGACCTCATCGAAC
GCCTCTACGATGAAGGCGTTTCGACGGTGTACGTTGGGGCGTTAACTGACGTGTTGGAAACGCACTGGTCGGTAGAGACGAACGCGAAAACGCACAATTT
CTGGGCGTTCAGGGCGTTCGTGAACCGGGTGGCGTGTACCGCCAAGGAATACGGTATGTCGGTAGAGGTTCGGTCGGAGGCGTGGACGAGTCAGGAGTGT
CCCAACTGTGGTTCGACAGAGGAGACGACGCGCCACAGAGATACGCTGACGTGTCCGTGTGGCTTCGAGGGGCACGCTGACCTCGTGGCGTCAGAGACGT
TCTTGAGACGTCATCAGAACTCTCTGAGTTCTGATTGGCGAACGAGACACTCTGTGTCTCGTCAACGGCAGACGACGGTAACACGGTCGATGGCACGGCC
TGTATGCCTCAAGTGGGACAACCACGAATGGTTGGAGTCATCACGCTCTCCCCGTCCCAACGAGGAGCATACGAACCCGCAAGTTGCCTCCGTGGGTCGG
TAAACGATACCCCAGCGCGAGGAAACCTCGCCGTGAACGGCGAGGAGGATGTCAT

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
138 bp	45 aa	78	215	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MVEVRIEFDDDEQYERLKELKKHRGLTWKGLLLEGEKKVREDTPE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1335 bp	444 aa	269	1603	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRANTFEVVPLSDEDEELLRRLLDASAALWNEINYERREHYADPDGDVWDISEYRGRYGGVLGASTVQQIERKNREAWKSFFSLRKKGEANGKPGYWGN
ADEGRELRTYIRNTSYSVEWGEYSRLEILVGKDLKDEYGLGHRERLRLEVRGDPNWTEYEKQGRLELFYDEQAQSFRAFQPVTVDDSRLAQPLASEEAAL
DIGANNLVACTTTTGQQLLYEGRDLFDRFRETTREIARLQSLLEEGRYSSHRIRRLYDRRTKRRDHAQDALARDLIERLYDEGVSTVYVGALTDVLETHW
SVETNAKTHNFWAFRAFVNRVACTAKEYGMSVEVRSEAWTSQECPNCGSTEETTRHRDTLTCPCGFEGHADLVASETFLRRHQNSLSSDWRTRHSVSRQR
QTTVTRSMARPVCLKWDNHEWLESSRSPRPNEEHTNPQVASVGR

Blast result :

Comments

ISHbo8 is 77% (TnpB) aa similar to ISHhu1. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Pfeiffer, F. (2015) Direct submission
2] Malfatti,S., Tindall,B.J., Schneider,S., Fahnrich,R., Lapidus,A., Labuttii,K., Copeland,A., Glavina Del Rio,T., Nolan,M., Chen,F., Lucas,S., Tice,H., Cheng,J.F., Bruce,D., Goodwin,L., Pitluck,S., Anderson,I., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., D'haeseleer,P., Goker,M., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Chain,P. (2009) Stand Genomic Sci 1 (2), 150-159