ISfinder Mirror Site

ISNagr11

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003377	ND	Natronobacterium gregoryi	Natronobacterium gregoryi SP2

DNA section

IS Length : 1732 bp

Ends

Left end : GCCTGATCGAGGCGCTAATGCCCCTTCCTCAGCGAGCGCCGAAGGCGCGAGCAGGGAGGGGATACAGCGTCCCCAGAAATCTTTGATTTCTGGTGTGCGA II struct. : Yes
Right end : GAAGCGCCCGTTCGCATGGTCCGTGAGACCTTCGGCCTCACGCTACCCTCAAGGAGCGAGCGGCGCAAGCCGCGAGCGAGTAGGGTGGGGAGGAGGTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
ACCGCCGCTGTT	TTAC	TGTGCTGATCGA	tcac

DNA sequence

GCCTGATCGAGGCGCTAATGCCCCTTCCTCAGCGAGCGCCGAAGGCGCGAGCAGGGAGGGGATACAGCGTCCCCAGAAATCTTTGATTTCTGGTGTGCGA
ACGAGACGCTTCGCGTCTCGTCAACGCCGCACGAGTTTCTTGCACTTTTCTACAGCAGGCCCGCAGGCCCACGCTATCGACAGATTGATGAGTATCTACT
TCGTAGATACACTCAATGGATCGGTACGAGGTCGAAGGGCACGAAGTCCTCAAAGCCGAAGTCAAACCGACCGGAAACGGTGCGCACATCTACGTCCCGA
AAGCGTGGACTGGCGCAACTGTCAAAGTCGTCCGTGTCACCGATCCAACCGACGAAGACGAGTAGACTATGCGCTACAACTACAGGTATCGGCTCAACCC
AACTGACGAACTCCACGAACGGTTAGCGTGGACGGTCGATACTTGCCGACAAGTCTACAATCACTTCCTGCACCGACTCAACCGAGAGGACGGCACAACC
GCCTACTCCGAGCAGTCGGTCCTTCCCGATCTCAAACGCGAGTGGACCGAGCTGAAGCAAGTCCATTCGAAGGTGTTGCAGAAGGTCGTACAGCGACTGT
ACGATAATCTCTCGACGCTGCAGGGGCGGAAGGACAACGGCTACCCCGTTGGCGAACTCAAGTGGAAAGGCCCGCAGGAATATTGTTCGATCAAGTACAG
CCAAACCGGCTTCGAACTCAAAAACACGAGTGGCCGTACTGTGCTTTCCCTTTCCAAAATCGGCGATATTCCGATGGTCTACCACCGCGACGTTCCCGAC
GACGCTACGATCAAAGAAATCGTCGTCAAGCAAGAACCAACCGGCGAGTGGTTTGCCGTCCTTGGGATCGAAACCGAGGACGAAGCACCAGAGAAACCCA
AGAAAATCGAGGATTGTGCCGGGATCGACGTGGGCATCCTGAAGTACGCTCACGACACCGACGGCACCGCTGTCGAACGTCCCGACTTGTCCGACGAACG
CGACCGGTTGGAACGCGAGCAACGCAAACTCTCACGGAAAGAACACGGATCGGCGAACTACCGCAAGCAACAGCGAGTCGTCGCCAAACGTCACGCCGAC
CTGCAACAGAAGCGCCAAGACTTCCTTCACAAACTCTCGAACCACTACGCTCGAGAGTACGACCTCGTTGCAGTCGAAGACCTCGACGCGAAGGGGCTGA
TGGAACTCCCGTCGAACAGCCGCAACCGTGCCGGAGCAGCGTGGGGGACATTTTTGCGGATGCTCGAGTACAAATGCGAACGCGAAGGAACGCATTTCGT
GGCCGTCGATCCGGACGAGACGACCAAAGAGTGCGCGGCCTGCGGTGCCGAGTCAGACAAGCCGTTGTGGGTCCGCGAACACTCCTGTCCTTCCTGTGGC
TTCACGGCTGACAGAGACTGGAACGCGGCCTACAACATCTTGGTGCGCGGACTAAAGCAAGTAGGGACGGGCTGTCCCGAATCAACGTCCTCAGAATCGA
AAGATTCTGATGGGCTGCGAAAATCGGCAGACGATTTTCGAACGCCTGCGGAGACTGCGCTCCCTACGGGAACCGATTCGGTTCCTGCAAAGCGCGTCGT
GGAAACAGGAAGCCCCATCTGCTCACGGGCGCGAAGCGCCCGTTCGCATGGTCCGTGAGACCTTCGGCCTCACGCTACCCTCAAGGAGCGAGCGGCGCAA
GCCGCGAGCGAGTAGGGTGGGGAGGAGGTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
150 bp	49 aa	216	365	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDRYEVEGHEVLKAEVKPTGNGAHIYVPKAWTGATVKVVRVTDPTDEDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1290 bp	429 aa	369	1658	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MRYNYRYRLNPTDELHERLAWTVDTCRQVYNHFLHRLNREDGTTAYSEQSVLPDLKREWTELKQVHSKVLQKVVQRLYDNLSTLQGRKDNGYPVGELKWK
GPQEYCSIKYSQTGFELKNTSGRTVLSLSKIGDIPMVYHRDVPDDATIKEIVVKQEPTGEWFAVLGIETEDEAPEKPKKIEDCAGIDVGILKYAHDTDGT
AVERPDLSDERDRLEREQRKLSRKEHGSANYRKQQRVVAKRHADLQQKRQDFLHKLSNHYAREYDLVAVEDLDAKGLMELPSNSRNRAGAAWGTFLRMLE
YKCEREGTHFVAVDPDETTKECAACGAESDKPLWVREHSCPSCGFTADRDWNAAYNILVRGLKQVGTGCPESTSSESKDSDGLRKSADDFRTPAETALPT
GTDSVPAKRVVETGSPICSRARSARSHGP

Blast result :

Comments

ISNagr11 is 74% (TnpB) aa similar to ISNamo25. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Pfeiffer, F. (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank