ISAba25
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP007712 | ND | Acinetobacter baumannii | Acinetobacter baumannii D4 Acinetobacter baumannii LAC-4 Acinetobacter johnsonii plasmid pNDM-XC1 |
DNA section
IS Length : 2491 bp
Ends
IR Length : 10/11
IRL : GTAAGCATCCGGCTAACACAGCCTTACCAAGCGATCCTATAGGCCTTAAT
IRR : GTAAGCGTCCGCTGAATCCCATACCTATTTTTTAATTCGATTTAGGTTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACAAATATC | GGTTAATC | TTTAATAGAA | 8 |
| TAAAGTTCAA | TAAAGTTA | AAACTAATGG | 8 |
DNA sequence
GTAAGCATCCGGCTAACACAGCCTTACCAAGCGATCCTATAGGCCTTAATTTAGTTCCCACCTAAAAACAAGTGGGGACTTAATCCATGAACATGGATAT
ATATTCAGCAACACCTCCTGTTGCTAAAAAACGCCGAAAATACAGCAAAGAATTCAAACTCAGTATTGTCAATGCCTGCAAAAATCCTAATACCTCGATC
GCTTCGGTCGCACTGCAACATAGTATTAATGCCAACCTTGTCAGTCGTTGGATCAGGATCTTCAGCCATCATGAGGGTGCCGTGCAGGATCCTACTCATG
TGAATCCAGCATTTATTGCTTTGCCTTACACTGCTGCAATCAGCCAACCTATTGATGAGAGGATCACGTTGTGTATCACCGTGCCTCATACGAATAATGA
TATTCAGCTAAAATGGCAGACATCAGAAATACCTGCCTTGGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGTTGTCTACTC
AGCCCATGGACATGCGTGCAGGTATGGATACGACCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGATTGGGCATCTGGCTGTGTGCCCGGCGGCTGGAACAGGGCAAATTTCACTGGGCTCAAGTTCAC
CAAGGTGAAACCGTGGCCCTCAGCCCGGAACAGTTACAGGCACTGATCCAAGGTTTGCCCTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTTT
AAACCAGGCTCGACCATTCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTCACCAGGCAGTTGGCGCTGCAGCATCAGTCTCTAGCACAATCAAACCAACAATTGGATGCCAAAGTTCAAC
ATCTTTCTATTCTCAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGCCCAAAAGAATGAACACTTAACGGCAAAACAAATCCA
TCTGTGGGATGAAGCGGTTGAAGAAGATATTGCCGCGGTTGATCTAGAACTGGAACGGCTAAATGCAGATAAAACCAATGCAGCGACACAGAAAGCCAAA
ACCAATAAACCTAAACGTCGACCACTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAATGTGCTTGTGGCTGCCAACTCC
GTCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAAATGGGTCTGTGATCAGTGTGA
CACTCTGACTCAGCAAGCGATGCCCGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTGCTGGTATCGAAGTATGCCGATCAT
TTACCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAGCTTTCTAGATCAACGTTATCTGACTGGATAGGTCGCTGCGGTGTAGAACTGG
AACCTCTGGCCAATGCCTTAAAAGAGGTGGTGCTGCAACAGCGGGTGCTGCATGCAGATGAAACACCGGTCACCATCATGCGGATGGGTGAGAATAATAA
AAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACCACACAGTATAATCCAGTTCAGGCGGTGATCTATGACTTTCAGGATAGTCGTTCAGGCCAGCAT
GCTGAAGAGTTCTTGAAAGGCTGGCAGGGCTATCTAGTCTGTGATGATTACAGTGGTTATAAAGCACGTTTTAAATCAGGCCAGGTGATTGAGGTGGGGT
GCATGGCCCATGCACGTCGTAAATTCCATGAACTGCATGTAACTGGGAAAAGTCAGGTTGCTGAACAGGCCTTAGTGCTGATTCAGAAACTGTATGCGAT
AGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCGGAAGACCGCCGCGAATACCGACAACAGCATAGTCAACCAGTGATGCAACAACTATATGAATGG
CTCAACCAACATCATCTGACAGTGCCATCGAGTTCTCCCACCGCCAAGGCTATCAATTACACTCTGAAGCGTTGGCCAGCCTTAAGCCGCTATCTGGATG
ATGGCAATCTACCGATATGCAACAATTGGGTAGAGAATCAGATGCGTCCCTGGGCGTTGGGACGCAAGAACTGGCTGTTTGCAGGTTCGCTGCGCAGTGG
TCAGCGAGCGGCAAACATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGCTTGGATCCGTATGCCTATTTAAGTGATGTGCTGAAAAGGTTACCGACA
CACAAAGTGACCCAAATTGAAGAATTGCTGCCGCACTGCTGGAAACCTAAATCGAATTAAAAAATAGGTATGGGATTCAGCGGACGCTTAC
ATATTCAGCAACACCTCCTGTTGCTAAAAAACGCCGAAAATACAGCAAAGAATTCAAACTCAGTATTGTCAATGCCTGCAAAAATCCTAATACCTCGATC
GCTTCGGTCGCACTGCAACATAGTATTAATGCCAACCTTGTCAGTCGTTGGATCAGGATCTTCAGCCATCATGAGGGTGCCGTGCAGGATCCTACTCATG
TGAATCCAGCATTTATTGCTTTGCCTTACACTGCTGCAATCAGCCAACCTATTGATGAGAGGATCACGTTGTGTATCACCGTGCCTCATACGAATAATGA
TATTCAGCTAAAATGGCAGACATCAGAAATACCTGCCTTGGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGTTGTCTACTC
AGCCCATGGACATGCGTGCAGGTATGGATACGACCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGATTGGGCATCTGGCTGTGTGCCCGGCGGCTGGAACAGGGCAAATTTCACTGGGCTCAAGTTCAC
CAAGGTGAAACCGTGGCCCTCAGCCCGGAACAGTTACAGGCACTGATCCAAGGTTTGCCCTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTTT
AAACCAGGCTCGACCATTCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTCACCAGGCAGTTGGCGCTGCAGCATCAGTCTCTAGCACAATCAAACCAACAATTGGATGCCAAAGTTCAAC
ATCTTTCTATTCTCAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGCCCAAAAGAATGAACACTTAACGGCAAAACAAATCCA
TCTGTGGGATGAAGCGGTTGAAGAAGATATTGCCGCGGTTGATCTAGAACTGGAACGGCTAAATGCAGATAAAACCAATGCAGCGACACAGAAAGCCAAA
ACCAATAAACCTAAACGTCGACCACTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAATGTGCTTGTGGCTGCCAACTCC
GTCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAAATGGGTCTGTGATCAGTGTGA
CACTCTGACTCAGCAAGCGATGCCCGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTGCTGGTATCGAAGTATGCCGATCAT
TTACCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAGCTTTCTAGATCAACGTTATCTGACTGGATAGGTCGCTGCGGTGTAGAACTGG
AACCTCTGGCCAATGCCTTAAAAGAGGTGGTGCTGCAACAGCGGGTGCTGCATGCAGATGAAACACCGGTCACCATCATGCGGATGGGTGAGAATAATAA
AAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACCACACAGTATAATCCAGTTCAGGCGGTGATCTATGACTTTCAGGATAGTCGTTCAGGCCAGCAT
GCTGAAGAGTTCTTGAAAGGCTGGCAGGGCTATCTAGTCTGTGATGATTACAGTGGTTATAAAGCACGTTTTAAATCAGGCCAGGTGATTGAGGTGGGGT
GCATGGCCCATGCACGTCGTAAATTCCATGAACTGCATGTAACTGGGAAAAGTCAGGTTGCTGAACAGGCCTTAGTGCTGATTCAGAAACTGTATGCGAT
AGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCGGAAGACCGCCGCGAATACCGACAACAGCATAGTCAACCAGTGATGCAACAACTATATGAATGG
CTCAACCAACATCATCTGACAGTGCCATCGAGTTCTCCCACCGCCAAGGCTATCAATTACACTCTGAAGCGTTGGCCAGCCTTAAGCCGCTATCTGGATG
ATGGCAATCTACCGATATGCAACAATTGGGTAGAGAATCAGATGCGTCCCTGGGCGTTGGGACGCAAGAACTGGCTGTTTGCAGGTTCGCTGCGCAGTGG
TCAGCGAGCGGCAAACATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGCTTGGATCCGTATGCCTATTTAAGTGATGTGCTGAAAAGGTTACCGACA
CACAAAGTGACCCAAATTGAAGAATTGCTGCCGCACTGCTGGAAACCTAAATCGAATTAAAAAATAGGTATGGGATTCAGCGGACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 87 | 470 | + | No |
AG : IS66 TnpA
ORF sequence :
MNMDIYSATPPVAKKRRKYSKEFKLSIVNACKNPNTSIASVALQHSINANLVSRWIRIFSHHEGAVQDPTHVNPAFIALPYTAAISQPIDERITLCITVP
HTNNDIQLKWQTSEIPALAELLKALAT
HTNNDIQLKWQTSEIPALAELLKALAT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 336 bp | 111 aa | 467 | 802 | + | No |
AG : IS66 TnpB
ORF sequence :
MIRIDEIWLSTQPMDMRAGMDTTMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAQVHQGETVALSPEQLQALIQGLPWQ
RIGRQQVVTML
RIGRQQVVTML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1584 bp | 527 aa | 877 | 2460 | + | No |
Chemistry : DDE
ORF sequence :
MNTLPDLSQLTHEQLLEFTRQLALQHQSLAQSNQQLDAKVQHLSILNQKYEHELALFKKHKFAQKNEHLTAKQIHLWDEAVEEDIAAVDLELERLNADKT
NAATQKAKTNKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIASPELLSHV
LVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKEVVLQQRVLHADETPVTIMRMGENNKKPKKGYVWAYATTQYNPVQAVIYDF
QDSRSGQHAEEFLKGWQGYLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQVAEQALVLIQKLYAIEAELRKKTDGTAEDRREYRQQHSQP
VMQQLYEWLNQHHLTVPSSSPTAKAINYTLKRWPALSRYLDDGNLPICNNWVENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGLDPYAYLS
DVLKRLPTHKVTQIEELLPHCWKPKSN
NAATQKAKTNKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIASPELLSHV
LVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKEVVLQQRVLHADETPVTIMRMGENNKKPKKGYVWAYATTQYNPVQAVIYDF
QDSRSGQHAEEFLKGWQGYLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQVAEQALVLIQKLYAIEAELRKKTDGTAEDRREYRQQHSQP
VMQQLYEWLNQHHLTVPSSSPTAKAINYTLKRWPALSRYLDDGNLPICNNWVENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGLDPYAYLS
DVLKRLPTHKVTQIEELLPHCWKPKSN
Blast result :
Comments
ISAba25 is 99%(orfA), 100%(orfB) and 95% (orfC, the Transposase) aa similar to ISAba16.
File updated : 2015-04-28 with information provided by Mohammad Hamidian (Ruth Hall team).
File updated : 2015-04-28 with information provided by Mohammad Hamidian (Ruth Hall team).
References
1] Ou HY, Kuang SN, He X, Molgora BM, Ewing PJ, Deng Z, Osby M, Chen W, Xu HH (2015) Sci Rep. 2015 Mar 2;5:8643
2] Mohammad Hamidian and Ruth Hall (2015) Direct submission.
2] Mohammad Hamidian and Ruth Hall (2015) Direct submission.