ISAba25

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007712 ND Acinetobacter baumannii
Acinetobacter baumannii D4
Acinetobacter baumannii LAC-4
Acinetobacter johnsonii plasmid pNDM-XC1
DNA section
IS Length : 2491 bp

Ends


IR Length : 10/11

IRL : GTAAGCATCCGGCTAACACAGCCTTACCAAGCGATCCTATAGGCCTTAAT
IRR : GTAAGCGTCCGCTGAATCCCATACCTATTTTTTAATTCGATTTAGGTTTC

Insertion site


Left flankDirect repeatRight flankDR Length
TACAAATATCGGTTAATCTTTAATAGAA8
TAAAGTTCAATAAAGTTAAAACTAATGG8

DNA sequence

GTAAGCATCCGGCTAACACAGCCTTACCAAGCGATCCTATAGGCCTTAATTTAGTTCCCACCTAAAAACAAGTGGGGACTTAATCCATGAACATGGATAT
ATATTCAGCAACACCTCCTGTTGCTAAAAAACGCCGAAAATACAGCAAAGAATTCAAACTCAGTATTGTCAATGCCTGCAAAAATCCTAATACCTCGATC
GCTTCGGTCGCACTGCAACATAGTATTAATGCCAACCTTGTCAGTCGTTGGATCAGGATCTTCAGCCATCATGAGGGTGCCGTGCAGGATCCTACTCATG
TGAATCCAGCATTTATTGCTTTGCCTTACACTGCTGCAATCAGCCAACCTATTGATGAGAGGATCACGTTGTGTATCACCGTGCCTCATACGAATAATGA
TATTCAGCTAAAATGGCAGACATCAGAAATACCTGCCTTGGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGTTGTCTACTC
AGCCCATGGACATGCGTGCAGGTATGGATACGACCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGATTGGGCATCTGGCTGTGTGCCCGGCGGCTGGAACAGGGCAAATTTCACTGGGCTCAAGTTCAC
CAAGGTGAAACCGTGGCCCTCAGCCCGGAACAGTTACAGGCACTGATCCAAGGTTTGCCCTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTTT
AAACCAGGCTCGACCATTCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTCACCAGGCAGTTGGCGCTGCAGCATCAGTCTCTAGCACAATCAAACCAACAATTGGATGCCAAAGTTCAAC
ATCTTTCTATTCTCAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGCCCAAAAGAATGAACACTTAACGGCAAAACAAATCCA
TCTGTGGGATGAAGCGGTTGAAGAAGATATTGCCGCGGTTGATCTAGAACTGGAACGGCTAAATGCAGATAAAACCAATGCAGCGACACAGAAAGCCAAA
ACCAATAAACCTAAACGTCGACCACTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAATGTGCTTGTGGCTGCCAACTCC
GTCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAAATGGGTCTGTGATCAGTGTGA
CACTCTGACTCAGCAAGCGATGCCCGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTGCTGGTATCGAAGTATGCCGATCAT
TTACCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAGCTTTCTAGATCAACGTTATCTGACTGGATAGGTCGCTGCGGTGTAGAACTGG
AACCTCTGGCCAATGCCTTAAAAGAGGTGGTGCTGCAACAGCGGGTGCTGCATGCAGATGAAACACCGGTCACCATCATGCGGATGGGTGAGAATAATAA
AAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACCACACAGTATAATCCAGTTCAGGCGGTGATCTATGACTTTCAGGATAGTCGTTCAGGCCAGCAT
GCTGAAGAGTTCTTGAAAGGCTGGCAGGGCTATCTAGTCTGTGATGATTACAGTGGTTATAAAGCACGTTTTAAATCAGGCCAGGTGATTGAGGTGGGGT
GCATGGCCCATGCACGTCGTAAATTCCATGAACTGCATGTAACTGGGAAAAGTCAGGTTGCTGAACAGGCCTTAGTGCTGATTCAGAAACTGTATGCGAT
AGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCGGAAGACCGCCGCGAATACCGACAACAGCATAGTCAACCAGTGATGCAACAACTATATGAATGG
CTCAACCAACATCATCTGACAGTGCCATCGAGTTCTCCCACCGCCAAGGCTATCAATTACACTCTGAAGCGTTGGCCAGCCTTAAGCCGCTATCTGGATG
ATGGCAATCTACCGATATGCAACAATTGGGTAGAGAATCAGATGCGTCCCTGGGCGTTGGGACGCAAGAACTGGCTGTTTGCAGGTTCGCTGCGCAGTGG
TCAGCGAGCGGCAAACATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGCTTGGATCCGTATGCCTATTTAAGTGATGTGCTGAAAAGGTTACCGACA
CACAAAGTGACCCAAATTGAAGAATTGCTGCCGCACTGCTGGAAACCTAAATCGAATTAAAAAATAGGTATGGGATTCAGCGGACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa87470+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MNMDIYSATPPVAKKRRKYSKEFKLSIVNACKNPNTSIASVALQHSINANLVSRWIRIFSHHEGAVQDPTHVNPAFIALPYTAAISQPIDERITLCITVP
HTNNDIQLKWQTSEIPALAELLKALAT

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
336 bp111 aa467802+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRIDEIWLSTQPMDMRAGMDTTMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAQVHQGETVALSPEQLQALIQGLPWQ
RIGRQQVVTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1584 bp527 aa8772460+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNTLPDLSQLTHEQLLEFTRQLALQHQSLAQSNQQLDAKVQHLSILNQKYEHELALFKKHKFAQKNEHLTAKQIHLWDEAVEEDIAAVDLELERLNADKT
NAATQKAKTNKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIASPELLSHV
LVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKEVVLQQRVLHADETPVTIMRMGENNKKPKKGYVWAYATTQYNPVQAVIYDF
QDSRSGQHAEEFLKGWQGYLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQVAEQALVLIQKLYAIEAELRKKTDGTAEDRREYRQQHSQP
VMQQLYEWLNQHHLTVPSSSPTAKAINYTLKRWPALSRYLDDGNLPICNNWVENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGLDPYAYLS
DVLKRLPTHKVTQIEELLPHCWKPKSN

 

Blast result :
Comments
ISAba25 is 99%(orfA), 100%(orfB) and 95% (orfC, the Transposase) aa similar to ISAba16.
File updated : 2015-04-28 with information provided by Mohammad Hamidian (Ruth Hall team).
References
1] Ou HY, Kuang SN, He X, Molgora BM, Ewing PJ, Deng Z, Osby M, Chen W, Xu HH (2015) Sci Rep. 2015 Mar 2;5:8643
2] Mohammad Hamidian and Ruth Hall (2015) Direct submission.