TnAs1

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 6694 bp

Ends


IR Length : 34/41

IRL : GGGGACGATAGAGAATTCGGAAAAAATCGTACGCTAAGCTTCGCGAGTTC
IRR : GGGGAGCCCGCAGAATTCGGAAAAAATCGTACGCTAAGGTTTTCCGGGCA

Insertion site


Left flankDirect repeatRight flankDR Length
TCTCGCTGAATATTAGCGTA0

DNA sequence

GGGGACGATAGAGAATTCGGAAAAAATCGTACGCTAAGCTTCGCGAGTTCTTGTAACCAATTCATTGTTTTTTCAATGGATGAAAGAAAATGGCGAAAGC
TATTTGACAAACAAATCGGGATCTTATAACTTACCTACCAAGTAGTCGGTAGATGAAATAAAGCCATGAAACTCAATTCAGATCTTTCGCAGCGTGTTGT
CGTCGAGCCTTCAAGCTTACAGTGGGTCGATTCGCCAGCGACCGGGATTCAACGCCAGTTACTGGAGCGTGATGGCGACGAAGTGGCGCGTGCCACCTCG
ATCGTGCGATACGCGCCGGGCTCCGCTTTTGAAAATCATAAGCACGACTTGGGCGAGGAGATTCTGGTCCTGGACGGCGAATTCAGCGACGAATCCGGGA
CCTTCGGGCCTGGGACTTACATCAAGAATCCACCAGGTTCTTCGCATGCACCGAGCTCCGCGACGGGTTGCACGTTGTTCGTCAAGCTGCGCCATCTTGA
TCTAGCCGACAGCCAACGCACCGTGGTCGACACCCGTAACGGCCCCTGGTTTCCAGGCCTCGTGCCGGGTCTGTCGGTGATGCCACTTTCTGAGTTCGAC
ACACAACATACGGCTTTGGTGCGCTGGGCCCCGGGGACACGATTCAATTCGCATCGTCACTACGGCGGTGAAGAAATCTATGTGTTGGAAGGCGTCTTTG
AGGATGAGTTCGGAAGCTACCCTACCGGCACATGGATGCGCAGTCCGCATTTGAGTGCTCACCGTCCCTTCAGCAATACAGGCTGCACCATCCTGGTCAA
GACAGGCCACCTGCCAGTCGCCGAATCAAATGAGGCGCTTGCATGAAGCATTTCTCCGAAATCTCGCCGACCGCCGAGCGGGTGGTCGATGCCGCTGAAG
GGTTGGTACAGCAGCACGGCTACAACGGCTTCTCATACGATGACGTGGCCCAGTTGGTAGGCATCAAAAAACCAAGTATCCACCACCACTTTCCCAAGAA
GGGTGAACTCGTGGCCGTGGTCGCACAGCGCTACACGCACCGGTTTCGTGAGGAGTTGCTGAGCATCGAAGGGCAGCATGCGAAAGCGCCTGACAGGCTA
ACTGCGTATGCGGCACTTTTCGAACGCACCTTCGCCAAGGACCGGCGCCTTTGTGTCTGCGGCATGTTGGGTGCCGAGTCGGACTCGCTGCCGGACGCCG
TTGTGAGCGAGGTCGAGCGATTCTTTAAAGTCAATCTCGACTGGTTGACTCTTGTCGTTGCCGATGGTCAGCGTGCCGCGCTGATCACCTCGAATTCCAC
CCCAGAGGCTCTCGCAGAGGCATTCTTGTGCGCGTTGGAAGGCTCGATGATGGTGGGCCGTGGCATGCGGTCGTCACGCGGACCGGCCGAAGTTGGAAAC
ACTTTTCTTTCCACCGTGTTGACCTGAGGTCGGCACTTTTTTTGCAACACAACCCTACCATTTAGTTGGTAGTTAAGGAGATTATTATGAGTACTGAATT
TAATGGCAAGAAGTTGTTGGTCATCGGCGGCACTAGCGGGATGGGACTGCAAACTGCCCGCATGGTTCTTGAGCAAGGCGGAAGCGTCGTCATCGTCGGT
CACCGTGAAGACAAGGCCGAAGAGGCCCGCAAGGCGCTGTCGTCGTTGGGCACCGTGACCGCCCTGACTGCCGATCTCTCGCGAGCCGAAGATGTGAAGC
GACTCCTGCATACCATCGATGAACACCACAAGGACATCAATCTTCTGGTCAACGCGGCTGGGGTGTTCTTCCCGAAGGCGTTTCTTGAGCACACGGAAAG
TGATTACGAACAATATTTGACGTTGAATAAAGCGTTCTTCTTCATCACACAAAAAGTCGTGGCCAATCTCGTGGCCAGCGAGCGTCCCGGCGCCATTGTG
AACATCGGCTCGATGTGGGGCAAGCAGGCGATTGCGGCTACGCCGTCGTCCGCGTATTCGATGGCAAAAGCAGGTTTGCATTCGCTGACCCAGCACCTTG
CGATGGAGCTGGCATCCAAACAAATCAGGGTCAATGCCGTTTCTCCGGCGGTTGTCGAAACGCCGATTTACGAGGGATTCATACCCAAGGCCGAGGTTCA
TGGCGCCTTACAGGGATTCAACAGCTTCCACCCAATCGGCAGGGTTGGGACGCCACAAGACGTCGCCGAGGTCATCCTCTTCCTGTTGTCGGACAAGGCA
GCATGGGTGACGGGCGCAATCTGGGACGTTGACGGCGGCGTGATGGCCGGGCGTAACACATAATGGGGGAACGAGAAATGAACACAAACATTAAAGGAAC
TCCCGGCAACGGGATCAACGTCGGCGCATTGCGTGAGTTTGCAGATCAGGTCGCAGCAAAGCCCGCCGCAGGCATCGCGACGTTCGGCGTCGTGACAACC
TGGGAGGGTGGCACCCGGACGCGTGCGCGAACCATGCCGCTCGTACTAGGTGACACGGCCTTGGCGCGCGGCTTTGTCATCGACGCGGACGAACCGGCAG
AATTGCTCGGTACCGACACGGCCGCTAATCCACAAGAATTGATCCTGGCTGCGTTGAACGCATGCATGACTGCAACCTACGCGGCAAATGCGGCGGCAAT
GAACATCGAGTTGCAATCGCTGACTATCCGCACGAAGGGAAGCCTCGATCTGAGAGGTTTTCTTGGAATCGATCCTGGGATCAATCCAGGGTACGACCAG
GTCGAGTATGAGGTCGAAATGGAGTCGTCGGCGGATTCGGCGGCGCTGAAAGCATTGCACGCGCAGGTGCAGCGAACATCGCCGAATTACCACAACTTCG
CGAGAGCCATTGATCTCAAGGCCAAGCTGACCATTCTCCAATGATCGAGTCAGTGTTTCCCGTGGTTGGCTGACGGGAGGCCAAACCAGATGCGCTATGA
CGGAAGGTTCAACCTTCCGTCATCACGTTCTCTGGAATACGCAGTGTCAATTTGAGGTATACCCTAACTTGATGTCAGGCAGGGCCGCGCCGCTTCGTCA
GAATAGAGTCTGCTTTCCCATTTTTTGACACATGCCCGCGAAGGTTATAGATTTCAGCCTGACAGAAATGGGCTTTGAGGCACAACGGAACAGAAAGTGC
ACTTAAGCCGCCTTCAACCAAGGAGACATCGTGCAGGGGCACCGCATCGGCTACGTCCGGGTCAGCAGCTTTGACCAGAACCCGGAACGCCAGCTGGAAC
AAACCCAGGTGAGCAAGGTGTTCACCGACAAGGCATCGGGCAAGGACACCCAGCGCCCCCAGCTCGAAGCGCTGCTGAGCTTCGTCCGCGAAGGCGATAC
AGTGGTGGTGCACAGCATGGATCGGCTGGCCCGCAACCTCGATGACCTGCGTCGCTTGGTACAGAAGCTGACTCAGCGCGGCGTGCGCATCGAGTTCCTG
AAGGAGGGCCTGGTGTTCACTGGCGAGGACTCGCCGATGGCCAACCTGATGCTGTCGGTGATGGGGGCCTTCGCTGAGTTCGAGCGCGCCCTGATCCGCG
AGCGGCAGCGTGAGGGCATCGCCTTGGCCAAGCAGCGTGGCGCGTACCGGGGCCGCAAGAAAGCCCTGTCCGATGAGCAGGCTGCTACCCTGCGGCAGCG
AGCGACGGCCGGCGAGCCCAAGGCGCAGCTTGCCCGCGAGTTCAACATCAGCCGGGAAACCCTCTACCAGTACCTCCGCACGGACGACTGACACATGCCG
CGTCGCTTGATCCTCTCGGCCACGGAGCGGGACACCCTGCTTGCGCTGCCGGAAAGCCAGGATGACCTGATCCGCTACTACACCTTCAACGACTCCGACC
TGTCGCTGATCCGCCAGCGACGCGGCGACGCCAACCGCCTCGGCTTCGCCGTGCAGCTCTGCCTGCTGCGCTACCCCGGTTACGCGCTGGGAACCGACAG
CGAGCTGCCCGAGCCGGTCATCCTGTGGGTGGCGAAGCAAGTCCAGGCCGAGCCGGCGAGCTGGGCAAAGTACGGCGAGCGCGACGTGACCCGTCGCGAG
CATGCCCAGGAACTGCGCACCTACCTGCAACTGGCCCCGTTCGGCCTGTCCGACTTCCGCGCCCTGGTGCGCGAGCTAACCGAGCTGGCCCAGCAGACCG
ACAAAGGCTTGCTGCTGGCCGGTCAGGCCCTGGAGAGCCTACGGCAGAAACGACGCATCCTGCCGGCGCTGAGCGTGATTGACCGGGCCTGCTCGGAAGC
CATTGCGCGAGCCAATCGGCGGGTCTACCGCGCCCTGGTCGAACCACTCACGGACTCGCATCGGGCCAAGCTGGACGAGCTGTTGAAGCTCAAGGCCGGC
AGCAGCATCACCTGGTTGACCTGGCTGCGCCAGGCACCGCTGAAACCCAACTCTCGGCACATGCTGGAACACATCGAGCGGCTGAAGACATTTCAGTTGG
TGGACTTGCCCGAAGGCCTGGGCCGGCACATCCACCAGAACCGCCTGCTCAAGCTGGCCCGCGAGGGTGGGCAGATGACGCCCAAAGACCTCGGTAAGTT
CGAGCCGCAGCGCCGCTACGCGACCCTGGCCGCCGTGGTGCTGGAGAGCACCGCGACCGTGATCGATGAGCTGGTCGATCTGCATGACCGCATCCTGGTC
AAGCTGTTCAGCGGCGCGAAGCACAAGCATCAGCAGCAGTTCCAGAAGCAGGGCAAGGCGATCAACGACAAGGTGCGCCTGTACTCCAGGATCGGCCAGG
CGCTGCTGGAAGCGAAGGAAAGCGGCAGCGACCCCTATGCCGCCATCGAGGCGGTGATTCCCTGGGACGAGTTCACCGAGAGCGTCAGCGAGGCCGAGCT
GCTGGCCCGGCCGGAAGGCTTCGACCACCTGCACCTGGTCGGCGAGAACTTCGCCACCCTGCGCCGTTACACGCCGGCCTTGCTGGAGGTGCTGGAACTG
CGCGCCGCGCCGGCCGCGCAAGGCGTGCTGGCAGCCGTGCAGACCCTGCGTGAGATGAACGCCGACAACCTGCGCAAGGTGCCGGCCGATGCACCCACGG
CCTTCATCAAGCCGCGCTGGAAGCCGCTGGTGATCACCCCGGAAGGCCTCGACCGGAAATTCTACGAAATCTGCGCCCTGTCCGAGCTGAAGAACGCCCT
GCGCTCCGGCGACATCTGGGTCAAGGGCTCGCGGCAGTTCCGCGACTTCGACGACTACCTGCTGCCGGCCGAGAAGTTCGCCGCACTCAAGCGCGAGCAG
GCCCTGCCCCTGGCGATCAACCCGAACAGCGACCAGTACCTGGAAGAGCGTTTGCAGCTGCTGGACGAGCAGTTGGCCACCGTCACCCGCCTGGCCAAGG
ACAACGAGCTGCCCGATGCCATCCTCACCGAGTCAGGGCTGAAAATCACCCCGCTGGATGCGGCGGTGCCGGATCGGGCGCAGGCGCTGATCGACCAAAC
CAGCCAGTTACTGCCGCGCATCAAGATCACCGAACTGCTGATGGACGTGGACGACTGGACGGGCTTCAGCCGCCACTTCACCCACTTGAAGGACGGGGCC
GAGGCCAAAGACAGGACGTTGCTGCTGTCCGCAATCCTCGGTGATGCGATCAACCTCGGGCTGACCAAGATGGCCGAGTCGAGCCCCGGCCTGACCTACG
CCAAGCTGTCCTGGCTGCAAGCCTGGCACATCCGCGACGAAACCTATTCGGCGGCCTTGGCCGAGCTGGTCAACCACCAGTATCGCCACGCCTTTGCCGC
CCACTGGGGCGACGGCACGACCTCATCCTCCGATGGCCAGCGCTTCCGCGCGGGTGGCCGGGGCGAGAGCACCGGGCACGTCAACCCGAAGTACGGTAGC
GAGCCGGGACGGCTGTTCTATACCCATATCTCCGACCAGTACGCGCCGTTCAGCACCCGCGTGGTGAATGTCGGCGTCCGCGATTCCACCTATGTGCTCG
ACGGCCTGCTGTACCACGAGTCCGACCTGCGGATCGAGGAGCACTACACCGACACGGCCGGCTTCACCGATCACGTCTTTGCCCTGATGCACCTGCTAGG
CTTCCGCTTCGCGCCGCGCATCCGCGACCTCGGCGAAACCAAGCTGTACGTGCCGCAGGGCGTGCAAGCCTACCCGACGTTGCGCCCGCTGATCGGCGGC
ACCCTGAACATCAAGCACGTGCGTGCCCACTGGGACGACATCCTGCGCCTGGCCAGCTCGATCAAGCAGGGCACCGTCACCGCCTCGCTGATGCTGCGCA
AGCTCGGCAGCTACCCGCGCCAGAACGGACTGGCCGTGGCCCTGCGCGAGCTGGGCCGGATCGAGCGCACGCTGTTCATCCTGGACTGGCTGCAAAGTGT
TGAACTGCGCCGCCGCGTGCATGCCGGCCTGAACAAAGGTGAGGCGCGCAACTCGCTGGCCAGGGCGGTGTTCTTCAACCGCCTTGGGGAAATCAGGGAT
CGGAGCTTCGAGCAGCAGCGCTACCGGGCCAGCGGCCTCAACCTGGTGACGGCGGCTATCGTGCTGTGGAACACGGTGTACCTGGAACGCGCCACCCAGG
GGTTGGTCGAGGCCGGCAAGCCGGTGGACGGCGAGCTGCTGCAATTCCTGTCGCCGCTGGGCTGGGAGCACATCAACCTAACCGGCGATTACGTCTGGCG
GCAGAGCCGCAGACTGGAAGACGGGAAGTTTCGGCCCTTACGGATGCCCGGAAAACCTTAGCGTACGATTTTTTCCGAATTCTGCGGGCTCCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 6

 

ORF 1
LengthBeginEndStrandFusion ORF
678 bp225 aa166843+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MKLNSDLSQRVVVEPSSLQWVDSPATGIQRQLLERDGDEVARATSIVRYAPGSAFENHKHDLGEEILVLDGEFSDESGTFGPGTYIKNPPGSSHAPSSAT
GCTLFVKLRHLDLADSQRTVVDTRNGPWFPGLVPGLSVMPLSEFDTQHTALVRWAPGTRFNSHRHYGGEEIYVLEGVFEDEFGSYPTGTWMRSPHLSAHR
PFSNTGCTILVKTGHLPVAESNEALA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
582 bp193 aa8431424+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MKHFSEISPTAERVVDAAEGLVQQHGYNGFSYDDVAQLVGIKKPSIHHHFPKKGELVAVVAQRYTHRFREELLSIEGQHAKAPDRLTAYAALFERTFAKD
RRLCVCGMLGAESDSLPDAVVSEVERFFKVNLDWLTLVVADGQRAALITSNSTPEALAEAFLCALEGSMMVGRGMRSSRGPAEVGNTFLSTVLT

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
774 bp257 aa14872260+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MSTEFNGKKLLVIGGTSGMGLQTARMVLEQGGSVVIVGHREDKAEEARKALSSLGTVTALTADLSRAEDVKRLLHTIDEHHKDINLLVNAAGVFFPKAFL
EHTESDYEQYLTLNKAFFFITQKVVANLVASERPGAIVNIGSMWGKQAIAATPSSAYSMAKAGLHSLTQHLAMELASKQIRVNAVSPAVVETPIYEGFIP
KAEVHGALQGFNSFHPIGRVGTPQDVAEVILFLLSDKAAWVTGAIWDVDGGVMAGRNT

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
579 bp192 aa22632841+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MGEREMNTNIKGTPGNGINVGALREFADQVAAKPAAGIATFGVVTTWEGGTRTRARTMPLVLGDTALARGFVIDADEPAELLGTDTAANPQELILAALNA
CMTATYAANAAAMNIELQSLTIRTKGSLDLRGFLGIDPGINPGYDQVEYEVEMESSADSAALKALHAQVQRTSPNYHNFARAIDLKAKLTILQ

 

Blast result :
ORF 5
LengthBeginEndStrandFusion ORF
558 bp185 aa31313688+No
ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQLEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGED
SPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEPKAQLAREFNISRETLYQYLRTDD

 

Blast result :
ORF 6
LengthBeginEndStrandFusion ORF
2964 bp987 aa36956658+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGDANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTR
REHAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALSVIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLK
AGSSITWLTWLRQAPLKPNSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAVVLESTATVIDELVDLHDRI
LVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIGQALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEVL
ELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKR
EQALPLAINPNSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKD
GAEAKDRTLLLSAILGDAINLGLTKMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRGESTGHVNPKY
GSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLI
GGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEI
RDRSFEQQRYRASGLNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMPGKP

 

Blast result :
Comments
TnAs1 ORF6 (transposase) is 96% aa similar to ISPa40 (TnPa40).
In the genome, this element is targetted by a cassette consisting of oppositely oriented terminal copies of the related elements ISApu1 and ISApu2 with an inverting 640 bp sequence.
ORFs 5 (resolvase) and 6 (transposase) are closely related to ORFs 4 and 5 of ISPa40.
ORFs 1-4 are passenger genes.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20